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Method for effectively inducing stem cells to differentiate into pancreas islet beta cells

A cell differentiation and stem cell technology, applied in the field of biomedicine to achieve the effect of improving vitality

Active Publication Date: 2015-11-25
夏亮
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cell aggregation undoubtedly provides a more natural environment for differentiation. There is currently no report on the use of MET agonists to effectively stimulate the differentiation of stem cell clusters into pancreatic β-like cells.

Method used

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  • Method for effectively inducing stem cells to differentiate into pancreas islet beta cells
  • Method for effectively inducing stem cells to differentiate into pancreas islet beta cells

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Embodiment 1

[0028] A method for efficiently inducing stem cells to differentiate into pancreatic beta-like cells, the method comprising the following steps:

[0029] 1. Umbilical cord blood mesenchymal stem cells were cultured on 2% gelatin-coated culture plates, and cultured in DMEM (Life Technologies, Gaithersburg, MD, USA) containing 20% ​​fetal bovine serum (Ultrapureagarose, Invitrogen, Carlsbad, CA) for 7 days. , the induction begins when the average diameter of cell spheres reaches 100 microns.

[0030] 2. The cells were washed twice with PBS, and cultured for 24 hours using medium I. The components of medium I were: DMEM / F12 (Life Technologies), 1 mM sodium butyrate (Sigma, St.Louis, MO, USA), 50 ng / mL ActivinA (Sigma).

[0031] 3. After 24 hours, wash the cells with PBS and replace with medium II, and continue to culture for 48 hours. The components of medium II are: DMEM / F12, 0.1-0.2% fetal bovine serum, 50ng / mL ActivinA.

[0032] 4. After 48 hours, wash the cells with PBS, an...

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Abstract

The invention discloses a method for effectively inducing stem cells to differentiate into pancreas islet beta cells. The method comprises the following steps: under the condition of inducing cell agglomeration, firstly treating the cell cluster through adopting an MET agonist, so as to achieve the method for effectively inducing stem cells to differentiate into pancreas islet beta cells. According to the method, the efficiency and vigour of stem cells differentiation into insulin secretory cells are improved, and hyperglycemia caused by the situations that beta cells are damaged and the absolute amount of insulin secretion decreases is treated.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for inducing stem cells to aggregate and treating the cell cluster with a MET agonist to efficiently induce stem cells to differentiate into pancreatic islet β-like cells. Background technique [0002] Type I diabetes is caused by insufficient insulin secretion due to damage to insulin-secreting β cells due to poisoning or autoimmune attacks. People with diabetes suffer from obesity, high blood sugar, limb movement disorders, kidney damage and eye diseases. For many years, tissue- and cell-based regenerative alternative treatments have been sought for the eradication of diabetes. Among them, stem cell therapy has attracted much attention in recent years. It is believed that inducing stem cells to become insulin-secreting cells and transplanting them is a reliable way for stem cells to treat diabetes. There are many ways to induce stem cells to differentiate into islet β-lik...

Claims

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Application Information

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IPC IPC(8): C12N5/071
Inventor 孙博肖忠党
Owner 夏亮
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