Applications of an N-ethylmaleimide sensitive factor in preventing Japanese encephalitis virus infection

A kind of Japanese encephalitis virus, maleimide technology, applied in the field of biomedicine

Active Publication Date: 2016-01-27
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Problems solved by technology

[0007] At present, there is no report on the role of N-ethylmaleimide-sensitive factor (NSF) in JEV infection. In-depth research on this molecule can not only improve the understanding of JEV infection and pathogenic mechanism, but also contribute to the prevention of Provide new ideas and targets for the treatment of JEV infection

Method used

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  • Applications of an N-ethylmaleimide sensitive factor in preventing Japanese encephalitis virus infection
  • Applications of an N-ethylmaleimide sensitive factor in preventing Japanese encephalitis virus infection
  • Applications of an N-ethylmaleimide sensitive factor in preventing Japanese encephalitis virus infection

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Embodiment 1

[0047] 1 Design and synthesis of specific siRNA sequences for each host cell molecule.

[0048] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and commonly used software to conduct biological analysis of each target gene, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principle, and compare it with the human genome sequence through the blast function of the GeneBank database to ensure no homology; exclude potential siRNAs with 8 consecutive bases at the 5' end of the antisense chain paired with other genes; exclude any consecutive 14 bases base-pairing potential siRNAs with other genes. The design software was used for pre-evaluation and determination, and three targets with the best kinetic parameters were selected to enter the subsequent experimental process. A total of three interference sequences were synthesized for each gene, as shown in Table 1....

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Abstract

The invention relates to the biomedical technology field, and provides a new target spot for resisting Japanese encephalitis virus infection and applications. Human brain microvascular endothelial cells (HBMEC) are employed as target cells, the RNA interference technology is employed to reduce expression of target cell host proteins, a host factor capable of inhibiting Japanese encephalitis virus infection of human brain microvascular endothelial cells (HBMEC) effectively is sought, the function of protecting the blood brain barrier is achieved, viruses are prevented from going through the blood brain barrier and infecting the central nervous system. Applications of N-ethylmaleimide sensitive factor in preventing Japanese encephalitis virus infection are provided. Experiments show that the N-ethylmaleimide sensitive factor (NSF) plays an important role in inhibiting JEV infection of HBMEC, the NSF expression is reduced, and JEV infection can be inhibited obviously. Applications of NSF in preparation of medicines preventing or treating Japanese encephalitis virus infection are provided.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and is a new target and application for resisting Japanese encephalitis virus infection. Background technique [0002] Japanese encephalitis virus, also known as Japanese encephalitis virus (JEV), belongs to the Flaviviridae (Flaviviridae) genus (Flavivirus), and is a single positive-strand RNA virus with an envelope [Gubler, D., G.Kuno , and L. Markoff. Flaviviruses, p. 2007. 1153-1252. In D. M. Knipe and P. M. Howley (ed.), Fields virology, 5thed., vol. 1. Lippincott-Williams & Wilkins, Philadelphia, PA.]. JEV is the pathogen of Japanese encephalitis, which is mainly transmitted by mosquitoes. After infection, it can cause severe acute neurological symptoms such as encephalitis or meningitis, which poses a huge threat to human health. Japanese encephalitis is mainly prevalent in parts of East Asia, Southeast Asia, and Oceania. Currently, the main means of control are vaccine prevention and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P31/14
Inventor 朱勇喆戚中田徐庆强赵平陈生林
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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