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A rhizobia and seed technology, applied in applications, bacteria, agriculture, etc., can solve the problems of high salinity and lack of fertilizer sources in saline-alkali land, and achieve the characteristics of improving saline-alkali land, significant growth promotion effect, and efficient nodulation and nitrogen fixation. Effect
Active Publication Date: 2019-03-01
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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[0005] Tianjing is often used to improve saline-alkali land, and saline-alkali land not only has high saline-alkali content, but also faces a lack of fertilizer sources
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Embodiment 1
[0023] Example 1: Obtaining Rhizobium
[0024] From the yellow river delta saline-alkaliized soil planting field, select the sesame seeds that grow vigorously above and below the ground, collect the nodules, disinfect the surface of the nodules, mash the nodules, and spread the extract on the YMA plate, and culture them upside down at 28℃3 -5 days, when round colonies with neat edges, milky white translucent, and more extracellular polysaccharides grow, pick a single colony and streak repeatedly until it is purified (see figure 1 ).
[0025] The formula of YMA solid medium (yeast extract mannitol agar medium) is: mannitol 10g / L, yeast extract 3g / L, K 2 HPO 4 0.25g / L, KH 2 PO 4 0.25g / L, MgSO 4 0.2g / L, CaCO 3 3g / L, NaCl 0.1g / L, deionized water to a constant volume of 1000mL, and dispense them into Erlenmeyer flasks. Add agar powder with a final concentration of 20g / L to each bottle.
[0026] 1) Identification of rhizobia
[0027] Gram staining and morphological identification: The r...
Embodiment 2
[0039] Example 2 Preparation and application of bacterial agent of strain YIC4027
[0040] 1) Strain activation
[0041] Use the sterilized inoculation loop to pick YIC4027 colonies, and streak them on YMA solid medium, invert the culture at 28°C for 2-3 days until the colonies grow well.
[0042] 2) Expand cultivation
[0043] Use the sterilized inoculating loop to pick one or two loops of bacteria, and inoculate them into 200 mL of sterilized liquid YMA liquid medium, and culture with shaking at 28°C and 160 rpm for 24 to 48 hours, and cultivate until OD600≥1.5. It is the inoculant to be used. Put the inocula in a refrigerator at 4℃ for 1 week.
[0044] 3) Application of rhizobia
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Abstract
The invention relates to a technology for improving a saline and alkaline land and particularly relates to rhizobium and an application thereof. The rhizobium (Ensifersp.) YIC4027 has been collected in China General Microbiological Culture Collection Center (short for CGMCC and the address is No. 3, No. 1 yard, Beichen West Road, Chaoyang District, Beijing), and the collection number is CGMCC No. CGMCC 10994. The rhizobium has the advantages that the strain growth speed is fast, saline and alkaline resistance is achieved, effective symbiotic nitrogen fixation with sesbania can be achieved, the growth of the sesbania can be obviously promoted under the environment of greenhouses and large fields, the yield of above-ground parts and under-ground parts of plants can be obviously increased, and the yield and the quality of the sesbania are improved; the benefit for planting the sesbania to improve the saline and alkaline lands and other barren lands is achieved, the use of fertilizer is reduced, the environmental pollution caused by the fertilizer is reduced and finally the purposes of improving the saline and alkaline soil and barren soil, promoting ecological restoration and protecting the ecological environment are achieved.
Description
Technical field [0001] The invention relates to a technique for improving saline-alkali soils, specifically a rhizobia and its application. Background technique [0002] Rhizobium is a kind of gram-negative bacteria that can form nodules or stem nodules with legumes to fix nitrogen in symbiosis. When rhizobia coexist with matching legumes, an effective symbiotic tissue is formed-nodules or stem nodules. In the nodules or stem nodules, the rhizobia convert the nitrogen in the air into ammonia and provide it to the host plant as a nitrogen source. Leguminous plants provide carbon sources and nutrients for rhizobia, and the two form a good symbiotic relationship with each other. [0003] Because rhizobia and legumes need to experience a series of interactive molecular dialogues during the interaction process, and this symbiosis process will be affected by environmental factors, the indigenous high-efficiency nitrogen-fixing rhizobia will better adapt to the local environment and inte...
Claims
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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/04C05G3/80
Inventor 李岩解志红李项岳任承钢刘卫吴海龙姜南
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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