Armeniaca sibirica tissue culture medium, preparation method and application thereof

A tissue culture and culture medium technology, applied in the field of plant tissue culture, can solve the problems of not conforming to the factory seedling raising system, genetic variation of isolated seedlings, and unsuitability of apricots, etc., so as to improve the efficiency of rooting induction and the breeding process. , high rooting rate, shorten the effect of cultivation time

Inactive Publication Date: 2016-03-02
HEBEI NORMAL UNIV FOR NATTIES
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Problems solved by technology

The method of described tissue culture has more successful experience, but apricot is difficult to take root, and this method is not quite applicable to apricot
[0007] Liu Xiaolei and others published in "Tissue Culture of Siberian Apricot" in the third issue of Volume 44 of Plant Physiology Communications, which introduced the tissue regeneration culture of apricot, but what they used

Method used

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Comparison scheme
Effect test

Embodiment

[0038] (1) first prepare the rooting basic medium of apricot, use 1 / 2MS medium, add 5.0g / L agar, 30g / L sucrose, plant growth regulator IBA2.5-3.0mg / L, pH value is adjusted to 5.8, Sterilize at 121°C for 17 minutes for later use;

[0039] (2) Dilute the wood vinegar made from apricot husks by 1500-2500 times, add it into the basic medium for rooting after sterilization, and wait for it to solidify for later use, so as to obtain the rooting medium for tissue cultured seedlings of apricot apricot;

[0040] (3) Inoculate the rooting medium prepared by (2) on the ultra-clean workbench through pretreatment, first-generation culture, and subculture 2-3 generations of apricot tissue culture seedlings, and observe the rooting of apricot tissue culture seedlings Condition.

[0041]Generally, roots can be observed after 7 days, and the rooting situation of the apricot tissue culture seedlings can be recorded at any time. After 15 days of rooting, the rooting rate of the apricot tissue c...

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Abstract

The invention relates to the field of plant tissue culture, in particular to an armeniaca sibirica tissue culture medium and application thereof. The rotting culture medium comprises a 1/2MS culture medium, agar with the concentration being 4-8 g/L, cane sugar with the concentration being 25-50 g/L, a plant growth regulating agent IBA with the concentration being 2-4 mg/L and 1500-3000-fold almond shell wood vinegar. A novel, simple and efficient armeniaca sibirica tissue culture seedling rooting method is built, armeniaca sibirica tissue culture seedlings good in rooting performance and high in rooting yield are obtained through quick and efficient induction, large-scale rooting quick induction of the armeniaca sibirica tissue culture seedlings is achieved under the condition of manual control, armeniaca sibirica tissue culture seedlings rooting induction efficiency is greatly improved, and the seedling culture process is greatly promoted.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to an apricot tissue culture medium, its preparation method and application. Background technique [0002] Armenia casibirica (L.) Lam is a plant of the genus Apricot in the family Rosaceae. It is a woody grain and oil economic tree species integrating ecological, social and economic benefits. The mountain apricot production area lists it as a key economic forest tree species for economic development, which is an important guarantee for economic development and farmers' income increase. However, the development of the mountain apricot industry is hindered by the lack of varieties and cultivation management. [0003] Pingquan County, Hebei Province is planning the reform of the apricot planting system. The introduction of good varieties is a necessary condition, but the cost of purchasing new varieties of seedlings is high, and there is no introduction test in Chengde, so there a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘晓光
Owner HEBEI NORMAL UNIV FOR NATTIES
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