Agaricus rubellus polysaccharide and application thereof
A technology of purple mushroom and polysaccharide, applied in the field of purple mushroom polysaccharide and its application
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Embodiment 1
[0034]Example 1: Weigh 50g of purple mushrooms, crush them, add 10 times the volume of water, ultrasonically extract for 2 hours, repeat 2 times, combine the filtrate after filtration, spin the filtrate to concentrate, add 75% ethanol to the concentrate to precipitate, filter Finally, the filter residue was washed successively with 95% ethanol and absolute ethanol (it could also be washed successively with 85% ethanol, 95% ethanol, absolute ethanol, acetone, ether), and vacuum-dried to obtain 10.29 g of polysaccharide.
Embodiment 2
[0035] Example 2: Weigh 50g of purple mushrooms, crush them, add 10 times the volume of water, decoct and extract twice, each time for 2 hours, filter and combine the filtrate, concentrate the filtrate after removing the solvent, add ethanol for precipitation, and use 95% The precipitate was washed with ethanol several times (it can also be washed with absolute ethanol, or washed sequentially with 85% ethanol, 95% ethanol, absolute ethanol, acetone, and ether) to obtain 13.58 g of purple mushroom polysaccharide.
Embodiment 3
[0036] Example 3: Weigh 50g of purple mushrooms, crush them, add 20 times the volume of water, ultrasonically extract twice, each time for 2 hours, filter and combine the filtrate, concentrate the filtrate after removing the solvent, add ethanol for precipitation, and use 95% ethanol The precipitate was washed several times (it can also be washed with absolute ethanol, or washed sequentially with 85% ethanol, 95% ethanol, absolute ethanol, acetone, and ether) to obtain 12.17 g of purple mushroom polysaccharide.
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