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A kind of preparation method of dextran microsphere gel

A technology of dextran and microspheres, applied in the field of preparation of hydrogel microspheres, can solve the problems of excessive hydrolysis of PVAc, unfavorable industrial production, difficult process control, etc., and achieve stable properties, narrow particle size distribution range, large range of control

Active Publication Date: 2018-01-16
南雄阳普医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is necessary to find a suitable alkaline point, which is beneficial to the cross-linking of dextran and cannot cause excessive hydrolysis of PVAc. There are too many influencing factors to be considered in this process, and the process is difficult to control, which is not conducive to industrial production.

Method used

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  • A kind of preparation method of dextran microsphere gel
  • A kind of preparation method of dextran microsphere gel
  • A kind of preparation method of dextran microsphere gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] This embodiment includes a specific pore size cross-linked dextran microsphere gel synthesis technology, including the following steps:

[0058] 1) Prepare 2.5M sodium hydroxide solution: weigh 10 g of sodium hydroxide, add 100 ml of distilled water, and stir until colorless and transparent. Weigh 6.0±0.5g into a clean container;

[0059] 2) Prepare the dispersed phase: Weigh 3.0±0.3g of dextran, add it to the above 2.5M sodium hydroxide solution, and stir under the homogeneous emulsification equipment. The stirring parameter is 600±100rpm for 3-5min. The sugar is completely dissolved into a colorless and transparent solution, which is used as the dispersed phase for later use.

[0060] 3) Weigh 60±5g of castor oil, put it in a clean container, and set aside.

[0061] 4) Preparation of the continuous phase: Weigh 2.5±0.3g of Span, add it to the above-mentioned castor oil, and stir under the homogeneous emulsification equipment. The stirring parameter is 600±100rpm, 3-...

Embodiment 2

[0071] The dextran microsphere gel of this example is basically the same as that of Example 1, the difference being that the concentration of the dispersed phase is changed: in this example, the mass of dextran added in step 2) is 6.0±0.6g.

[0072] 100 times biological microscope observes the morphology of the dextran microspheres prepared by the method of the present embodiment, such as Figure 4 , three microspheres were randomly selected for measurement, and their radii were respectively 39.62 μm, 23.08 μm, and 48.40 μm; , samples were taken to measure the particle size range of the gel microspheres, and the particle size distribution range was as follows Figure 5 ,, is 40-120μm, can be used in serological detection in the field of clinical diagnosis, such as micro-column gel method to detect infectious disease markers, cancer markers, etc., and for proteins, polysaccharides, nucleic acids of different molecular weights Separation and purification of substances such as en...

Embodiment 3

[0074] The dextran microsphere gel of the present embodiment is basically the same as that of Example 1, the difference is that the dispersion and emulsification speed is changed: in the present embodiment, step 6) adjusts the parameters of the homogeneous emulsification equipment to 600 ± 100rpm, 25- 30min.

[0075] 100 times biological microscope observes the morphology of the dextran microspheres prepared by the method of the present embodiment, such as Figure 6, randomly selected three microspheres for measurement, and their radii were respectively 23.60 μm, 33.94 μm, and 49.81 μm. Samples were taken to measure and count the particle size range of the gel microspheres. The particle size distribution range is as follows Figure 7 , which is 40-120μm, can be used in serological detection in the field of clinical diagnosis, such as microcolumn gel method to detect specific size infectious disease markers, cancer markers, etc., and for proteins, polysaccharides, nucleic acids...

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Abstract

The invention discloses a preparation method of dextran microsphere gel. The steps are as follows: separately prepare a dispersed-phase dextran solution and a continuous-phase solution; drop the dispersed-phase solution into the continuous-phase solution at a uniform speed; High-quality emulsification equipment is used to fully emulsify the mixture; add a cross-linking agent and mechanically stir to fully cross-link the mixture to obtain dextran microsphere gel. The dextran microsphere gel of the present invention selects common domestic raw materials, its price is low, the supply cycle is short, the process is simple and large-scale production is possible, there is no hidden danger of safe production and environmental pollution, and it is widely used in different detection technologies, especially greatly promoting the production of microspheres. The popularization and application of column gel detection technology in China has improved the medical level.

Description

technical field [0001] The invention relates to a method for preparing hydrogel microspheres, in particular to a method for preparing cross-linked dextran microsphere gels with specific apertures. Background technique [0002] Micro-column gel technology was invented by the French Dr. Yves Lappierre in 1986 and can be used for blood group serological detection. It was certified by the US FDA in 1994 and has been used clinically as a routine technology in many developed countries. It belongs to size exclusion chromatography (size exclusion chromatography), and its main principle is to use different biomacromolecules to have different hydrodynamic volumes, and achieve the purpose of separation through a gel (gel void) with a certain pore size. Take the determination of blood type by using the microcolumn gel method as an example. In the microcolumn tube, microsphere gel is injected as a filler, and the gap between the microspheres forms a specific and uniform pore size, formin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24C08J3/075C08L5/02
Inventor 刘强廖南山陈琼娣黄志强
Owner 南雄阳普医疗科技有限公司
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