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Application of maize mitochondrion pyruvic acid transporter gene ZmNRGA1 in regulation of stomatal movement and drought resistance of plants

A technology of pyruvate and mitochondria, applied in the fields of genetic engineering and molecular biology, to achieve the effect of improving drought stress resistance and great application value

Active Publication Date: 2016-02-03
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, after searching, there is no report on the application of the corn mitochondrial pyruvate transporter gene ZmNRGA1 in regulating stomatal movement and plant drought resistance.

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  • Application of maize mitochondrion pyruvic acid transporter gene ZmNRGA1 in regulation of stomatal movement and drought resistance of plants
  • Application of maize mitochondrion pyruvic acid transporter gene ZmNRGA1 in regulation of stomatal movement and drought resistance of plants
  • Application of maize mitochondrion pyruvic acid transporter gene ZmNRGA1 in regulation of stomatal movement and drought resistance of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Obtaining of Maize Mitochondrial Pyruvate Transporter Gene ZmNRGA1 Transgenic Plants

[0018] 1. Cloning the ZmNRGA1_T02 gene fragment by PCR method

[0019] Using the cDNA of maize inbred line B73 as a template, PCR amplification was performed with the following primer combinations:

[0020] ZmNRGA1-BamH1-F: 5'-CGGGATCCCGATGGCTGCTACAAAGCTTCA-3'

[0021] ZmNRGA1-Kpn1-R: 5'-GGGTACCCTTACAACTTAAACTGATTGAGC-3'

[0022] After electrophoresis of the PCR product, the gel was cut and recovered, and the ZmNRGA1_T02 gene fragment was purified.

[0023] 2. Vector Ligation and Transformation

[0024] First, connect the purified ZmNRGA1_T02 gene fragment to the intermediate cloning vector Blunt (product of Beijing Quanshijin Company), transform Escherichia coli DH5α, and obtain positive clones through antibiotic screening and colony PCR; The nucleotide sequence is completely correct.

[0025] The ZmNRGA1_T02 nucleotide sequence was excised from the Blunt vector by com...

Embodiment 2

[0036] Example 2 Transgenic application of corn mitochondrial pyruvate transporter gene ZmNRGA1_T02

[0037] Using ZmNRGA1_T02 overexpression plants (OE1, OE5), the following physiological trait experiments were carried out to analyze the application of ZmNRGA1_T02 in regulating stomatal movement and plant drought resistance in Arabidopsis or other economic crops.

[0038] 1. Stomatal Movement Experiment in Arabidopsis

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Abstract

The invention discloses application of maize mitochondrion pyruvic acid transporter gene ZmNRGA1 in regulation of stomatal movement and drought resistance of plants, wherein the maize mitochondrion pyruvic acid transporter gene ZmNRGA1 is a maize pyruvic acid mitochondrion transporter gene ZmNRGA1_T02. Proved by comprehensive experiments, the ZmNRGA1_T02 has an effect of a negative regulatory factor of regulating stomatal movement of plant hormone abscisic acid (ABA), and meanwhile has a regulating effect on plants against drought stress. The application disclosed by the invention is predicted to favorably improve the drought stress capability of plants, has an important theoretical guidance meaning of cultivating drought-resistant high-yield new species of crops and has a huge application value in the agricultural production in China.

Description

technical field [0001] The present invention relates to the application of a corn mitochondrial pyruvate transporter gene, in particular to the application of a corn mitochondrial pyruvate transporter gene ZmNRGA1 (GeneID: 100273562) in regulating stomatal movement and plant drought resistance, belonging to molecular biology, gene field of engineering technology. Background technique [0002] The published maize mitochondrial pyruvate transporter gene ZmNRGA1 gene sequence (GeneID: 100273562) was analyzed by bioinformatics, indicating that it encodes a mitochondrial pyruvate transporter (Mitochondrial Pyruvate Carrier, MPC). It transports pyruvate, the end product of cytosolic glycolysis, into the mitochondria for the tricarboxylic acid cycle. [0003] According to the Corn Research website ( http: / / www.maizegdb.org ) provided eFPBlowser results showed that the ZmNRGA1 gene was expressed in large quantities in plant leaves and other parts. Because the stomata of plant epi...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/00
Inventor 张伟吴琪琪李春龙王美吴晓萌乔柱张尚立
Owner SHANDONG UNIV
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