New type nucleic acid detection method and detector tube

Abstract

The invention discloses a new type nucleic acid detection method. Lysate, cleanout fluid and reaction liquid in a detector tube are isolated by arranging a plurality of separating layers in the detector tube, meanwhile, through the control on an external heat source, the separating layers are respectively melted in different detection stages so as to control time sequence in all detection stages, a mixing device which is controlled by an external magnetic body to move and can be magnetized is used in the lysate, mixes samples added into the detector tube, and nucleic acid extraction and purification of the samples are realized, then nano magnetic beads in the detector tube are driven by the external magnetic body to pass the cleanout fluid and then enter into the reaction liquid, nucleic acid carried by the nano magnetic beads is purified in the cleanout fluid and then is eluted in the reaction liquid, an amplified reaction of the nucleic acid in the reaction liquid occurs, finally, an external device realizes the detection on the sample nucleic acid by the optical detection, so that a plurality of steps of performing extraction, purification, cleaning, elution and amplified reaction on the nucleic acid in one detector tube are realized.

Description

technical field [0001] The invention relates to a novel nucleic acid detection method and a detection tube, in particular to a nucleic acid detection method and a detection tube mainly used in nucleic acid extraction and amplification technologies. Background technique [0002] Laboratory testing is an important means of screening and diagnosing bacterial or viral infections, mainly including microscopy, culture, immunological testing and nucleic acid testing. Microscopic examination is low in cost, simple, and convenient, but the positive rate is low and it is easy to miss; the results of culture method are highly reliable, but the cycle is long and the cost is high; immunological detection is easy to operate, but there is a window period, so it is easy to miss The nucleic acid detection cycle is short, and the sensitivity and specificity are comparable to the culture method, but there are the following problems: ①The operation process of sample preparation (nucleic acid ex...

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Problems solved by technology

Microscopic examination is low in cost, simple, and convenient, but the positive rate is low and it is easy to miss; the results of culture method are highly reliable, but the cycle is long and the cost is high; immunological detection is easy to operate, but there is a window period, so it is easy to miss The nucleic acid detection cycle is short, and the sensitivity and specificity are comparable to the culture method, but there are the following problems: ①The operation process of sample preparation (nucleic acid extraction), nucleic acid amplification and amplified product detection is separated, which requires the operation of professional operators There are many and cumbersome steps, which increases operational errors; ②Excessive manual operations can easily cause cross-contamination of samples and false positive test results; ③Most nucleic acid amplification reagents need cold chain transportation and cryopreservation, and the cost is relatively high. high

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