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Method for promoting activation and differentiation of cells

A cell and cell therapy technology, applied in biochemical equipment and methods, using electricity/wave energy to treat microorganisms, electricity/wave energy to treat enzymes, etc., can solve problems such as poor test and stimulation effects, and achieve good clinical application prospects, The effect of promoting cell growth and differentiation

Active Publication Date: 2015-11-11
吴剑波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current effect of measuring stimuli is not good and needs to be improved

Method used

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  • Method for promoting activation and differentiation of cells
  • Method for promoting activation and differentiation of cells
  • Method for promoting activation and differentiation of cells

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0028] Embodiment 1 The present invention promotes cell activation and differentiation method

[0029] The structure of the instrument for promoting cell activation and differentiation of the present invention—the cell therapy conversion instrument is as follows: figure 1 , 2 As shown, it includes a base, an outer cover 1 and an LED lamp tube 2 located inside the outer cover. There is a syringe inlet 3 on one side of the outer cover 1, a liquid crystal display 4 and an instrument start key 10 are arranged on the surface of the outer cover, and there is a housing needle inside the LED lamp tube 2. The channel 7 of the barrel, the opening of one end of the channel 7 is opposite to the inlet 3 of the syringe, the side of the channel 7 has an LED light source, the inside of the outer cover 1 has a control panel, the liquid crystal display 4, the LED light source and the instrument start key 10 are electrically connected to the control panel, The outer cover 1 is fixedly connected...

experiment example 1

[0039] Experimental example 1 adopts the method of the present invention to process platelet-rich plasma

[0040] 1. Instrument

[0041] The cell therapy conversion instrument described in Example 1 of the present invention.

[0042] 2. Processing method

[0043] Raw materials: Collect 10ml of venous peripheral blood from healthy people in a medical anticoagulant tube, immediately mix it upside down for 5-10 times, and centrifuge using the improved 2-time centrifugation method. The first centrifugation is at room temperature for 10 minutes to collect the supernatant; then the second time is centrifugation at room temperature for 1000g for 10 minutes. , Discard about 2 / 3 of the supernatant, and mix the remaining liquid to obtain 4-6ml of highly concentrated platelet-rich plasma (PRP).

[0044] The experimental groups are: the control group without LED light radiation (no treatment), the instrument LED light radiation group (in step (2), do not open the rotation control key at t...

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Abstract

The invention discloses a method for promoting activation and differentiation of cells. The method is characterized by processing by virtue of cell therapy transformation instrument through the following steps: (1) sampling cells, adding the cells into a syringe, and putting the syringe into a channel (7); and (2) turning on LED light sources and an instrument starting key (10) to illuminate for 10-20 minutes. According to the method, by virtue of combined radiation of red, green and yellow LED light sources with specific wave bands, the activation of the cells is efficiently induced, and the growth of the cells is promoted; the method is applicable to the regeneration and transformation of platelet-rich plasma (PRP), stem cells, epithelial cells and the like of human or animals and is good in clinical application prospect.

Description

technical field [0001] The present invention relates to a method for promoting cell activation and differentiation. Background technique [0002] LED (Light Emitting Diode) is a semiconductor solid light emitting device. When the semiconductor P-N junction is connected with a forward current, the carriers in the P region recombine, causing photon emission to directly convert electrical energy into light energy. At present, there are infrared, red, and Yellow, green, blue, purple and other light-emitting diodes. LED has the characteristics of cold light source, adjustable band, all solid state, high luminous efficiency, low energy consumption, low power, good monochromaticity, green environmental protection, long life, etc., and has been widely used in signal indication, decorative lighting, LCD backlight, communications and other fields. In recent years, with the great progress of LED light source technology, LED has become more and more widely used in the field of biomedi...

Claims

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Application Information

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IPC IPC(8): C12N13/00
Inventor 吴剑波罗茂
Owner 吴剑波