A method for tissue culture and rapid propagation of Yunnan cherry adult fine single plant "Guangzhou" cherry
An excellent technology for rapid propagation of tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of tissue culture buds yellowing and falling leaves, poor quality of seedlings, and few effective buds, etc. The effect of strong root seedlings, short cultivation period and fast induction speed
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Embodiment 1
[0028] A method for tissue culture and rapid propagation of an excellent adult single plant 'Guangzhou' cherry of Yunnan cherry, comprising the following steps:
[0029] (1) Explant collection: From March to May when the weather is clear at around 10:00 in the morning, the semi-lignified shoots with robust growth and no pests and diseases were cut from the 'Guangzhou' cherry tree, and brought back to the laboratory after moisturizing treatment. The collected semi-lignified shoots were defoliated, washed with purified water, and stored as explants in a 4°C refrigerator for later use. Before collecting explants, sprout shoots were sprayed alternately with carbendazim and chlorothalonil 4 to 5 times every 7 days.
[0030] (2) Surface disinfection of explants: Cut the branches (explants) into stems with leaf axils about 4cm long, wash them with sterile water on the ultra-clean workbench, and then use a mass fraction of 0.5% Xinjier The sterilization solution, the soaking time is ...
Embodiment 2
[0040] A method for tissue culture and rapid propagation of an excellent adult single plant 'Guangzhou' cherry of Yunnan cherry, comprising the following steps:
[0041] (1) Explant collection: From March to May, when the weather is clear at about 10 am, the semi-lignified shoots with robust growth and no pests and diseases were cut from the mother plant of 'Guangzhou', and brought back to the laboratory after moisturizing treatment. The collected semi-lignified shoots were defoliated, washed with purified water, and stored as explants in a 4°C refrigerator for later use. Before collecting explants, sprout shoots were sprayed alternately with carbendazim and chlorothalonil 4 to 5 times every 7 days.
[0042] (2) Disinfection of the explant surface: Cut the branches (explants) into stems with leaf axils about 4cm long, wash them with sterile water on the ultra-clean workbench, and then use 1% Xinjier by mass fraction. The sterilizing solution, the soaking time is 5min, after p...
Embodiment 3
[0052] A method for tissue culture and rapid propagation of an excellent adult single plant 'Guangzhou' cherry of Yunnan cherry, comprising the following steps:
[0053] (1) Explant collection: From March to May, when the weather is clear at about 10 am, the semi-lignified shoots with robust growth and no pests and diseases were cut from the mother plant of 'Guangzhou', and brought back to the laboratory after moisturizing treatment. The collected semi-lignified shoots were defoliated, washed with purified water, and stored as explants in a 4°C refrigerator for later use. Before collecting explants, sprout shoots were sprayed alternately with carbendazim and chlorothalonil 4 to 5 times every 7 days.
[0054] (2) Surface disinfection of explants: Cut the branches (explants) into stems with leaf axils about 4cm long, wash them with sterile water on the ultra-clean workbench, and then use a mass fraction of 0.5% Xinjier The sterilization solution, the soaking time is 6min, after...
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