Unlock instant, AI-driven research and patent intelligence for your innovation.

Extraction method of infectious bronchitis egg yolk antibodies

A technology for bronchitis and chicken infectiousness, which is applied in the field of extraction of egg yolk antibody for chicken infectious bronchitis, can solve the problems of low yield and purity, and achieve the effects of strong biological activity, high purity and yield, and easy popularization and application

Inactive Publication Date: 2016-03-02
TIANJIN RINGPU BIO TECH
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a method for extracting chicken infectious bronchitis egg yolk antibody, so as to solve the technical problem of low yield and purity of related methods in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take chicken eggs from hens immunized against infectious bronchitis, immerse them in 0.1% bromogeramine aqueous solution at 42°C for disinfection for 15 minutes, then immerse the eggs in water with a temperature above 95°C for 5 seconds, take them out and dry them in the air or blow them under aseptic conditions. Dry, beat the eggs to separate the egg white and yolk, fully remove the egg white and yolk mesangium, collect the yolk in a container, stir evenly, and obtain the egg yolk.

[0030] 1) Pretreatment of raw materials: Stir chicken yolk and purified water in a certain proportion evenly;

[0031] 2) Preliminary extraction of two-phase aqueous phase: slowly add 15% volume of saturated ammonium sulfate solution, add PEG to make the mass fraction reach 12%, stir for 20 minutes, let stand for 15 minutes, and collect the upper phase;

[0032] 3) Two-phase extraction and purification: slowly add 15% volume of saturated ammonium sulfate solution to the above solution, sti...

Embodiment 2

[0036] Take chicken eggs from hens immunized against infectious bronchitis, immerse them in 0.1% bromogeramine aqueous solution at 42°C for disinfection for 15 minutes, then immerse the eggs in water with a temperature above 95°C for 5 seconds, take them out and dry them in the air or blow them under aseptic conditions. Dry, beat the eggs to separate the egg white and yolk, fully remove the egg white and yolk mesangium, collect the yolk in a container, stir evenly, and obtain the egg yolk.

[0037] 1) Pretreatment of raw materials: Stir chicken yolk and purified water in a certain proportion evenly;

[0038] 2) Preliminary extraction of two-phase aqueous phase: slowly add 30% volume of saturated ammonium sulfate solution, add PEG to make the mass fraction reach 20%, stir for 30 minutes, let stand for 20 minutes, and collect the upper phase;

[0039] 3) Two-phase extraction and purification: slowly add 30% volume of saturated ammonium sulfate solution to the above solution, sti...

Embodiment 3

[0043] A method for extracting chicken infectious bronchitis egg yolk antibody, comprising the following steps:

[0044] 1) Take the egg yolk of chicken IBH-free, and mix it with water evenly in a ratio of 1:5 (v / v) to obtain the egg yolk dilution;

[0045] 2) Add 10% (v / v) saturated ammonium sulfate solution and PEG to the egg yolk dilution described in step 1) to obtain the first extraction system, then stir for 10 minutes, then let stand for 10 minutes, and collect the upper phase, which is the first upper extraction system Phase, the PEG content in the first extraction system is 8% (w / w);

[0046] 3) Add 10% (v / v) saturated ammonium sulfate solution to the first upper phase described in step 2) to obtain a second extraction system, then stir for 10 minutes, then let stand for 10 minutes, and collect the upper phase to be the second upper phase ; Add 10% (v / v) saturated ammonium sulfate solution to the second upper phase to obtain a third extraction system, adjust its pH t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an extraction method of infectious bronchitis egg yolk antibodies. According to the extraction method, hyper-immune egg yolk is diluted with an appropriate amount of purified water, two aqueous phase extraction is adopted for primary extraction, and two times of extraction purifying are carried out, and then the egg yolk antibodies are purified as far as possible via combination of double-effect ultrafiltration desalting and concentration. The egg yolk antibodies are not destroyed in the extraction process, and biological activity is not influenced. Purity and yield of products obtained via the extraction method are relatively high, biological activity is relatively high, the infectious bronchitis egg yolk antibodies can be made into powder, solution, spray, injection, and injection free dried powder which are suitable for clinical application, and popularization and application are convenient.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a method for extracting chicken infectious bronchitis yolk antibody. Background technique [0002] Avian infectious bronchitis is an acute and highly contagious respiratory infectious disease of chickens caused by infectious bronchitis virus. IBV is a member of the order Nidoviridae, the family Coronaviridae, the genus Coronaviridae, and group III of Coronaviridae. The disease is mainly transmitted through the air, and can also be transmitted through feed, drinking water, litter, etc. The infection of laying hens usually results in a decrease in egg production and a decrease in egg quality. The disease is widely prevalent all over the world and is an important disease in the poultry industry. [0003] Chicken yolk antibody (Immunoglobulinofeggyolk, IgY) is an immunoglobulin that is transferred from the IgG in the blood of the mother poultry to the yolk an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/02C07K1/34C07K1/30
Inventor 高华义李亚杰杨保收付旭彬梁武
Owner TIANJIN RINGPU BIO TECH