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Collagen/calcium alginate/chitosan composite hemostatic sponge and making method

A calcium alginate and collagen technology, which is applied in medical science, absorbent pads, bandages, etc., can solve the problems of irritation, poor biocompatibility, poor biocompatibility, etc., to promote the production of thrombin, and the preparation method is simple. , The effect of high liquid absorption

Inactive Publication Date: 2016-02-17
NAVY MEDICINE RES INST OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen sponge uses a large area of ​​structure to destroy platelets to promote coagulation. The dissolution and degradation of collagen sponge on the wound surface changes the local viscosity of the wound surface, thereby promoting the coagulation process. However, the biocompatibility of animal-derived collagen is generally poor.
Calcium alginate sponge is a functional wound dressing with high hygroscopicity. After contacting with wound exudate, calcium alginate is converted into sodium alginate through ion exchange. The hemostatic effect of alginate dressing is not only due to its coagulation effect, but also because it has a significant stimulating effect on platelet activity, but its biocompatibility is not as good as chitosan sponge

Method used

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  • Collagen/calcium alginate/chitosan composite hemostatic sponge and making method
  • Collagen/calcium alginate/chitosan composite hemostatic sponge and making method
  • Collagen/calcium alginate/chitosan composite hemostatic sponge and making method

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Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: In vitro single factor coagulation experiment of collagen, calcium alginate and chitosan solution

[0032] Prepare 5mg / ml, 10mg / ml, 15mg / ml, 20mg / ml, 25mg / ml collagen solutions (0.1mol / L acetic acid physiological saline solution is the solvent). Add 0.5ml of each to a test tube, bathe in water at 37°C for 1h, measure the coagulation time by the Lee-White whole blood coagulation method, select the one with the shortest coagulation time as the optimal collagen hemostatic concentration, and parallelize three times for each sample. The optimal coagulation concentration of collagen is 15mg / ml.

[0033] Prepare 0.0mg / ml, 0.4mg / ml, 2.0mg / ml, 10mg / ml calcium alginate solution (0.1mol / L acetic acid saline solution as solvent). Add 0.5ml of each to a test tube, bathe in water at 37°C for 1h, measure the coagulation time by the Lee-White whole blood coagulation method, choose the one with the shortest coagulation time as the optimal hemostatic concentration of calci...

Embodiment 2L9

[0035] Embodiment 2L9 (3 3 ) Orthogonal experiment to screen the best formula

[0036] According to the optimum concentration of the collagen protein that embodiment 1 obtains, calcium alginate and chitosan, design L9 (3 3 ) Orthogonal experiment (Table 1, Table 2), prepare three compound solutions with different concentrations, prepare sponges (thickness is 4mm) by freeze-drying, cut 1cm×1cm size respectively, put them in a beaker for 5 minutes at 37°C, Immediately add 0.05ml of fresh sodium citrate anticoagulated rabbit blood to the sample, and immediately add 0.02ml of 0.2mol / L CaCl 2 Solution, incubate at 37°C for 5 minutes, add 25ml of deionized water, shake at 100rpm for 5 minutes, take the supernatant to measure the absorbance, according to the formula BCI=(A 样品 / A 参考 )×100 to calculate the coagulation index, assuming that the absorbance value of the negative control group is 100, each sample was parallelized three times. Through visual analysis (Table 3) and varian...

Embodiment 3

[0044] The preparation of embodiment 3 composite hemostatic sponge

[0045] According to the best recipe obtained in Example 2, a certain amount of collagen was added into the acetic acid saline solution and stirred to prepare a solution with a final concentration of 20 mg / ml; then a certain amount of calcium alginate was added to make the concentration 0.4 mg / ml; finally add a certain amount of chitosan to make the concentration 0.1mg / ml; vortex oscillation for 15min to mix evenly, let stand for 30min to balance, pour into a plastic plate, freeze-dry, take out to obtain a composite hemostatic sponge ( Such as figure 1 ). Sealed and stored to get the product.

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Abstract

The invention relates to the technical field of local trauma hemostatic dressing, in particular to a collagen / calcium alginate / chitosan composite hemostatic sponge and a making method. The composite hemostatic sponge is characterized by being prepared from dissolving collagen, calcium alginate and chitosan according to a certain proportion and adopting a freeze-drying method. After the composite hemostatic sponge contacts with the surface of a wound, blood platelets can be quickly gathered to form blood scab; the composite hemostatic sponge absorbs a lot of exudate due to good liquid absorbing performance to apply certain pressure on the wound so as to play a certain role in pressing hemostasis; a lot of Ca+ in the composite hemostatic sponge can quickly exchange with Na+ in blood after the composite hemostatic sponge contacts with the surface of the wound, so that thrombin generation is promoted, and blood coagulation of the surface of the wound is accelerated. The composite hemostatic sponge can be used for body wound hemostasis.

Description

1 technical field [0001] The invention relates to the technical field of hemostatic dressing for local trauma, in particular to a hemostatic biomaterial-collagen / calcium alginate / chitosan composite hemostatic sponge and a preparation method thereof. 2 background technology [0002] With the development of society, clinical and war wound treatment have higher and higher requirements for local trauma hemostasis. Biomedical materials are considered to be one of the most effective materials for local trauma hemostasis. They have the advantages of absorbability, high efficiency, economy and non-toxicity. Biomedical materials are a new type of hemostatic materials. Blood, condenses platelets, blocks blood vessels, promotes thrombin production, and thus achieves hemostasis. [0003] At present, the advantages of using biomedical materials as local trauma hemostasis have been widely recognized, and its application prospects are promising, and it is receiving widespread attention fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/32A61L15/42
Inventor 沈先荣宗杰刘琼陈伟何颖李佳媚李娜张俊玲王庆蓉蒋定文
Owner NAVY MEDICINE RES INST OF PLA
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