Supercharge Your Innovation With Domain-Expert AI Agents!

Separation method of saponin compound

A separation method and compound technology, which are applied in the directions of steroids, chemical instruments and methods, and steroid preparation, can solve the problems of high cost and low yield, and achieve the effects of low cost, material economy and process stability.

Active Publication Date: 2015-12-09
GUANGXI MEDICAL UNIVERSITY
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Saponins (CAS: 290809-72-2) were isolated for the first time by Japanese AkihitoYokosuka (AkihitoYokosuka, YoshihiroMimaki, YutakaSashida.SpirostanolsaponinsfromtherhizomesofTacchantryerianandtheircytotoxicactivity[J].Phytochemistry61(2002):73-78), the separation method is relatively low in cost and yield It is not targeted to extract and separate the saponin monomer, and it is only suitable for laboratory research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for separating saponins, comprising the following steps:

[0018] A, konjac plant split fruit potato (Latin scientific name: Taccaplantaginea (Hance) Drenth) is dried and crushed, then add 9 times the amount by weight, and the volume concentration is 100% ethanol, heated and refluxed for 3 times, each extraction 2h, filter, combine the extracts, and recover the solvent by rotary evaporation to obtain the crude extract of yam;

[0019] B. According to the ratio of crude extract: purified water weight ratio of 1:10, add purified water to the crude extract to obtain a suspension mixture, then sequentially extract with petroleum ether, ethyl acetate, n-butanol, and recover the solvent by rotary evaporation , to obtain four parts extracts of petroleum ether part, ethyl acetate part, n-butanol part and water part;

[0020] C, the n-butanol part passes through the D101 macroporous adsorption resin column, the sample is dissolved and loaded with ethanol with a volume ...

Embodiment 2

[0024] A method for separating saponins, comprising the following steps:

[0025] A, konjac plant split fruit potato (Latin scientific name: Taccaplantaginea (Hance) Drenth) is dried and then pulverized, then add 10 times the amount by weight, and the volume concentration is 80% ethanol, heated and refluxed for 3 times, each extraction 2.2h, filter, combine the extracts, and recover the solvent by rotary evaporation to obtain the crude arrowroot potato extract;

[0026] B. According to the ratio of crude extract: purified water weight ratio of 1:10, add purified water to the crude extract to obtain a suspension mixture, then extract with petroleum ether, ethyl acetate, and n-butanol in sequence, and recover the solvent by rotary evaporation , to obtain four parts extracts of petroleum ether part, ethyl acetate part, n-butanol part and water part;

[0027] C, the n-butanol part passes through the D101 macroporous adsorption resin column, the sample is dissolved and loaded with...

Embodiment 3

[0031] A method for separating saponins, comprising the following steps:

[0032] A, konjac plant arrow root potato (Latin scientific name: TaccachantryeriAndre.) is dried and then pulverized, then add 11 times the amount by weight, and the ethanol with a volume concentration of 70%% is heated and refluxed to extract 3 times, each time extracting 2.3 h, filtering, combining the extracts, and recovering the solvent by rotary evaporation to obtain the crude extract of yam;

[0033] B. According to the ratio of crude extract: purified water weight ratio of 1:10, add purified water to the crude extract to obtain a suspension mixture, then extract with petroleum ether, ethyl acetate, and n-butanol in sequence, and recover the solvent by rotary evaporation , to obtain four parts extracts of petroleum ether part, ethyl acetate part, n-butanol part and water part;

[0034] C. The n-butanol part passes through the D101 macroporous adsorption resin column, the sample is dissolved and l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of natural medicines and particularly relates to a separation method of saponin compounds. In the method, a taccaceae plant is employed as a raw material to prepare a saponin monomer being higher than 90% in purity through the steps of crushing, ethanol extraction, extractions with petroleum ether, ethyl acetate and n-butyl alcohol, D101 macro resin elution and elution in silica gel column and gel column. The separation method is simple in operation, is high in separation efficiency, is high in purity, is stable in process, is low in cost, employs economical and recyclable raw materials and provides fundament for development and utilization to the saponin compound.

Description

technical field [0001] The invention belongs to the technical field of natural medicine extraction, and in particular relates to a method for separating saponins. Background technique [0002] Konjac Yam (Latin scientific name: TACCACEAE) plants are perennial herbs with cylindrical or spherical rhizomes or tubers. The leaves are all basal, stalked, erect, and sheathed at the base; the leaves are entire or divided in various ways. The flowers are bisexual, radially symmetrical, arranged in umbels, and grow on long scapes; the type genus of the family: Konjac Yam TaccaJ.R. tropics. There are 2 genera and 6 species in China, distributed from southeast to southwest. The components of the Konjac Yasaceae plant are complex, and most of the components have been successfully separated at present. It is found that the Konjac Yasminaceae plant is rich in saponins and arrow ketones, and these substances have good biological activities. Anti-tumor effect, and can regulate the microt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07J75/00C07J71/00
Inventor 梁钢孙悦文欧明春刘布鸣邱汉琛陈燕燕
Owner GUANGXI MEDICAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com