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Disinfection method for explant of lycoris plants

The technology of a lycoris genus plant and a disinfection method is applied in the field of rapid plant propagation, can solve the problem of high pollution rate, and achieve the effects of reducing the pollution rate, high seed germination rate, and maintaining vitality

Inactive Publication Date: 2015-11-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high contamination rate in the test, a better aseptic seeding sterilization method could not be screened out.

Method used

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  • Disinfection method for explant of lycoris plants
  • Disinfection method for explant of lycoris plants
  • Disinfection method for explant of lycoris plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Harvest mature Huanjinhua self-fertilized fruits from September to October, soak them in water containing a little Tween-20 for 15 minutes, and rinse them with running water for 2 hours; place them on an ultra-clean workbench and clamp the fruits with tweezers Submerge the fruit in (v / v) 95% ethanol for 3 to 5 seconds, light it on an alcohol lamp, burn until the pericarp goes out naturally, then peel off the complete seeds with tweezers and a scalpel, and use (v / v) Sterilize the surface with 75% alcohol for 30 seconds, then rinse with deionized water for 3 times.

[0035] (2) Select mature and plump seeds with black and bright color and put them in a clean cylindrical bottle filled with clean water. The water surface should not cover the seeds, and then place the cylindrical bottle in a water bath at 37°C for 6 hours.

[0036] (3) After soaking, clamp the seeds out and put them into 50mL sterilized centrifuge tubes. The total volume of seeds in each tube is less tha...

Embodiment 2

[0043] (1) Harvest the mature Hudixiao self-fertilized fruits from October to November, soak them in water containing a little Tween-20 for 20 minutes, rinse them with running water for 3 hours; place them on an ultra-clean workbench, and use tweezers to hold the fruit stalks After immersing the fruit in (v / v) 95% ethanol for 3-5 seconds, light it on an alcohol lamp, burn until the pericarp goes out naturally, then use tweezers and a scalpel to peel off the complete seeds, and spray the seeds with (v / v) 75 Sterilize the surface with % alcohol for 30 seconds, then rinse with deionized water 3 times.

[0044](2) Select mature and plump seeds with black and bright color and put them in a clean cylindrical bottle filled with clean water. The water surface should not cover the seeds, and then place the cylindrical bottle in a water bath at 37°C for 6 hours.

[0045] (3) After soaking, clamp the seeds out and put them into 50mL sterilized centrifuge tubes. The total volume of seeds ...

Embodiment 3

[0052] (1) Harvest the interspecies hybrid fruit of mature Lycoris (female parent) and Changjinhua (male parent) from September to October, soak in water containing a little Tween-20 and detergent for 20 minutes, Rinse with running water for 3 hours; on the ultra-clean workbench, clamp the fruit handle with tweezers, immerse the fruit in (v / v) 95% ethanol for 3-5 seconds, burn it on an alcohol lamp until the peel goes out naturally, use tweezers and The complete seeds were peeled off with a scalpel, the seeds were surface sterilized with (v / v) 75% alcohol for 30 seconds, and then rinsed 3 times with deionized water.

[0053] (2) Select mature and plump seeds with black and bright color and put them in a clean cylindrical bottle filled with clean water. The water surface should not cover the seeds, and then place the cylindrical bottle in a water bath at 37°C for 6 hours.

[0054] (3) After soaking, clamp the seeds out and put them into 50mL sterilized centrifuge tubes. The tot...

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Abstract

The invention discloses a disinfection method for an explant of lycoris plants. The disinfection method comprises the following steps of taking out seeds from lycoris plant fruits; disinfecting the surfaces of the seeds and then placing the seeds into warm water for soaking; imbibing the seeds, taking out the imbibed seeds and then placing the seeds into mercury bichloride for vortex oscillation centrifugal treatment; after the treatment, cleanly rinsing to obtain an aseptic explant. According to the disinfection method disclosed by the invention, after the seeds of the lycoris plants are soaked with warm water, the seeds fully absorb water and are imbibed, and then the imbibed seeds are placed into the mercury bichloride for short-time vortex oscillation, so that the mercury bichloride is fully infiltrated into the seeds, full disinfection can be realized, and the contamination rate of the lycoris plant seeds is reduced and is controlled to about 5.0 percent; in addition, the seed vigor can be kept, and a higher seed germination rate is obtained.

Description

technical field [0001] The invention belongs to the technical field of rapid plant propagation, and in particular relates to a disinfection method for plant explants of the genus Lycoris. Background technique [0002] The establishment of the aseptic sowing system of Lycoris plants is of great significance to its large-scale production and breeding, cross breeding and improved variety breeding, and the extraction of lycorine. [0003] The breeding research of Amaryllis plants is mostly focused on asexual reproduction, and seed reproduction is an important way to obtain natural variations such as flower color and flower shape of ornamental plants without affecting the growth of the mother bulb. Compared with substrate sowing, aseptic sowing can significantly increase the germination rate of seeds, promote the development and proliferation of primary bulbs, and obtain a large number of aseptic seedlings in a short period of time, which is also the key to solving the problem of...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 任梓铭夏宜平李岳李丹青毕波张栋
Owner ZHEJIANG UNIV
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