A kind of germplasm preservation method of dahlia tissue culture propagation

Abstract

The invention discloses a germplasm preservation method for tissue culture propagation of dahlia, comprising the following technical links: 1) material selection and low-temperature pretreatment of explants; 2) inoculation; 3) induction and transfer of adventitious buds; 4) slow growth Preservation of germplasm; 5) Rejuvenation; 6) Rooting and transplanting. The germplasm preservation method for tissue culture propagation of dahlia provided by the invention has a period of up to 4 years for the preservation of dahlia germplasm, and at the same time solves the problems of large area of ​​germplasm preservation and high cost, and is easy to operate, stable and reliable, and repeatable It is very suitable for the preservation of large-scale dahlia germplasm, and is an excellent method for the preservation of dahlia germplasm resources.

Description

technical field [0001] The invention relates to a germplasm preservation method of dahlia tissue culture propagation, which belongs to the field of agricultural science and technology. Background technique [0002] Dahlia ( Dahlia pinnata Cav) is a perennial bulbous flower of the genus Dahlia in the Asteraceae family. It belongs to about 27 species. It is also known as dahlia chrysanthemum, geranium peony, passion flower, peony, dahlia, sweet potato flower, sweet potato flower, etc. It is native to Mexico, Colombia and Guatemala. and other countries, and later spread to Europe, Japan and my country. Dahlia has thick stems, large leaves, similar flower shape to peony, large and colorful flowers, beautiful flowers, various flower shapes, long flowering period, and various varieties. It is known as the darling of flowers. Dahlia is a natural interspecies hybrid, and the history of artificial breeding is not long. It is widely cultivated in countries all over the world. At pre...

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Problems solved by technology

[0004] At present, most dahlia germplasm resources are preserved by field cultivation, but field cultivation has the following limitations: it is difficult to control light, temperature, water, and pests and diseases, resulting in the loss of germplasm resources; it occupies a large area and consumes manpower, material resources, and Huge financial resources; prone to artificial mixing and species degradation in the long-term asexual reproduction process

At present, there is no mature dahlia germplasm preservation system applied to the industrial development of dahlias. Therefore, in view of the existing shortage of manpower, material resources, space consumption and short preservation time of dahlias, a suitable, long-term preservation, and The low-cost dahlia germplasm resource preservation method is of great significance to the industrial development of dahlia

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