Armeniaca sibirica tissue culture medium, preparation method and application thereof

Abstract

The invention relates to the field of plant tissue culture, in particular to an armeniaca sibirica tissue culture medium and application thereof. The rotting culture medium comprises a 1 / 2MS culture medium, agar with the concentration being 4-8 g / L, cane sugar with the concentration being 25-50 g / L, a plant growth regulating agent IBA with the concentration being 2-4 mg / L and 1500-3000-fold almond shell wood vinegar. A novel, simple and efficient armeniaca sibirica tissue culture seedling rooting method is built, armeniaca sibirica tissue culture seedlings good in rooting performance and high in rooting yield are obtained through quick and efficient induction, large-scale rooting quick induction of the armeniaca sibirica tissue culture seedlings is achieved under the condition of manual control, armeniaca sibirica tissue culture seedlings rooting induction efficiency is greatly improved, and the seedling culture process is greatly promoted.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to an apricot tissue culture medium, its preparation method and application. Background technique [0002] Armenia casibirica (L.) Lam is a plant of the genus Apricot in the family Rosaceae. It is a woody grain and oil economic tree species integrating ecological, social and economic benefits. The mountain apricot production area lists it as a key economic forest tree species for economic development, which is an important guarantee for economic development and farmers' income increase. However, the development of the mountain apricot industry is hindered by the lack of varieties and cultivation management. [0003] Pingquan County, Hebei Province is planning the reform of the apricot planting system. The introduction of good varieties is a necessary condition, but the cost of purchasing new varieties of seedlings is high, and there is no introduction test in Chengde, so there a...

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Problems solved by technology

The method of described tissue culture has more successful experience, but apricot is difficult to take root, and this method is not quite applicable to apricot

[0007] Liu Xiaolei and others published in "Tissue Culture of Siberian Apricot" in the third issue of Volume 44 of Plant Physiology Communications, which introduced the tissue regeneration culture of apricot, but what they used was callus regeneration seedlings. "Regenerated plants" use protoplast regenerated seedlings, which are not collected from the mother body, and are obtained through primary culture and subculture. Whether these isolated seedlings have undergone genetic variation is not conclusive, and it is not in line with the establishment of an industrial seedling cultivation system. the goal of