Method for determining optimal picking period of capsules of paphiopedilum concolor

A technology of the same color and picking period, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of poor capsule maturity, low seed germination rate, etc. simple effect

Inactive Publication Date: 2015-11-18
FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for determining the best harvesting period of the same-color Papuopodil capsules, which solves the problem that the seed germination rate is too low due to the poor maturity of the capsules during the conservation process of the same-color Paphiopedilum

Method used

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  • Method for determining optimal picking period of capsules of paphiopedilum concolor
  • Method for determining optimal picking period of capsules of paphiopedilum concolor
  • Method for determining optimal picking period of capsules of paphiopedilum concolor

Examples

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Embodiment 1

[0026] A method for determining the best picking period of Paphiopedilum capsules of the same color, comprising the following steps:

[0027] (1) Collection of fruit pods: pollinate the same color Paphiopedilum 4 days after flowering, and pick the fruit pods after 30-270 days of self-pollination as test materials;

[0028] (2) Fruit pod treatment: Wash the fruit pods with tap water, rinse with neutral detergent, wipe with alcohol, rinse with running water for 15 minutes, then transfer to an ultra-clean workbench, disinfect with 75% alcohol for 30 seconds, soak in 1% sodium hypochlorite solution for 12 minutes, Rinse 6 times with sterile water afterwards to obtain the treated fruit pods;

[0029] (3) Observation of inoculation and seed microstructure: cut open the treated fruit pods, take out the seeds, then inoculate a part of the seeds on the germination medium, and place the other part of the seeds under a microscope to observe the microstructure and embryo development; The...

Embodiment 2

[0033] A method for determining the best picking period of Paphiopedilum capsules of the same color, comprising the following steps:

[0034] (1) Collection of fruit pods: pollinate the same color Paphiopedilum 10 days after flowering, and pick the fruit pods as test materials after 30-270 days of self-pollination;

[0035] (2) Fruit pod treatment: Wash the fruit pods with tap water, rinse with neutral detergent, wipe with alcohol, rinse with running water for 15 minutes, then transfer to an ultra-clean workbench, disinfect with 75% alcohol for 30 seconds, soak in 1% sodium hypochlorite solution for 12 minutes, Rinse 6 times with sterile water afterwards to obtain the treated fruit pods;

[0036] (3) Observation of inoculation and seed microstructure: cut open the treated fruit pods, take out the seeds, then inoculate a part of the seeds on the germination medium, and place the other part of the seeds under a microscope to observe the microstructure and embryo development; Th...

Embodiment 3

[0040] A method for determining the best harvest period of Paphiopedilum capsules of the same color, comprising the following steps:

[0041](1) Collection of fruit pods: pollinate the same-color Paphiopedilum 6 days after flowering, and pick fruit pods after 30-270 days of self-pollination as test materials;

[0042] (2) Fruit pod treatment: Wash the fruit pods with tap water, rinse with neutral detergent, wipe with alcohol, rinse with running water for 15 minutes, then transfer to an ultra-clean workbench, disinfect with 75% alcohol for 30 seconds, soak in 1% sodium hypochlorite solution for 12 minutes, Rinse 6 times with sterile water afterwards to obtain the treated fruit pods;

[0043] (3) Observation of inoculation and seed microstructure: cut open the treated fruit pods, take out the seeds, then inoculate a part of the seeds on the germination medium, and place the other part of the seeds under a microscope to observe the microstructure and embryo development; The germ...

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Abstract

The invention provides a method for determining the optimal picking period of capsules of paphiopedilum concolor. The method comprises the following steps: collecting fruit pods, treating fruit pods, inoculating, observing the microstructure of seeds, carrying out statistics on sterile germination condition of the seeds, and determining the picking period. The method provided by the invention is easy and rapid to operate, the picking blindness of capsules of paphiopedilum concolor is avoided, the propagation cycle of paphiopedilum concolor is shortened, convenience is provided for conservation of paphiopedilum concolor, and reference is also provided for selection of the optimal maturity of other orchidaceae plants.

Description

technical field [0001] The invention belongs to the technical field of flower cultivation and tissue culture, and in particular relates to a method for determining the best picking period of the same-color Paphiopedilum capsule. Background technique [0002] Paphiopedilum concolor (Lindl.) Pfitz is a rare ornamental plant belonging to the genus Paphiopedilum of the Orchidaceae Cypripedilum subfamily. It is a protected object of Appendix I of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) trading. Paphiopedilum of the same color, terrestrial or epiphytic on stones, clustered plants, 4-7 leaves, oblong to narrowly elliptic, with grids of dark and light green or green and milky white on the leaves; scapes are nearly erect, 1- 3 flowers, mostly open one after another, the flowers are light yellow or nearly yellow, with fine purple spots, the flowering period is spring and summer, and it is an important hybrid parent of Paphiopedilum....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李秀玲卜朝阳王晓国黄昌艳周锦业邓杰玲周主贵宋倩黄凤玲罗海斌
Owner FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI
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