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Preparation method and use method of immune factor for preventing pathopoiesis of aquaculture animal

An immune factor and aquaculture technology, applied in the field of bioengineering, can solve long-term problems such as months or even years, and narrow practical applications, so as to achieve simple and feasible operation, low price, and ease the death of farmed animals Effect

Inactive Publication Date: 2021-06-01
威海长青海洋科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, a more feasible method is vaccine immunization, but the practical application of this type of immunization is relatively narrow, and generally only targets individual pathogenic bacteria, such as chicken plague and rabbit plague of large organisms; moreover, the acquisition cycle of vaccines is long, and generally requires A few months or even a few years cannot solve the problem of rapid death of aquatic animals; in addition, vaccines need to be immunized by artificial injection, which is not suitable for small-sized aquaculture organisms such as abalone, shrimp, crab, and clams.

Method used

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  • Preparation method and use method of immune factor for preventing pathopoiesis of aquaculture animal
  • Preparation method and use method of immune factor for preventing pathopoiesis of aquaculture animal
  • Preparation method and use method of immune factor for preventing pathopoiesis of aquaculture animal

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Embodiment Construction

[0025] The specific preparation method and application method of the immune factor provided by the present invention will be described in detail below with reference to the accompanying drawings and examples.

[0026] In this example, to simulate Vibrio vulnificus Vibrio vulnificus The immune factor prepared for the death of juvenile abalone and the immunization process using the immune factor are illustrated as examples.

[0027] 1. Scenario simulation: take juvenile abalones with an average size of about 3cm, and place them in running water for one week. The running water comes from seawater after sand filtration, and feed appropriate amount of fresh kelp every afternoon; cultivate Vibrio vulnificus Vibrio vulnificus to the logarithmic phase, and then according to the final concentration of 10 3-5 The final concentration of cells / mL was added to the water body of juvenile abalone after temporary breeding, and the addition continued for 3 days; about 10 days later, juvenil...

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Abstract

The invention relates to the field of bioengineering, in particular to a preparation method and a use method of an immune factor for preventing pathopoiesis of aquaculture animals. According to the technical scheme, the preparation method and the use method of the immune factor for preventing pathopoiesis of aquaculture animals comprise the following steps: (1) preparing a pathogenic bacterium culture medium; and (2) preparing a immune factor. When the immune factor is used, animals are immunized in a mixing mode or a direct spreading mode. Most of effective components are simple culture medium components, part of the effective components are derived from the environment of a livestock farm, cultured organisms and the environment cannot be influenced, and the immune factor is non-toxic, harmless, safe, environmentally friendly, low in price, simple, feasible and capable of achieving large-scale production.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for preparing and using an immune factor for preventing aquaculture animals from causing disease. Background technique [0002] Aquaculture is one of the important agricultural activities in our country. It can provide high-quality food for the general public and provide a large amount of raw materials for industrial development. However, pathogenic bacterial diseases have become one of the bottlenecks for the rapid development of aquaculture in recent years. At present, diseases caused by microorganisms involve almost all aquaculture species. In 2017 alone, the economic losses caused by microbial diseases were as high as 36 billion. Among them, vibriosis has become the most important stumbling block of aquaculture diseases, even reaching the level of "talking about turning pale". Pathogenic bacteria have always existed in aquaculture. Generally, the concentration of path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/28A61K38/16A61P31/04A23K50/80A23K20/147
CPCC07K14/28A61P31/04A23K50/80A23K20/147A61K38/00Y02A40/70
Inventor 卢龙飞常丽荣杨晓斌李长青肖露阳闫欣
Owner 威海长青海洋科技股份有限公司
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