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Defatted crab shell antioxidative polypeptide

A technology of antioxidant peptides and antioxidant peptides, applied in the direction of peptides, etc., can solve problems such as no reports, and achieve good scavenging effect and good lipid peroxidation inhibition effect.

Active Publication Date: 2015-12-30
北京姿美堂生物技术股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The applicant found that using crab shell leftovers as raw materials, the process research on the preparation of degreased crab shell antioxidant polypeptides by enzymatic hydrolysis technology is in a blank stage, and the preparation of high activity antioxidant peptides and its application by using crab shell leftovers as materials is unreported

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  • Defatted crab shell antioxidative polypeptide
  • Defatted crab shell antioxidative polypeptide
  • Defatted crab shell antioxidative polypeptide

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Embodiment Construction

[0015] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0016] A preparation method of defatted crab shell antioxidant polypeptide, the preparation process is as follows: crab shell scraps-defatting-enzymolysis-enzymolyzate-ultrafiltration-macroporous resin purification-anion exchange chromatography-gel filtration chromatography- Reverse-Phase High Performance Liquid Chromatographic Preparation - Antioxidant Peptides.

[0017] The specific steps are:

[0018] 1) Pretreatment of crab shell scraps: Portunus trituberculatus taken through a 100-mesh sieve ( Portunus trituberculatus ) crab shell scraps, add isopropanol according to the solid-to-liquid ratio of 1g:8mL, degrease at 45°C for 8h, then centrifuge at 9000rpm for 10min to remove isopropanol, and collect the solid matter of defatted crab shell scraps.

[0019] ) Enzymolysis of crab shell leftovers: Add the solids of the above defatted cra...

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Abstract

The invention discloses a defatted crab shell antioxidative polypeptide. Particularly, crab shell offals are used as a raw material, an enzymatic hydrolysate is prepared through defatting as well as neutral protease and papayotin enzymolysis, and the enzymatic hydrolysate is subjected to ultrafiltration, macroporous resin purification, anion exchange resin chromatography, gel column chromatography and reversed-phase high-performance liquid chromatography separation and purification, so as to obtain the antioxidative polypeptide Ile-Trp-Met-Glu-Cys-Asn-Trp, wherein the ESI / MS detection molecular weight is 979.15 Da. The prepared antioxidative polypeptide has an excellent scavenging effect on DPPH free radicals, hydroxyl radicals and superoxide anion free radicals; meanwhile Ile-Trp-Met-Glu-Cys-Asn-Trp also shows the excellent lipid peroxidation inhibiting effect.

Description

technical field [0001] The invention relates to a protein antioxidant peptide, in particular to a defatted crab shell antioxidant polypeptide. Background technique [0002] The output of aquatic products in China accounts for about one-third of the world's total output, but the processing rate is about 30%, which is far behind the 80% in the United States and other countries. The processing and comprehensive utilization technologies of aquatic products need to be further improved. At present, the utilization rate of by-products of aquatic product processing in my country is relatively low, while foreign countries have achieved effective classification and utilization according to the chemical composition and biochemical characteristics of by-products, and the processing output value is even several to dozens of times higher than that of fish. During the processing of shrimp and crab aquatic products, a large amount of leftovers such as shrimp heads, shrimp shells, crab shell...

Claims

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Application Information

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IPC IPC(8): C07K7/06
Inventor 曲有乐杨立业徐银峰王斌陈荫孙坤来
Owner 北京姿美堂生物技术股份有限公司