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Micro-channel electrophoretic quantitative analysis device and method

A quantitative analysis, microchannel technology, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of cumbersome methods, inability to separate and analyze biomolecules, and unfavorable long-term development.

Inactive Publication Date: 2016-01-13
窦晓鸣 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the existing electrophoresis detection system cannot accurately separate and analyze biomolecules such as DNA, RNA, and protein, and the method is cumbersome, which is not conducive to long-term development

Method used

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  • Micro-channel electrophoretic quantitative analysis device and method
  • Micro-channel electrophoretic quantitative analysis device and method

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Embodiment Construction

[0069] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific diagrams.

[0070] refer to figure 1 , figure 2 , a microchannel electrophoresis quantitative analysis device, comprising an electrophoretic detection system, characterized in that the electrophoretic detection system includes a mixture to be analyzed, a microchannel and a fluorescence detection device, and the mixture to be analyzed includes a standard reference and a group to be analyzed The microchannel is provided with a positive terminal and a negative terminal, and the cross-sectional diameter of the microchannel is on the micron scale, and the microchannel is filled with an electrolyte; the mixture to be analyzed is located in the negative terminal, and the microchannel A detection area is also provided in the channel, and the fluorescence detection dev...

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Abstract

The invention relates to biological macromolecular detection and specifically relates to electrophoretic analysis detection. A micro-channel electrophoretic quantitative analysis device comprises an electrophoretic detection system; the electrophoretic detection system comprises a mixture to be analyzed, a micro channel, and a fluorescence detection device, the mixture to be analyzed comprises a standard reference and a component to be analyzed; the micro channel is provided with a positive terminal and a negative terminal; the diameter of cross section of the micro channel is in the millimeter level, the micro channel is full of electrolyte; the mixture to be analyzed is arranged in the negative terminal, a detection area is arranged in the micro channel, and the fluorescence detection device faces the detection area. According to the technical scheme, the structure of micro-channel polymer electrophoretic system is optimized; and the micro channel can separate and analyze the biological macromolecular substances. The micro channel can be used to separate the mixture to be analyzed. Electrolyte can separate the mixture to be analyzed according to the different molecular weights during the electrophoresis process.

Description

technical field [0001] The invention relates to the detection of biological macromolecules, in particular to electrophoresis analysis and detection. Background technique [0002] In the process of analyzing substances, we often use electrophoresis to separate the required substances. Electrophoresis has been widely used in analytical chemistry, biochemistry, clinical chemistry, toxicology, pharmacology, immunology, microbiology, food chemistry, etc. field. When media such as filter paper and polyacrylamide gel were successively introduced into electrophoresis, electrophoresis technology has developed rapidly. The rich and colorful electrophoretic forms make it widely used. [0003] However, the existing electrophoresis detection system cannot accurately separate and analyze biomolecules such as DNA, RNA, and protein, and the method is cumbersome, which is not conducive to long-term development. Contents of the invention [0004] The purpose of the present invention is t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 刘晨晨窦晓鸣山口佳则倪一
Owner 窦晓鸣