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Newcastle disease and avian influenza combined inactivated vaccine and preparation method thereof

A dual inactivated vaccine and Newcastle disease technology, which is applied in the field of vaccine preparation, can solve the problems that immunized chickens cannot produce protective immune antibodies in time, the release speed of antigenic components is slow, and Newcastle disease has not been seen, so as to prevent Newcastle disease and H9N2 subtype Effects of Avian Influenza

Inactive Publication Date: 2016-01-06
乾元浩生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, both Newcastle disease and avian influenza dual-inactivated vaccines are prepared with mineral oil adjuvant. The inactivated antigen in the preparation is wrapped in mineral oil. After the vaccine is immunized, the antigenic components are released slowly in the body, and the immunized chickens cannot be protected in time. Sexual immune antibodies, Newcastle disease and H9N2 subtype avian influenza have a long blank period of immunity, which can easily lead to the occurrence of the two diseases
[0005] So far, there is no water-soluble preparation of chicken Newcastle disease and avian influenza dual inactivated vaccine prepared with Gel01ST adjuvant

Method used

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Examples

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Effect test

Embodiment 1

[0023] Embodiment 1 chicken Newcastle disease, avian influenza dual inactivated vaccine and preparation method thereof

[0024] Include the following steps:

[0025] 1. Preparation of inactivated antigen of chicken Newcastle disease virus LaSota strain: inoculate 10-11 day-old chicken embryos with the virus seed of chicken Newcastle disease LaSota strain, and the inoculation dose is 1000EID 50 , cultured at 36-37°C for 96-120 hours, harvested chicken embryo fluid, added formaldehyde solution to it, so that the final concentration of formaldehyde reached 0.2%, inactivated at 37°C for 24 hours after mixing, passed the sterility test and inactivation test Afterwards, store at 2-8°C for later use.

[0026] 2. Preparation of inactivated antigen of H9N2 subtype avian influenza virus WF strain: Inoculate 10-11-day-old chicken embryos with the virus seed of H9N2 subtype avian influenza WF strain, and the inoculation dose is 1000EID 50 , cultured at 36-37°C for 72-96 hours, harvested...

Embodiment 2

[0032] The application of embodiment 2 chicken Newcastle disease, avian influenza dual combination inactivated vaccine

[0033] The Newcastle disease and avian influenza dual inactivated vaccine prepared in Example 1 were used to immunize 10 28-day-old SPF chickens according to a dose of 20 microliters per chicken, and blood was collected to separate serum at 7, 14, and 28 days after immunization, and the serum was detected. Calculate the average value of HI antibody for Newcastle disease HI antibody in Chinese chickens. The average value of HI antibody reached 1:6.96 on 7 days after immunization; 1:18.38 on 14 days; 1:90.51 on 21 days. During the test period, no immune adverse reactions were observed in the test chickens. At the end of the experiment, the test chicken was autopsyed, and no pathological changes were observed.

Embodiment 3

[0034] Embodiment 3 Chicken Newcastle disease, the application of avian influenza dual inactivated vaccine

[0035] The chicken Newcastle disease, avian influenza dual inactivated vaccine prepared in Example 1 was immunized according to the dosage of 0.3 milliliters per chicken, and 10 28-day-old SPF chickens were immunized, and blood was collected and separated serum was collected at 7, 14, and 28 days after immunization, and the serum was detected. Chicken Newcastle disease HI antibody, calculate the average value of HI antibody. The average value of HI antibody reached 1:11.31 at 7 days after immunization; 1:157.59 at 14 days; and 1:3565.78 at 21 days. During the test period, no immune adverse reactions were observed in the test chickens. At the end of the experiment, the test chicken was autopsyed, and no pathological changes were observed.

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Abstract

The invention provides a Newcastle disease and avian influenza combined inactivated vaccine which comprises a Newcastle disease virus LaSota strain inactivated antigen, an H9N2 subtype avian influenza virus WF strain activated antigen, a water-soluble vaccine adjuvant G2101ST and a PBS solution of 6.8-7.2 in pH. The invention further provides a preparation method of the inactivated vaccine. The preparation method includes: preparing and purifying the Newcastle disease virus LaSota strain inactivated antigen and the H9N2 subtype avian influenza virus WF strain activated antigen; preparing the combined inactivated vaccine. The combined inactivated vaccine is a water-soluble preparation, can quickly generate immunity after being injected and can effectively prevent both Newcastle disease and H9N2 subtype avian influenza.

Description

technical field [0001] The invention relates to the field of vaccine preparation, in particular to a dual inactivated vaccine for Newcastle disease and bird flu and a preparation method thereof. Background technique [0002] Newcastle disease and H9N2 subtype avian influenza are two major viral infectious diseases of chickens, which can cause disease in chickens of various ages. Respiratory symptoms of varying degrees occur in mild cases of affected chickens, leading to a decline in production performance, and severe cases may cause the death of the entire flock, causing serious economic losses and triggering a biosafety crisis. [0003] Vaccine prevention is an effective way to prevent and control the two diseases. The dual inactivated vaccine containing two inactivated antigens of Newcastle disease and H9N2 subtype avian influenza can prevent the two diseases at the same time through immunization. [0004] At present, both Newcastle disease and avian influenza dual-inacti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61P31/14A61P31/16
Inventor 王贺民刘兆环张贺楠李浩鹏陈秋阁周蕾蕾
Owner 乾元浩生物股份有限公司
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