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A scaffold material with gradient activity for osteochondral interface repair and its preparation method and application

A scaffold material and bone cartilage technology, applied in the field of scaffold materials with gradient activity and its preparation, can solve the problems of unquantifiable production, insufficient mechanical properties, cumbersome operation, etc., achieve low cost, promote bone cell calcium absorption, and preparation process simple effect

Inactive Publication Date: 2018-07-31
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem solved by the present invention is to overcome the shortcomings of existing tissue engineering scaffold materials such as single structure, insufficient mechanical properties, cumbersome operation, and inability to quantify production, etc., to provide a scaffold material with gradient activity for osteocartilage interface repair and Its preparation method and application

Method used

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  • A scaffold material with gradient activity for osteochondral interface repair and its preparation method and application
  • A scaffold material with gradient activity for osteochondral interface repair and its preparation method and application
  • A scaffold material with gradient activity for osteochondral interface repair and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 casein / calcium carbonate microsphere

[0041] Prepare 50 mM calcium salt (calcium chloride) solution and 50 mM carbonate (sodium carbonate and 8 mg / ml casein), mix the two solutions in equal volumes, stir at 600 rpm for 20 min, wash repeatedly three times and Freeze dried. The material was fixed on the sample stage, sprayed with gold, placed in the vacuum chamber of the thermal field emission scanning electron microscope, and observed at a voltage of 15 kV to obtain the SEM observation picture. products such as figure 1 As shown, the microspheres are uniformly dispersed, and the size is about 1 μm, which is a uniform spherical shape.

Embodiment 2

[0042] Example 2 PCL / CaCO 3 Electrospun membrane preparation

[0043] Weigh a certain mass of casein-calcium carbonate composite microspheres, disperse them in DMF at a concentration of 20% (w / v), and stir thoroughly to obtain suspension A; meanwhile, weigh twice the mass of calcium carbonate composite microspheres in PCL to dissolve In DCM, get solution B; add A to B, wherein the volume ratio of DMF and DCM is 1:3, and the final concentration of the system is 10% PCL, 5% CaCO 3 , after stirring for 12~72 h, absorb a certain amount for spinning, the driving speed is 1.0ml / h, the applied voltage value is 14kV, and the receiving distance is 11cm. After the spinning material was dried naturally, the spinning membrane was soaked in 1.5 M sodium hydroxide solution at 0-4 °C for 40 min-1 h, washed with deionized water three times, dried naturally and stored for later use.

[0044] The above samples were sterilized by immersing them in 75% alcohol for 1-3 h, and then air-dried natu...

Embodiment 3

[0045] Example 3 PLGA / CaCO 3 Composite cast film preparation

[0046] Weigh a certain mass of casein calcium carbonate composite microspheres, disperse them in DMF at a concentration of 20% (w / v), and stir them thoroughly to obtain suspension A; in DCM to obtain solution B; add A to B, wherein the volume ratio of DMF and DCM is 1:3, the final concentration of the system is 10% PLGA, 5% CaCO3, after stirring for 12~72 h, the PLGA / CaCO 3 12 mL of the mixture was placed in a clean glass Petri dish, shaken slightly to make it uniform, dried in a vacuum oven at room temperature for 2–48 h, and soaked in 1.5 M sodium hydroxide solution at 0–4°C for 10 After min~20 min, wash with deionized water three times, dry naturally and save for later use.

[0047] The above samples were sterilized by immersing them in 75% alcohol for 1-3 h, and then air-dried naturally for 12-24 h, then added sterile SBF solution into a 15 mL centrifuge tube, changed the liquid every other day, and soaked at...

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Abstract

The invention discloses a stent material with gradient activity for osteochondral interface restoration, a preparation method of the stent material and application of the stent material. The stent material is prepared by the following preparation method that: S1, a calcium salt solution and a casein-containing carbonate solution are mixed; stirring, cleaning and drying are carried out; then, casein-containing composite calcium carbonate microspheres are obtained; S2, the casein-containing composite calcium carbonate microspheres in the S1 are dispersed into N, N-dimethylformamide to obtain suspension A; biomedical medical biodegradable polymers are dissolved into dichloromethane to obtain a solution B; the A is added into the B; stirring is carried out; preparation and film forming are carried out; after the drying, film forming materials are soaked in an alkaline solution for use; S3, materials obtained in the S2 are added into sterile a SBF (simulated body fluid) solution for mineralization, cleaning and drying; the stent material is obtained. The stent material prepared by the invention has the hydroxyapatite gradient form formed after the mineralization; the biocompatibility and the mechanical property are good; the mass production can be easily realized.

Description

technical field [0001] The invention relates to the technical field of biomaterials, more specifically, to a scaffold material with gradient activity for osteocartilage interface repair and its preparation method and application. Background technique [0002] Articular cartilage is composed of dense connective tissue containing collagen fibers to form a basic frame, which is semi-circular in shape, with the bottom end tightly attached to the bone and the upper end facing the articular surface. This structure makes the cartilage and bone tightly combined so that it is difficult to fall off. When under pressure, a small amount of deformation is allowed to play the role of buffering pressure. Between the collagen fibers, there are gradually changing chondrocytes. From the superficial layer to the deep layer, the chondrocytes gradually transition from flat to oval or round to maintain the normal metabolism of cartilage. Cartilage can be divided into four layers. The shape and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/04A61L27/18A61L27/54
Inventor 李燕曹智楠王丹丹马双双蒋庆龚逸鸿叶舒莹
Owner SUN YAT SEN UNIV
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