Complex microbial inoculant for simultaneously removing aniline and ammonia nitrogen as well as preparation method and application of complex microbial inoculant
A technology of compound bacterial agent and aniline degrading bacteria, which is applied in the field of ammonia nitrogen compound bacterial agent and its preparation, can solve the problems of water eutrophication, threat to ecological environment and human health, etc.
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specific Embodiment approach 1
[0024] A compound bacterial agent for simultaneously removing aniline and ammonia nitrogen is implemented, which is composed of aniline-degrading bacteria and heterotrophic nitrifying bacteria.
[0025] Aniline-degrading bacteria, belonging to the genus Pseudomonas (Pseudomonas), named: Pseudomonassp.Z1; deposit registration number: CGMCCNo.11444; depository institution: General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee; deposit The time is: September 24, 2015.
[0026] Heterotrophic nitrifying bacteria, belonging to the genus Acinetobacter (Acinetobacter), named after it: Acinetobactersp.Y1; the deposit registration number is: CGMCCNo.6563; The time is: September 14, 2012.
[0027] Wherein, the ratio of the bacterial liquid volumes of Pseudomonas Z1 and Acinetobacter Y1 is 1:1.
specific Embodiment approach 2
[0028] Implement a kind of preparation method that is used for the composite bacterial agent that removes aniline and ammonia nitrogen simultaneously described in the above-mentioned specific embodiment 1, and this preparation method is carried out according to the following steps:
[0029] (1) Preparation method of Pseudomonas Z1
[0030] Pick a small amount of bacteria from the slant stored at 4°C and inoculate it into 100mL liquid medium, shake and culture at 120rpm and 30°C for 18h, until the bacterial suspension OD 600 When the value is 0.6 to 0.8, the culture is stopped to obtain the Pseudomonas Z1 bacterial liquid; the medium composition of the Pseudomonas Z1 is: KH 2 PO 4 1.0g / L; K 2 HPO 4 1.0g / L; NaCl1.0g / L; MgSO 4 0.005g / L; MnSO 4 0.0017g / L;H 3 BO 3 0.00116g / L; ZnSO 4 ·7H 2 O0.00115g / L; FeSO 4 ·7H 2 O0.003g / L; aniline 0.4g / L; pH value is 7.0-7.2;
[0031] (2) The preparation method of Acinetobacter Y1
[0032] Pick a small amount of bacteria from the sla...
specific Embodiment approach 3
[0034] Carry out a kind of application that is used for the composite bacterial agent that removes aniline and ammonia nitrogen simultaneously as described in the above-mentioned specific embodiment 1, or implement a kind of composite bacterial agent that is used for the simultaneous removal of aniline and ammonia nitrogen described in the above-mentioned specific embodiment 2 The application of the composite bacterial agent prepared by the preparation method, the method of this application is to inoculate the composite bacterial agent in the aniline wastewater with the inoculum amount of the prepared microbial composite bacterial agent according to the volume ratio of 4%, for the simultaneous removal of aniline and its aniline Ammonia nitrogen released during degradation.
[0035] Among them, inoculating the composite bacterial agent in the aniline wastewater is to inoculate Pseudomonas Z1 and Acinetobacter Y1 in the aniline wastewater at a volume ratio of 1:1. The composite b...
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