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A kind of determination method of natamycin content in wine

A technology of natamycin and determination method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of natamycin detection patents, etc., and achieve the effect of reliable method, convenient method and reliable implementation.

Active Publication Date: 2017-05-17
CHINA AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant patent for the detection of natamycin in wine

Method used

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  • A kind of determination method of natamycin content in wine
  • A kind of determination method of natamycin content in wine
  • A kind of determination method of natamycin content in wine

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Embodiment 1

[0089] Precision and recovery detection of natamycin content in wine:

[0090] Two kinds of wine samples were prepared: real wine sample and spiked recovered wine sample, the spiked recovered wine sample was used to verify the reliability of the determination method of wine natamycin content;

[0091] Real wine samples: original red and white wine, no treatment;

[0092] Spiked and recovered wine samples: Add 10, 20, and 50 μg / L natamycin A to red and white wines respectively to prepare spiked and recovered wine samples for later use.

[0093] (1) Activation: respectively activate the WAX ​​solid phase extraction cartridge with 3ml methanol and 3ml water respectively;

[0094] (2) adjust the pH value of the wine sample: adjust the pH of the real wine sample and the spiked recovery wine sample to be 8 with ammonia water with a concentration of 25%;

[0095] (3) Sample loading: Inject the real wine sample after pH adjustment and the spiked recovered wine sample into the WAX ​​...

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Abstract

The invention belongs to a field of foodstuff detection and foodstuff safety, and relates to a method for determining content of natamycin in wine, especially relates to a method which employs a solid phase extraction column for purification and a HPLC-MS / MS method for detection. The method comprises the following steps: (1) activation, (2) adjustment of wine sample pH value, (3) sampling, (4) leaching, (5) elution, (6) drafting of a natamycin standard curve, (7) calculation of the content of natamycin in the wine sample. According to the invention, the method for detecting the content of natamycin in wine is rapid and effective, the detection time is 5 minutes, the detection limit 1mug / l, the quantification limit is 3.34mug / l, and the error is 2-4%. Besides, because the HPLC-MS-MS technology is used, the false positive is effectively eliminated.

Description

technical field [0001] The invention belongs to the field of food detection and food safety, and relates to a method for determining the content of natamycin in wine, in particular to a method for purification through solid phase extraction column and detection through high performance liquid chromatography-mass spectrometry method. Background technique [0002] Natamycin was first discovered in 1955. It is also called Pimaricin, streptomycin or pimaricin. It is an important polyene macrolide antibiotic. There are many production ways, and it can be produced by fermentation of various Streptomyces such as Streptomyces Natalensisi, Streptomyces chmanovgensis and Streptomyces gilvosporeus. It is a natural Polyene macrolide biological antibacterial agent and food additive. In the early stage of discovery, natamycin was mainly used in medicine, and it was gradually included in the ranks of food preservatives after 1960. In 1976, 1982 and 1996 respectively, it was approved by ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 战吉宬韩深李雪银王佩玥孙翔宇黄卫东
Owner CHINA AGRI UNIV
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