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A kind of Avian bacillus paragallinarum bacterial strain and application thereof

A technology of poultry and bacilli, which is applied in the direction of bacteria, antibacterial drugs, and veterinary vaccines, can solve the problems of vaccine immune protection failure, achieve good immune protection, reduce production costs, and improve the effect of protection

Active Publication Date: 2021-03-12
PULIKE BIOLOGICAL ENG INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the prevailing serotype of chicken infectious rhinitis in China is mainly type A, in recent years, there have been reports of type B and type C in many areas, especially the isolation rate of type B pathogenic bacteria is as high as 50%, and the existing vaccines often have immune protection failure phenomenon

Method used

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  • A kind of Avian bacillus paragallinarum bacterial strain and application thereof
  • A kind of Avian bacillus paragallinarum bacterial strain and application thereof
  • A kind of Avian bacillus paragallinarum bacterial strain and application thereof

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Experimental program
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Embodiment approach

[0023] As an embodiment of the present invention, in the vaccine composition of the present invention, the antigen content of the inactivated whole bacterium of Avibacterium paragallinarum type B HN5 strain is 10% before inactivation. 7.0 CFU / ml~10 9.0 CFU / ml.

[0024] As a preferred embodiment of the present invention, in the vaccine composition of the present invention, the antigen content of the inactivated whole bacteria of Avibacterium paragallinarum type B HN5 strain is 3×10 before inactivation. 7.0 CFU / ml~10 8.0 CFU / ml.

[0025] In the vaccine composition of the present invention, the content range of the inactivated whole bacterium antigen of the Avibacterium paragallinarum type B HN5 strain can also be 10 7.0 CFU / ml~3×10 7.0 CFU / ml, or 10 7.0 ~10 8.0 CFU / ml, or 3×10 7.0 CFU / ml~10 9.0 CFU / ml, or 10 8.0 ~10 9.0 CFU / ml.

[0026] As an embodiment of the present invention, in the vaccine composition of the present invention, the pharmaceutically acceptable carri...

Embodiment 1

[0058] Example 1 Isolation and Identification of Avibacterium paragallinarum Type B HN5 Strain

[0059] 1. Source of disease material

[0060] Chicken farms that were normally immunized with the trivalent inactivated vaccine of Avian bacillus paragallinarum had a significant drop in egg production rate, accompanied by symptoms such as facial swelling, runny nose and conjunctivitis in some chickens. Emergent treatment with antibiotics followed, with no significant improvement in symptoms. In order to further find out the reason, select the chickens whose egg production dropped significantly to isolate the pathogen.

[0061] 2. Isolation and cultivation of strains

[0062] Execute the sick chicken, cut off the chicken head, put it in the ultra-clean table, burn the outer surface of the infraorbital sinus with a red-hot blade, incise the infraorbital sinus with a sterile blade, and take out the infraorbital sinus with a sterile cotton swab Contents, streaked on a chicken broth a...

Embodiment 2

[0082] The proliferation of embodiment 2 Avian bacillus paragallinarum type B HN5 strain bacterium liquid

[0083] 1. Primary seed propagation

[0084] Streak and inoculate the strains on the chicken broth agar plate, in the presence of 5% to 10% CO 2 After culturing at 37°C for 18-24 hours in the environment, pick typical colonies and inoculate them on chicken broth agar slant, at 37°C, 5% CO 2 Cultivate for 24 hours, store at 2-8°C, and use it as a first-class seed after passing the pure inspection.

[0085] 2. Secondary seed propagation

[0086] Take the primary seeds and inoculate them in the chicken broth medium, place them on a shaker at 37°C, and cultivate them at 180r / min for 10-12 hours, as the secondary seeds.

[0087] 3. Semi-synthetic medium formula

[0088] Take 5g of polyvalent peptone, 5g of casein peptone, 5g of sodium glutamate, 5g of sodium chloride, 3g of yeast powder, add 1000ml of distilled water to fully dissolve, adjust the pH value to 7.2-7.4 with 2...

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Abstract

The invention relates to the Avian bacillus paragallinarum type B HN5 strain and the prepared vaccine composition and application thereof. The Avibacterium paragallinarum type B HN5 strain has good immunogenicity, and the single and combined vaccines prepared by it can effectively prevent the wild strains that are currently clinically prevalent, and have better immune efficacy than commercial vaccines in the prior art.

Description

technical field [0001] The invention belongs to the field of veterinary medicine, and in particular relates to a strain of Avian bacillus paragallinarum and the prepared vaccine composition and application thereof. Background technique [0002] Avibacterium paragallinarum (Avibacterium paragallinarum) is a pathogenic bacterium that causes acute upper respiratory tract disease in chickens, and is divided into three serotypes: A, B, and C. The disease was first discovered in Poland and the United States, and later frequently occurred in other countries. Since 1980, there have been frequent reports of this disease in my country, mainly of type A and type C. Commonly known as chicken infectious rhinitis, this respiratory disease is clinically characterized by facial edema, sinusitis, and tearing. It can cause a decrease in egg production of laying hens, hindered growth of reared chickens, delayed laying period, and decreased weight gain of broiler chickens. Cause huge economic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K39/102A61K39/295A61K39/17A61P31/04A61P31/14C12R1/01
CPCA61K39/102A61K39/12A61K2039/521A61K2039/552A61K2039/55505A61K2039/70C12N2760/18134C12N1/205C12R2001/01A61K2300/00
Inventor 田克恭金云云孙进忠张许科
Owner PULIKE BIOLOGICAL ENG INC
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