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Method of detecting peroxide value of liquid-phase edible oil

A technology for peroxide value and edible oil, which is applied in the measurement of scattering characteristics, color/spectral characteristics, etc., can solve the problems of cumbersome steps, difficult census use, and high reagent cost

Inactive Publication Date: 2016-01-13
马井芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

High-efficiency liquid chromatography requires the use of large-scale instruments, and the cost of reagents is high, so it is difficult to use as a general survey; while the reaction time of chemical titration is long, the steps are cumbersome, the reagents need to be prepared more than one week in advance, and all manual operations are easily interfered by human factors.

Method used

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specific Embodiment approach 1

[0023] DETAILED DESCRIPTION 1. 200 grams of citric acid buffer reagent, 30000 units of horseradish peroxidase and 30 grams of stabilizer were dissolved in 1000 milliliters of deionized water successively. Immerse the nylon 66 microporous filter membrane in the solution, take it out after 20 seconds, and dry it at 60°C; then dissolve 10 grams of color developer and 20 grams of reaction accelerator in 1000 ml of absolute ethanol, and soak the filter membrane in the solution After 29 seconds, take it out and dry it at 60°C, cut it into circular membranes with a diameter of 10 mm, and pack them in airtight vacuum-packed aluminum foil bags for use.

[0024] 2. Use a pipette to drop a drop of the oil sample to be tested on the drug-containing diaphragm, and measure the reflectance of the diaphragm at a wavelength of 630 nm after 120 seconds, and calculate the peroxide value of the sample according to the standard equation or relationship curve.

[0025] 3. Immerse the diaphragm in t...

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Abstract

A method of detecting peroxide value of liquid-phase edible oil is quantitatively carried out through combination of a drug-containing film sheet with a visible light analyzer.

Description

technical field [0001] The invention relates to a method for quickly measuring the peroxide value of liquid edible oil. Background technique [0002] The detection of peroxide value of edible oil is a key means to ensure the quality of oil products. The widely used determination methods at home and abroad mainly include high performance liquid chromatography and chemical titration. The HPLC method requires the use of large instruments, and the cost of reagents is high, so it is difficult to use as a general survey. The chemical titration method has a long reaction time and cumbersome steps. The reagents need to be prepared more than one week in advance, and all manual operations are easily interfered by human factors. At present, each epidemic prevention department in my country detects the peroxide value of edible oil in accordance with the analysis method (chemical titration method) stipulated in GB / T5009.37-1996. [0003] The purpose of this invention is to propose a det...

Claims

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Application Information

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IPC IPC(8): G01N21/55G01N21/29
Inventor 马井芳
Owner 马井芳