Biological plugging regulator, preparation method and application
A plugging control agent and biological technology, applied in the directions of chemical instruments and methods, drilling compositions, etc., can solve the problems of direct intrusion or channeling of bacterial liquid, poor formation homogeneity, poor application effect, etc., and achieve non-degradation. , Good salt tolerance, increase the effect of sweep volume
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Embodiment 1
[0025] The biological clogging agent in this embodiment is composed of the following components in mass percentage: 10% dry powder of encapsulated strains, 10% nutrient base, 1% hydrogen peroxide, and the balance is water.
[0026] The preparation steps of the encapsulated strain dry powder are as follows:
[0027] 1) Xanthomonas campestris (purchased from China Center for Type Culture Collection, strain preservation number: CCTCCAB96030, genus name: Xanthomonas, species name: campestris, other preservation unit number: ATCC 9924, preservation date: August 1996 10 days) After activation, inoculate into conventional LB medium (or potato medium), ferment and cultivate for 36 hours to the logarithmic phase, centrifuge at a speed of 3000r / min for 20min, discard the supernatant, and take sediment and glycerol according to the volume ratio of 1 : 1 mix evenly, the mixture is first allowed to stand at a temperature of 4°C for 60min, then at a temperature of -30°C and -80°C for 90min,...
Embodiment 2
[0037] The biological clogging agent in this embodiment is composed of the following components in mass percentage: 5% of encapsulated strain dry powder, 0.4% of nutrient base, 0.1% of hydrogen peroxide, and the balance is water. The preparation method is the same as in Example 1.
Embodiment 3
[0039] The biological clogging agent in this embodiment is composed of the following components in mass percentage: 5% of encapsulated strain dry powder, 5% of nutrient base, 0.5% of hydrogen peroxide, and the balance is water. The preparation method is the same as in Example 1.
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