An Analytical Method for the Simultaneous Resolution of Chiral Molecules Specific to Tobacco Nitrosamines
A technology of chiral molecules and nitrosamines, which is applied in the field of tobacco analytical chemistry to achieve good resolution and high accuracy
Inactive Publication Date: 2017-04-19
GUIZHOU TOBACCO SCI RES INST
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Problems solved by technology
[0006] The technical problem to be solved by the present invention is to provide a simple and easy-to-operate analysis method for the defects of the existing analysis methods for splitting tobacco-specific nitrosamine chiral molecules. This method does not need to use high performance liquid chromatography to separate NNN and Other tobacco-specific nitrosamines, the total time of sample pretreatment and analysis is reduced, the loss of samples in the pretreatment process is reduced, and the resolution effect is better than the currently reported analytical methods of capillary electrophoresis and gas chromatography thermal energy analyzer
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[0026] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings.
[0027] refer to figure 1, before the experiment, the analytical column in the gas chromatographic thermal energy analyzer is designed as two chromatographic columns, one of which is a chiral chromatographic column 1, and Cyclosil-B column (30 m× 0.25 mm i.d., 0.25 μm film thickness , J & W Scientific, Folsom, CA), the other is a medium polar column 2, Zebron ZB-5column (30 m × 0.53 mm i.d., 1.5 μm film thickness, Phenomenex, Torrance, CA) or AgilentDB- 5 MS (30m × 0.25mm × 0.25µm). The chiral chromatographic column 1 and the medium polar chromatographic column 2 are socketed together with their bottom connectors through the column sleeve 3, and the inner cavities of the column sleeve 3 shrink inward from both ends to form a tapered cavity, which is convenient for...
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The invention discloses a method capable of simultaneously splitting characteristic nitrosamine chiral molecules of tobacco. According to the method, two analysis columns are arranged in a gas chromatography thermal energy analyzer, one is a chiral chromatographic column, and the other one is a medium polar chromatographic column. During the analysis process, the medium polar chromatographic column can separate NNN from NAB and NAT, so the step of high performance liquid chromatography (HPLC) separation can be saved. Because the HPLC separation has the disadvantages that the operation is tedious, the analysis time is long, and the sample detection limit is influenced by the sample loss, the provided method has the advantages of good splitting effect and high accuracy. The provided method can be used to analyze the chiral composition of characteristic nitrosamine of tobacco, provides reference for the research on nitrosamine composition, and provides technical support for the research on the harmfulness of different chiral molecules of NAB and NAT.
Description
technical field [0001] The invention relates to a method for analyzing tobacco-specific nitrosamine chiral molecules, belonging to the field of tobacco analytical chemistry. Background technique [0002] Tobacco-specific nitrosamines (TSNAs) are a group of carcinogenic substances. TSNAs exist in tobacco leaves and smoke, mainly including N´-nitrosonornicotine (NNN), 4-(N-methylnitrosamino)-1-(3-pyridyl)-1- Butanone (NNK), N´-nitrosobasine (NAB) and N´-nitrosoannicotine (NAT). They are mainly formed by the nitrosation reaction of tobacco alkaloids during post-harvest conditioning (Bush, L.P. et al. (2001) Formation of tobacco-specific nitrosamines in air-cured tobacco. Recent Adv. Tob. Sci.,27 , 23–46.; Djordjevic, M. V et al. (1989) Tobacco-specific nitrosamine accumulation and distribution in flue-cured tobacco alkaloid isolines. J. Agric. Food Chem., 37, 752–756.). Generally, the contents of NNN and NAT were higher than the other two contents, while NNN and NNK were mor...
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IPC IPC(8): G01N30/88G01N30/06
Inventor 蔡斌
Owner GUIZHOU TOBACCO SCI RES INST