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Immunologic adjuvant of veterinary vaccine as well as preparation method and application thereof

An immune adjuvant and vaccine technology, applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, antibody medical components, etc., to achieve the effect of solving vaccine side effects, easy injection, and broad application prospects

Active Publication Date: 2015-12-02
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies on polysaccharides as immune adjuvants are limited to microbial sources of lipopolysaccharide and glucose

Method used

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  • Immunologic adjuvant of veterinary vaccine as well as preparation method and application thereof
  • Immunologic adjuvant of veterinary vaccine as well as preparation method and application thereof
  • Immunologic adjuvant of veterinary vaccine as well as preparation method and application thereof

Examples

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preparation example Construction

[0025] Aiming at the problem of developing a new adjuvant, the invention provides an immune adjuvant for a veterinary vaccine, a preparation method and an application thereof.

[0026] Pine pollen polysaccharides are composed of monosaccharides such as mannose, glucuronic acid, rhamnose, glucose, galactose, arabinose and fucose. Modern research has proved that pine pollen polysaccharide is one of the nutrients that maintain the special effects of pine pollen, and has very high biological activity. The extraction of polysaccharides from pine pollen is significantly increased after the wall is broken. In the present invention, fresh pine pollen in Mount Tai area is collected in spring, sieved and broken, and the crude polysaccharide is extracted by water extraction and alcohol precipitation. During the extraction process, protein is removed by pepsin and Sevage reagent, which ensures a higher purity of the crude polysaccharide.

[0027] A variety of plant polysaccharides can not...

Embodiment 1

[0030] Example 1 Adjuvant 1

[0031] An immune adjuvant for veterinary vaccines. The immune adjuvant is composed of the following raw materials in parts by mass: 5 parts of Taishan pine pollen polysaccharide, 10 parts of propolis, 5 parts of aloe polysaccharide, 8 parts of raspberry polysaccharide, 6 parts of Laojunxu polysaccharide, 9 parts of Dawanhua polysaccharide, 10 parts of hydrophilic surfactant, 0.1 part of polymer micellar substance and 40 parts of water.

[0032] Wherein, the hydrophilic surfactant is a mixture of Span 80 and Pluronic 121; the mass-number ratio of Span 80 and Pluronic 121 is 1 part for Span 80 and 3 parts for Pluronic 121.

[0033] Wherein, the polymer micelle substance is a mixture of chitosan and carbomer 971P; the mass and number ratio of chitosan and carbomer 971P is 1 part for chitosan and 3 parts for carbomer 971P. share.

[0034] The preparation method of the immune adjuvant of the veterinary vaccine comprises the following steps: weighing ...

Embodiment 2

[0041] Embodiment 2 Adjuvant 2

[0042] An immune adjuvant for veterinary vaccines, wherein the immune adjuvant is composed of the following raw materials in parts by mass: 30 parts of Taishan pine pollen polysaccharide, 5 parts of propolis, 10 parts of aloe polysaccharide, 14 parts of raspberry polysaccharide, and 3 parts of Laojunxu polysaccharide , 4 parts of Dawanhua polysaccharide, 0.1 part of hydrophilic surfactant, 10 parts of polymer micelles and 90 parts of water.

[0043] Wherein, the hydrophilic surfactant is a mixture of Span 80 and Pluronic 121; the mass-number ratio of Span 80 and Pluronic 121 is 2.5 parts for Span 80 and 1 part for Pluronic 121.

[0044] Wherein, the polymer micelle substance is a mixture of chitosan and carbomer 971P; the mass ratio of chitosan and carbomer 971P is 3 parts for chitosan and 2 parts for carbomer 971P.

[0045] The preparation method of the immune adjuvant of the veterinary vaccine comprises the following steps: weighing each com...

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Abstract

The invention discloses an immunologic adjuvant of veterinary vaccine as well as a preparation method and application thereof. The immunologic adjuvant comprises the following raw materials: Taishan pine pollen polysaccharide, propolis, aloe polysaccharide, raspberry polysaccharide, cynanchum mooreanum polysaccharide, calystegia hederacea polysaccharide, a hydrophilic surfactant, a polymeric micelle substance and water. The immunologic adjuvant has the benefits as follows: the immunologic adjuvant is simple in preparation technology, low in cost, simple in ingredients, stable in dosage form, easy to store, easy for injection, convenient to use, excellent in action effect and free of toxic and side effects, and has huge development utilization potential in animal husbandry, thereby having a wide application prospect.

Description

technical field [0001] The invention relates to a vaccine adjuvant, in particular to an immune adjuvant for a veterinary vaccine, a preparation method and application thereof. Background technique [0002] As a non-specific immune enhancer, adjuvant can enhance the body's immune response ability or change the type of immune response. A vaccine with a certain immune effect often needs a matching adjuvant to better exert the vaccine's anti-infection properties. In recent years, with the advancement of modern biotechnology, the research of various new vaccines (such as: subunit vaccines, genetic engineering vaccines) has gradually become a hot topic, but the research on immune adjuvants is obviously lagging behind, which is far from meeting the needs of the development of new vaccines. need. Although traditional immune adjuvants such as white oil adjuvants and aluminum hydroxide gel adjuvants can improve the efficacy of vaccines to a certain extent, these traditional adjuva...

Claims

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Application Information

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IPC IPC(8): A61K39/39
Inventor 朱瑞良魏凯胡莉萍钟世勋彭军
Owner SHANDONG AGRICULTURAL UNIVERSITY
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