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Application of netrin-1 protein in preparing drug for treating tumor

1. Netrin-1, tumor treatment technology, applied in the field of netrin-1 protein in the preparation of drugs for tumor treatment, can solve the problems of lack of animal level experiments

Active Publication Date: 2015-11-11
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are only a few reports on the role of netrin-1 in the development of PDAC. It is only found that netrin-1 can promote the development of PDAC at the cellular level, but there is a lack of animal level experiments
Moreover, the role of netrin-1 in PDAC receptors, downstream signaling pathways, and effector genes regulated by netrin-1 are rarely studied.
There are few studies on the application of netrin-1 in the diagnosis, prevention and treatment of tumors, especially in the diagnosis, prevention and treatment of pancreatic ductal adenocarcinoma

Method used

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  • Application of netrin-1 protein in preparing drug for treating tumor
  • Application of netrin-1 protein in preparing drug for treating tumor
  • Application of netrin-1 protein in preparing drug for treating tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0058] 1. Experimental materials and methods

[0059] 1. Experimental cell lines and strains

[0060] 1.1 Tool cell lines

[0061] Human embryonic kidney HEK293T tool cell line (ATCC) was used for reverse transcription coating.

[0062] 1.2 Tumor cell lines

[0063] Two pancreatic cancer cell lines, MiaPacaII (ATCC) and CaPan1 (ATCC), were used to construct netrin-1 overexpression and other related molecular mutation pancreatic ductal adenocarcinoma cell lines.

[0064] 1.3 Experimental strains

[0065] DH5α competent Escherichia coli (TIANGEN) was used for virus construction and plasmid transformation.

[0066] 2 Experimental animals and feed

[0067] 2.1 Chicken embryo

[0068] Tumor formation experiments on CAM used chicken embryos from Whiteleghorn breeder eggs (Victoria).

[0069] 2.2 Immunodeficient mice

[0070] Tumor formation experiments in mice used male SCID-biege combined with severe immunodeficiency mice for tumor cell implantation. Tumor Growth Curve Det...

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Abstract

The invention discloses an application of netrin-1 protein in preparing a drug for treating tumor, wherein tumor is selected from pancreatic cancer, and preferably selected from pancreatic duct adenocarcinoma. The disclosure of the application has positive significances for preventing, diagnosing and treating pancreatic duct adenocarcinoma, and developing the drug for treating the pancreatic duct adenocarcinoma.

Description

technical field [0001] The present invention relates to the application of protein in tumor diagnosis and treatment, in particular to the application of netrin-1 protein in preparing medicine for tumor treatment. Background technique [0002] Pancreatic ductal adenocarcinoma (Pancreatic Ductal Adenocarcinoma, PDAC) is one of the deadliest tumors, in all pancreatic tumors, pancreatic ductal adenocarcinoma accounts for more than 95% of the proportion. Most cases of pancreatic ductal adenocarcinoma are advanced tumors or have already undergone distant metastasis when they are discovered. Only less than 20% of the patients can obtain the chance of surgical treatment, and the overall median survival time is less than 1 year. The pathogenesis of pancreatic cancer is poorly understood, which also limits the development of targeted treatment options and drugs. In recent years, the large-scale analysis of the expression changes and gene mutations at the molecular level of pancreatic...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K48/00A61P35/00G01N33/68G01N21/31
Inventor 吕湘刘德培安曦洲赵治国
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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