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Function and application of A20-binding nuclear factor inhibitory protein 3 (abin3) in the treatment of cardiac hypertrophy

A technology for inhibiting protein and cardiac hypertrophy, applied in the field of gene function and application, can solve the problem of unclear what role ABIN-3 has, and achieve the effect of protecting cardiac function

Active Publication Date: 2018-12-14
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although structurally similar to the other two members of the ABIN family, ABIN-1 and ABIN-2, it is still unclear what role ABIN-3 plays in cell life activities and diseases

Method used

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  • Function and application of A20-binding nuclear factor inhibitory protein 3 (abin3) in the treatment of cardiac hypertrophy
  • Function and application of A20-binding nuclear factor inhibitory protein 3 (abin3) in the treatment of cardiac hypertrophy
  • Function and application of A20-binding nuclear factor inhibitory protein 3 (abin3) in the treatment of cardiac hypertrophy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Establishment of mouse cardiac hypertrophy model

[0059] Aortic arch constriction (AB) was used to establish a mouse model of myocardial hypertrophy, and the operation procedure of the model was as follows:

[0060] 1.1 Animal selection

[0061] Wild-type mice (WT), ABIN3 knockout (ABIN3-KO) mice, heart-specific ABIN3 transgenic mice (ABIN3-TG) and non-transgenic mice aged 8-10 weeks and weighing 23.5-27.5 g were selected. Rats (NTG) were divided into sham operation group (sham) and AB operation myocardial hypertrophy model group, namely WT sham group, WT AB group, ABIN3-KO sham group, ABIN3-KO AB group, ABIN3-TG sham group, ABIN3-TG AB group, NTG sham group, NTG AB group, 10 mice in each group.

[0062] 1.2 Preoperative preparation

[0063] (1) Anesthesia: First weigh the mice, calculate the required amount of anesthetic (3% pentobarbital sodium) according to 90 mg / kg body weight, inject intraperitoneally, and record the injection time point. There is no...

Embodiment 2

[0071] Example 2 Detection of myocardial hypertrophy and fibrosis in myocardial hypertrophy model mice

[0072] 1. Collect materials

[0073] (1) Preliminary work: prepare in advance a urine cup filled with 20mL of 10% formaldehyde by volume, and label it (mouse number, group, type of operation and date of collection). A petri dish filled with 10% KCl solution was placed at the sampling site. Turn on the analytical balance and set it to zero for later use. Mice were then weighed and sacrificed.

[0074] (2) Material collection: Ophthalmic curved forceps clamped the vascular pedicle below the atrial appendage, cut off the heart, and quickly placed it in 10% KCl solution by mass fraction. After the heart stops beating in the diastolic phase, place it on sterilized gauze, gently squeeze the fluid in the heart cavity, dip the surface fluid dry, weigh and record, put the heart into the corresponding urine cup, fix it for 48 hours and use it for pathological testing.

[0075] (...

Embodiment 3

[0090] Example 3 Cardiac function detection in myocardial hypertrophy model mice

[0091] Ultrasound testing of heart function

[0092] 1. Preliminary preparation

[0093] (1) Anesthesia machine preparation: first connect the oxygen cylinder and the air inlet port on the anesthesia machine, then unscrew the sealing cap of the dosing port on the anesthesia machine, quickly add isoflurane to the safety scale, and then tighten the sealing cap. Unscrew the main valve on the oxygen cylinder, adjust the knob of the flow control valve, and maintain the outlet pressure at 0.2-0.3mPa.

[0094] (2) Preparation of the mice to be tested: After the mice to be tested were quickly anesthetized with isoflurane, the hair on the left chest area was shaved, and the head of the treated mice was inserted into the anesthetic catheter sleeve, and treated with 1.5-2.0% isoflurane Alkane maintains a stable anesthesia in mice.

[0095] 2. Cardiac function test

[0096] The mice were placed in the l...

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Abstract

The invention discloses a function and application of ABIN3 (A20-binding inhibitors of NF-kB 3) in treating myocardial hypertrophy and belongs to the field of gene functions and applications. A correlation between expression of the ABIN3 and myocardial hypertrophy is determined. Research results show that the inhibition of ABIN3 expression promotes myocardial hypertrophy and myocardial fibrosis and worsens the cardiac function, while the promotion of ABIN3 over-expression significantly inhibits myocardial hypertrophy and myocardial fibrosis and protects the cardiac function. Therefore, the ABIN3 may serve as a target gene used for both the screening and preparation of drugs for protecting the cardiac function, resisting myocardial fibrosis and / or preventing, relieving and / or treating myocardial hypertrophy, and an effective way to treat myocardial hypertrophy is provided.

Description

technical field [0001] The invention belongs to the field of gene function and application, and particularly relates to the function and application of A20-Binding Inhibitors of NF-κB 3 (ABIN-3) in the treatment of myocardial hypertrophy. Background technique [0002] Myocardial hypertrophy is an adaptive compensatory response of myocardial cells to the stimulation of neurohumoral factors and changes in mechanical stress load. It is a complex and dynamic process that incorporates many factors involved in regulation. Cardiomyopathy, cardiomyopathy and other common pathological processes that need to be experienced in the development of most cardiovascular diseases[1]. Myocardial hypertrophy is an adaptive compensatory response of the heart to various cardiovascular stimuli such as hemodynamic load, angiotensin, growth factors, and hormones, which can reduce the pressure on the ventricular wall and maintain or even increase cardiac output. However, long-term stress will lead ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K48/00A61K45/00A61P9/00G01N33/68
Inventor 李红良王丕晓张晓晶姬燕晓梅芳华
Owner WUHAN UNIV
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