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Chicken infectious rhinitis vaccine composition and its preparation method and application

A technology of vaccine composition and poultry, which is applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc., which can solve the problems of complex process, low output, and poor preventive effect of diseased chickens

Active Publication Date: 2020-03-10
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the preparation of the existing chicken infectious rhinitis inactivated vaccine has complex processes, high production costs, and low yields.
Moreover, the current Avianbacterium paragallinarum vaccine is less effective in preventing some diseased chickens

Method used

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  • Chicken infectious rhinitis vaccine composition and its preparation method and application
  • Chicken infectious rhinitis vaccine composition and its preparation method and application
  • Chicken infectious rhinitis vaccine composition and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The selection of embodiment 1 Avian bacillus paragallinarum bacterial classification

[0056] 1. The source of the strain

[0057] Paragallinarum type A HN3 strain and Avibacterium paragallinarum type C SD3 strain were isolated and identified by the laboratory.

[0058] 2. Cultivate traits

[0059] Cross-streaked with Staphylococcus aureus on NADH-free blood agar plate, in the presence of 5% to 10% CO 2 Under certain conditions, cultured at 37°C for 24 hours, satellite colonies will grow around Staphylococcus.

[0060] Inoculate on chicken broth agar plate in 5%-10% CO 2 Under the conditions of 37°C for 24 hours, a round, smooth, off-white, translucent dewdrop-like colony with a diameter of about 0.3mm is formed, which has a strong fluorescence when observed with a low-power lens at 45° refraction.

[0061] 3. Virulence

[0062] The 12-hour chicken broth culture of Avibacterium paragallinarum HN3 strain and SD3 strain was made 10 times with PBS (0.01mol / L, pH value...

Embodiment 2

[0063] The proliferation of embodiment 2 Avian bacillus paragallinarum bacterium liquid

[0064] 1. Primary seed propagation

[0065] Streak and inoculate the strains on the chicken broth agar plate, in the presence of 5% to 10% CO 2 After culturing at 37°C for 18-24 hours in the environment, select 5 typical colonies that meet the standards and inoculate them in the yolk of 6- to 7-day-old chicken embryos, continue to incubate at 37°C, collect the yolk fluid of chicken embryos that died within 30 hours, and purify After passing the inspection, it will be regarded as a first-class seed. Storage below 20°C should not exceed 1 month, otherwise it will be rejuvenated through chicken embryos, but it should not exceed 5 generations.

[0066] 2. Secondary seed propagation

[0067] Take the egg yolk fluid of infected chicken embryos, streak and inoculate the chicken broth agar plate, and inoculate it in the environment containing 5% to 10% CO 2 Cultivate in the environment at 37°...

Embodiment 3

[0072] The preparation of embodiment 3 chicken infectious rhinitis inactivated vaccines

[0073] 1. Bacterial liquid inactivation and inspection

[0074] The bacterial liquid of A. paragallinarum type A HN3 strain and the bacterial liquid of Avian bacteria paragallinarum type C type SD3 strain prepared in Example 2 were respectively added with a final concentration of 0.01% thimerosal according to the amount of the bacterial liquid, and inactivated at 2 to 8° C. for 5 days. Use 2 sticks of chicken broth slant, inoculate 0.2ml each, and culture at 37°C for 7 days, all of which grow aseptically.

[0075] 2. Adjuvant Sterilization

[0076] Take aluminum hydroxide gel adjuvant, autoclave at 121°C for 30 minutes, and cool to room temperature for later use.

[0077] 3. Matching seedlings

[0078] Dilute type A antigen and type C antigen with PBS of pH 7.2 according to the results of live bacteria count before inactivation, mix type A and type C antigen in proportion, mix with adj...

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Abstract

The invention provides a chicken infectious rhinitis vaccine composition, wherein, the vaccine composition contains immune amount of Avian bacillus paragallinarum type A antigen and / or Avian bacilli paragallinarum type C antigen and a carrier. The invention also provides the preparation method and application of the vaccine composition. The immune effect of the vaccine composition of the invention is better than that of the vaccine prepared by the existing bacterial strains, the antigen content can be greatly reduced, the immune effect is still good, and the production cost is greatly reduced.

Description

technical field [0001] The invention relates to avian bacillus paragallinarum, a vaccine composition of the avian bacilli paragallinarum and a preparation method and application thereof, belonging to the field of biological products. Background technique [0002] Haemophilus paragallinarum (Haemophilus paragallinarum) is a pathogenic bacterium that causes acute upper respiratory tract disease in chickens. It is divided into three serotypes: A, B, and C. The disease was first discovered in Poland and the United States, and later frequently occurred in other countries. Since 1980, there have been frequent reports of this disease in my country, mainly of type A and type C. Commonly known as chicken infectious rhinitis, this respiratory disease is clinically characterized by facial edema, sinusitis, and tearing. It can cause a decrease in egg production of laying hens, hindered growth of reared chickens, delayed laying period, and decreased weight gain of broiler chickens. Cau...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/02A61K39/116A61K39/145A61P31/04A61P11/02A61P31/14
Inventor 张许科孙进忠陈雯雯田克恭
Owner PU LIKE BIO ENG
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