A method for identification of blast resistance of indoor rice varieties

Abstract

The invention discloses a method for identifying indoor blast resistance of rice varieties. Firstly, spray inoculation is carried out indoors. In the case of the susceptible control Lijiang Xintuan Heigu onset, the varieties showing the sensitivity are graded and recorded and the test is completed; Re-raise seedlings for disease-resistant varieties, and then carry out spot inoculation on isolated leaf segments indoors. Record the grading of susceptible varieties and end the test; re-breed seedlings for disease-resistant varieties, then perform spot inoculation, and record them in time after the disease is stable. Incidence. This set of resistance screening technology combined with three inoculation methods not only avoids the deficiency of a single inoculation method, but also saves manpower and material resources, and finally can truly show the indoor blast resistance of a rice variety. The test results can be It lays a stable and reliable foundation for field promotion of varieties and excavation of variety resistance genes.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for identifying blast resistance of indoor rice varieties. Background technique [0002] Rice is an important food crop in the world, especially in Asia, and the rice blast (Magnaporthe oryzae) caused by Ascomycetes is one of the main diseases that affect the safe production of rice. In addition, the actual control of rice blast is complicated and widely distributed. Therefore, rice blast has always been paid close attention to by local governments and researchers. [0003] Hubei Province is located in the middle and lower reaches of the Yangtze River, with complex landforms, including mountains, hills and flat lakes. Rice has always been the crop with the largest planting area and total output in Hubei Province. The sown area accounts for more than 50% of the province's total grain sown area, the total output accounts for about 70%, and the commodity vo...

AI Technical Summary

Problems solved by technology

The use of disease-resistant varieties to resist the prevalence of rice blast is an effective safety measure, but in actual production, the resistance of a single disease-resistant variety will be lost after long-term large-scale planting, resulting in the prevalence and outbreak of rice blast. It is a long-term scientific research task for rice breeding and disease resistance researchers to constantly search for new resistance source materials