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Bristle array biofilm monitoring system and method

A technology of array biology and monitoring system, applied in the fields of environment, materials and public health, can solve the problems of not reflecting the mechanical properties of biofilms, complex formation mechanism of biofilms, and affecting the degree of resistance to physical and chemical methods of biofilm processing.

Active Publication Date: 2018-06-26
杨亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these devices help to control the formation of biofilm to a certain extent, these devices do not have the ability to remove biofilm by themselves. When the biofilm accumulated on the surface exceeds a certain level, it may affect its real-time detection of biofilm and need to be replaced.
[0005] The formation mechanism of biofilm is extremely complex, and its mechanical characteristics directly affect its resistance to various physical and chemical methods of biofilm treatment. Existing biofilm detection devices cannot reflect the mechanical characteristics of biofilm

Method used

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  • Bristle array biofilm monitoring system and method
  • Bristle array biofilm monitoring system and method
  • Bristle array biofilm monitoring system and method

Examples

Experimental program
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Embodiment 1

[0050] Such as figure 1Shown, the bristle array biofilm monitoring system of the present embodiment comprises bristle array sensor 1 and intelligent terminal 2, and described bristle array sensor 1 passes line (as USB, serial port UART, bus SPI, I 2 C, etc.) or wireless signals (such as WIFI, Bluetooth, etc.) are connected with the smart terminal 2;

[0051] The bristle array sensor 1 is installed in different pipeline equipment for sensing the growth of biofilm, and sends the information of the biofilm to the intelligent terminal 2; the bristle array sensor 1 includes a fluorescent magnetic polymer bristle array 3, a CCD (Charge-coupled Device) lens 4 and magnetic field generating device 5;

[0052] The fluorescent magnetic polymer setae array 3 is in contact with the liquid in the environment in the pipeline equipment, so that the setae are in contact with the biofilm; the fluorescent magnetic polymer setae array 3 is distributed with setae with a diameter of 0.5 μm-10 μm a...

Embodiment 2

[0076] This embodiment is the experiment of the setae array biofilm monitoring system of the above-mentioned embodiment 1, and the specific process is as follows:

[0077] S1. Resuscitate bacteria Shewanella oneidensis MR-1, take the strain MR-1 frozen at -20°C, inoculate it on LB agar medium by streaking, and incubate in an incubator (30°C) for 20~ 24h, the next day, pick a monoclonal colony, inoculate in 2mL nutrient broth, shake the bacteria at 180rpm, 30°C overnight;

[0078] S2. After diluting the bacterial solution after overnight culture at 1:100 in fresh broth medium, inoculate it in a multi-well plate with a fluorescent magnetic polymer bristle array embedded at the bottom, and culture it statically in an incubator (30°C) for 24 hours To the formation of biofilm, the fluorescent magnetic polymer setae array that forms the biofilm is used as the fluorescent magnetic polymer setae array of the experimental group, such as Figure 5 Shown; while the fluorescent magnetic ...

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Abstract

The invention discloses a bristle array biofilm monitoring system and method. The system includes a bristle array sensor and an intelligent terminal. The bristle array sensor is connected to the intelligent terminal through lines or wireless signals; the bristle array sensor is installed on different pipeline equipment. , used to sense the growth of the biofilm and send the biofilm information to the intelligent terminal; the intelligent terminal is used to receive the information of the biofilm, analyze the information of the biofilm, and decide whether to Controlling bristle array sensors for biofilm clearance. The system of the present invention installs bristle array sensors in different pipeline equipment, senses the growth of biofilm, and sends biofilm information to an intelligent terminal. After the intelligent terminal analyzes the biofilm information, it obtains the analysis results and decides Whether the magnetic field generating device is controlled to remove the biofilm formed on the surface of the bristle array sensor can be used permanently.

Description

technical field [0001] The invention relates to a biofilm monitoring system and method, in particular to a setae array biofilm monitoring system and method, belonging to the fields of materials, environment and public health. Background technique [0002] At present, biofilm (biofilm) is a highly organized structure formed by microorganisms in nature, some industrial production environments, and the surface of human body, animals and plants through adhesion growth and self-secretion of extracellular matrix (Extracellular polymeric substances, EPS). Multicellular community; biofilm can be formed by pure species or a mixture of microorganisms of various genera, which contain a large amount of water and extracellular matrix secreted by different microorganisms, such as proteins, polysaccharides, DNA, etc.; formed in industry, living pipelines and medical treatment Biofilms in instrument pipelines can cause corrosion, block pipelines, reduce heat transfer, contaminate food, caus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H04N7/18G01N21/84
CPCG01N21/8422H04N7/18
Inventor 周素娟杨亮陈军
Owner 杨亮