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High-performance liquid chromatography of phosphatidylserine

A technology of high performance liquid chromatography and phosphatidylserine, which is applied in the field of high performance liquid chromatography analysis of phosphatidylserine, and can solve problems such as monitoring methods and lack of quality of phosphatidylserine

Inactive Publication Date: 2016-02-03
烟台燕园科玛健康产业有限公司
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AI Technical Summary

Problems solved by technology

At present, the content detection method of phosphatidylserine has not been reported, so there is a lack of quality monitoring methods for phosphatidylserine

Method used

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Embodiment Construction

[0018] In a preferred embodiment of the present invention, a high-performance liquid chromatography analysis method for phosphatidylserine is provided, using high-performance liquid chromatography with an evaporative light scattering detector, and using an external standard peak area method to determine the content of phosphatidylserine; specifically including Follow the steps below:

[0019] 1) Prepare phosphatidylserine standard solution, dissolve it with chloroform-methanol solution and set the volume to 80-400ug / ml;

[0020] 2) Prepare a phosphatidylserine sample solution, dissolve and constant volume with chloroform-methanol solution, dilute and prepare a sample solution whose phosphatidylserine content is within the concentration range of the standard series solution;

[0021] 3) Standard solution and sample solution are measured under the conditions of high performance liquid chromatography and evaporative light scattering detector. After the logarithm of the peak area ...

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Abstract

The invention relates to a high-performance liquid chromatography of phosphatidylserine. The High-performance liquid chromatography comprises the steps of (1) preparing a phosphatidylserine standard solution, and using a chloroform-methanol solution to dissolve and fix the constant volume to be 80 to 400ug / ml; (2) preparing a phosphatidylserine sample solution, using the chloroform-methanol solution to dissolve and fix the volume, and diluting and preparing the sample solution of which the phosphatidylserine content is within the concentration range of standard serial solutions; (3) measuring the standard solution and the sample solution under the condition that the high-performance liquid chromatography cooperates with an evaporative light-scattering detector, performing logarithmetics on the peak area of the standard solution as a y-coordinate, performing logarithmetics on the concentration of the standard solution as an x-coordinate, drawing a double logarithmic standard curve, and finding the concentration of the sample solution from the double logarithmic standard curve according to the peak area. The high-performance liquid chromatography has the following beneficial effects: due to the fact that the high-performance liquid chromatography cooperates with the evaporative light-scattering detector, a simple and effective method is provided for the quality control during a phosphatidylserine production process through the entering of different mobile phase volume ratios.

Description

technical field [0001] The invention relates to a high performance liquid chromatography analysis method for phosphatidylserine. Background technique [0002] Phosphatidylserine has a significant effect on improving memory and cognitive ability, preventing and treating Alzheimer's disease, depression and relieving mental stress. It is a functional health care raw material with great development potential, especially in terms of improving brain function. It has the function of protecting nerve cells and improving the function of brain signal transduction. At present, there is no report on the detection method of phosphatidylserine content, so there is a lack of quality monitoring method for phosphatidylserine. [0003] Therefore, it is necessary to propose an effective technical solution to solve the above problems. Contents of the invention [0004] The present invention aims at the deficiencies of the above-mentioned prior art, and provides a high-performance liquid chr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 陈学沛荣艳红修旺珊
Owner 烟台燕园科玛健康产业有限公司
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