Disinfection method for explant of lycoris plants
The technology of a lycoris genus plant and a disinfection method is applied in the field of rapid plant propagation, can solve the problem of high pollution rate, and achieve the effects of reducing the pollution rate, high seed germination rate, and maintaining vitality
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[0033] Example 1
[0034] (1) Harvest the mature Huajinhua selfed fruits from September to October, soak them in water containing a little Tween-20 for 15 minutes, and rinse with running water for 2 hours; on a clean bench, clamp the fruits with tweezers After the stalk immersed the fruit in (v / v) 95% ethanol for 3 to 5 seconds, it was ignited on an alcohol lamp and burned until the pericarp was naturally extinguished. Then the whole seeds were peeled out with tweezers and a scalpel, and the seeds were used (v / v) Sterilize the surface with 75% alcohol for 30 seconds, then rinse with deionized water 3 times.
[0035] (2) Select the black, bright, mature seeds and place them in a clean cylindrical bottle filled with water, and the seeds can be submerged on the water surface, and then place the cylindrical bottle in a 37°C water bath for 6 hours.
[0036] (3) After the soaking is complete, put the seeds out and put them in a 50mL sterile centrifuge tube. The total volume of the seeds i...
Example Embodiment
[0042] Example 2
[0043] (1) Harvest mature Hudixiao selfed fruits from October to November, soak them in water containing a little Tween-20 for 20 minutes, and rinse with running water for 3 hours; on a clean bench, use tweezers to hold the stalk After immersing the fruit in (v / v) 95% ethanol for 3 to 5 seconds, ignite it on an alcohol lamp and burn until the peel is naturally extinguished. Then use tweezers and a scalpel to peel off the whole seeds. Use (v / v) 75 Sterilize the surface with% alcohol for 30 seconds, then rinse with deionized water 3 times.
[0044] (2) Select the black, bright, mature seeds and place them in a clean cylindrical bottle filled with water, and the seeds can be submerged on the water surface, and then place the cylindrical bottle in a 37°C water bath for 6 hours.
[0045] (3) After the soaking is complete, put the seeds out and put them in a 50mL sterile centrifuge tube. The total volume of the seeds in each tube is less than or equal to 1 / 3 of the cent...
Example Embodiment
[0051] Example 3
[0052] (1) Harvest the mature interspecific hybrid fruits of Lycoris radiata (female parent) and Huajinhua (male parent) from September to October, and soak them in water containing a little Tween-20 and detergent for 20 minutes. Rinse the fruit with running water for 3 hours; on an ultra-clean workbench, use tweezers to hold the stalk and immerse the fruit in (v / v) 95% ethanol for 3 to 5 seconds, then burn on an alcohol lamp until the peel naturally goes out. Use tweezers and The whole seeds were peeled off with a scalpel, and the seeds were surface-sterilized with (v / v) 75% alcohol for 30 seconds, and then rinsed with deionized water 3 times.
[0053] (2) Select the black, bright, mature seeds and place them in a clean cylindrical bottle filled with water, and the seeds can be submerged on the water surface, and then place the cylindrical bottle in a 37°C water bath for 6 hours.
[0054] (3) After the soaking is complete, put the seeds out and put them in a 50mL...
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