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A method for transplanting grape tissue culture seedlings suitable for dry areas

A technology for tissue culture seedlings and grapes, which is applied to botany equipment and methods, cultivation, plant protection covers, etc., can solve the problems of reducing irrigation, excessive spraying, and high humidity of the substrate, and achieves low requirements for facilities and equipment, and transplanting High survival rate and good consistency

Active Publication Date: 2018-05-25
宁夏林业研究院股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are two commonly used methods to solve this problem: ①The temperature and humidity control system is installed in the greenhouse for transplanting grape tissue culture seedlings, and the air temperature and humidity in the greenhouse are adjusted by spraying at regular intervals. If the air humidity is the standard, it is easy to cause too much spray volume, which will make the humidity of the substrate too high, which will cause the transplanted grape tissue culture seedlings to rot easily and reduce the survival rate of transplanting. At the same time, this measure has relatively high requirements for supporting facilities and equipment. High; ② After the grape tissue culture seedlings are transplanted, a small shed is set up to keep moisture. This method can not only ensure the air humidity required for transplanting the grape tissue culture seedlings in the arid area of ​​Northwest China, but also reduce irrigation to ensure that the humidity of the transplanting substrate is moderate, improving The survival rate of transplanted grape tissue-cultured seedlings, but this method can only be applied in spring in the arid area of ​​Northwest China. For high-temperature summer and autumn, due to the high temperature in the small shed, it is impossible to transplant grape tissue-cultured seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Spring transplanting of grape virus-free test-tube seedlings (early April-end of May).

[0030] (1) Transplanted varieties: Cabernet Sauvignon, Vidal, Red Globe;

[0031] (2) Transplanting steps:

[0032] ① Seedling hardening:

[0033] Grape tissue culture seedlings with a plant height of 5-8 cm and 2-3 roots are moved from the cultivation room to the transplanting greenhouse for seedling hardening. During the seedling hardening period, the temperature in the greenhouse is controlled at 15-32°C, and the relative humidity is controlled at 60%-70%. , the two can be separated by lifting the bottle cap) to harden the seedlings for 1d, then open the bottle cap, and cover the bottle mouth with double-layer pure cotton gauze with a humidity of 30-70%, and the density of the gauze is (23-28) × (18 ~24) branches, harden the seedlings for 3~4 days until the color of the leaves of the grape tissue cultured seedlings changes from light green to green or dark green.

[0034] ② S...

Embodiment 2

[0041] Summer and autumn transplanting of grape virus-free test-tube seedlings (early June-early October).

[0042] (1) Transplanted varieties: Cabernet Sauvignon, Vidal, Red Globe;

[0043] (2) Transplanting steps:

[0044] ① Seedling hardening:

[0045] Same as step 1 of embodiment 1;

[0046] ② Seedling washing and transplanting:

[0047] The same as step ② of Example 1, but the formula of the mixed liquid used for quick dipping of roots: 0.2 mg / L IBA, 50 μL / L hymexazol, and 0.6 mg / L carbendazim for medium speed dipping.

[0048] ③Covering and moisturizing:

[0049] It is the same as step ③ of Example 1, but the number of gauze replacements is 6 times each time, and the covering time is 10-12 days;

[0050] ④ Late management and protection:

[0051] Same as step ④ of embodiment 1.

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PUM

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Abstract

The invention relates to a grape tissue culture seedling transplanting method for drought regions; the method comprises the following steps: 1, hardening seedling; 2, washing seedling and transplanting; 3, covering and moisture retention; 4, anaphase management. The grape virus free tissue culture seedling transplanting method for northwest drought regions has the following advantages: 1, using compound rooting agent and bactericide to fast dip the roots of to be transplanted grape virus free tissue culture seedling, thus promoting the rooting of the grape virus free tissue culture seedling, and effectively preventing roots or root collars of transplanted grape virus free tissue culture seedling from rotting; 2, using a dual-layer wet pour cotton cloth to cover the grape virus free tissue culture seedling so as to satisfy the air humidity demands in the transplanting process, thus solving the matrix humidity increasing problems caused by spray adjustment of air humidity. In addition, the method has low requirements for facility equipment, and is simple in operation.

Description

technical field [0001] The invention relates to a method for transplanting grape tissue cultured seedlings suitable for arid regions. Background technique [0002] Grape tissue culture seedling transplanting technology plays a vital role in the industrial production of grape tissue culture seedlings, and is one of the key technologies that determine the success or failure of grape tissue culture seedling industrial production. There have been a lot of reports on the transplanting of grape tissue cultured seedlings, and the transplanting survival rate of grape tissue cultured seedlings can reach more than 90%, but it is limited to small-scale test results. For a single batch of more than 10,000 grape tissue cultured There is no report on the survival of seedlings transplanted. In the process of transplanting grape tissue culture seedlings, the transplanted tissue culture seedlings have higher requirements on the temperature and humidity of the air and the substrate, especial...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G17/02A01G13/02
CPCA01G9/0299A01G13/0243A01G17/02
Inventor 徐美隆刘玉娟谢军陈春伶王巧珍乔改霞
Owner 宁夏林业研究院股份有限公司
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