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A kind of preparation method of Ganoderma lucidum terpene ketone alcohol

A technology of ganoderma terpene ketols and ganoderma fruiting bodies, applied in the field of natural medicinal chemistry, can solve the problems of no large-scale preparation method of ganoderma terpene ketols, and achieve the effects of low cost, simple operation, and little pollution

Active Publication Date: 2017-06-30
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Through literature search, there is no large-scale preparation method of ganoderma lucidum terpene ketone alcohol

Method used

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  • A kind of preparation method of Ganoderma lucidum terpene ketone alcohol
  • A kind of preparation method of Ganoderma lucidum terpene ketone alcohol
  • A kind of preparation method of Ganoderma lucidum terpene ketone alcohol

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Experimental program
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Effect test

Embodiment 1

[0030] Slice 50kg of Ganoderma lucidum fruiting body, feed into the pilot extraction equipment, add 8 times the volume of 87% ethanol aqueous solution to extract twice, the first time for 2 hours, the second time for 1 hour. The extraction temperatures were all 65°C. The two extracts were combined, heated, boiled and concentrated in a cooking tank, and then heated and concentrated under reduced pressure with a 20L rotary evaporator to obtain the fruiting body extract of Ganoderma lucidum.

[0031] Pass the fruiting body extract of Ganoderma lucidum through macroporous resin D101, and use 50%, 60%, 70%, 80%, 90%, 95% ethanol to sequentially elute in gradients, and the eluents are respectively concentrated under reduced pressure, and then combined 95% and 90% % eluted components (194g in total), through normal phase silica gel column chromatography (100-200 mesh, 2200g), chloroform:methanol (100:0-7:3) gradient elution, each part collects 1000ml, collects 92 share. According t...

Embodiment 2

[0034] Slice 50kg of Ganoderma lucidum fruiting body, feed into the pilot extraction equipment, add 8 times the volume of 87% ethanol aqueous solution to extract twice, the first time for 2 hours, the second time for 1 hour. The extraction temperatures were all 65°C. The two extracts were combined, heated, boiled and concentrated in a cooking tank, and then heated and concentrated under reduced pressure with a 20L rotary evaporator to obtain the fruiting body extract of Ganoderma lucidum.

[0035] Pass the fruiting body extract of Ganoderma lucidum through macroporous resin D101, and use 50%, 60%, 70%, 80%, 90%, 95% ethanol to sequentially elute in gradients, and the eluents are respectively concentrated under reduced pressure, and then combined 95% and 90% % Elution fraction (194g), through normal phase silica gel column chromatography (100-200 mesh, 2200g), chloroform:methanol (100:0-7:3) gradient elution, each collection 1000ml, a total of 92 collections flow portion. Acc...

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Abstract

The present invention discloses a ganodermenonol preparation method, which comprises: (1) extracting and enriching ganodermenonol, wherein ganoderma lucidum fruiting body is added to an ethanol solution, heating extraction is performed, the extraction solution is concentrated, the concentrated solution is subjected to ethanol gradient elution adsorption through a macroporous resin, pressure reducing concentration is performed, and column chromatography is performed to obtain ganodermenonol-rich component; and (2) separating the ganodermenonol through a high-speed countercurrent chromatography method, wherein the ganodermenonol is separated by using a high-speed countercurrent chromatograph, analysis detection is performed by using a high performance liquid chromatography method, and collection, concentration, drying and separation are performed to obtain the ganodermenonol. The ganodermenonol preparation method of the present invention has characteristics of simple operation, low pollution, low cost, high ganodermenonol yield, and high ganodermenonol purity.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a preparation method of ganoderma lucidum terpene ketone alcohol. Background technique [0002] Ganoderma lucidum terpene ketols are triterpenoids, CAS No. 104700-97-2, molecular formula C 30 h 46 o 2 , the molecular weight is 438.685, and the molecular structure formula is as follows: [0003] [0004] Ganoderma lucidum triterpenes are secondary metabolites of Ganoderma lucidum, which have various pharmacological activities. It has been reported in the literature that Ganoderma lucidum terpene ketols have good anti-tumor, whitening, anti-inflammatory, and UV-protective effects in in vitro cell experiments. [0005] Through literature search, no large-scale preparation method of ganoderma lucidum terpene ketols has been found. Contents of the invention [0006] The object of the present invention is to provide a kind of preparation method of Ganode...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00
Inventor 冯娜张劲松田振唐庆九冯杰杨焱唐传红魏雨恬
Owner SHANGHAI ACAD OF AGRI SCI