A kind of corn mushroom production method and product
A corn mushroom and production method technology, applied in mushroom cultivation, botanical equipment and methods, horticulture, etc., can solve the impact of corn mushroom harvest quality on sales, processing, and consumption, affect the market acceptance of corn mushrooms, and have minimal utilization value and other problems, to achieve the effect of simple operation, meeting the needs of life, and high yield of mushrooms
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Embodiment 1
[0028] In this experiment, the isolation and cultivation of smut fungus was carried out in the headquarters of Jiangsu Academy of Agricultural Sciences, and the field inoculation was carried out in Liuhe Animal Science Base, including the following steps:
[0029] (1) Collection and cultivation of corn mushroom strains: Corn smut fungus was collected from Nanjing, Jiangsu, and a small amount of juice from the heart of the mycelium was picked up with a sterile needle under aseptic conditions and spread on PDA medium for 28 Cultivate for 5 days at ℃, transfer all the grown colonies to a 2 ml sterile centrifuge tube, add appropriate amount of sterile water to fully shake the suspended cells, and finally add glycerol to the suspension to make the concentration of glycerol 15%. Store in -80°C refrigerator for long-term storage.
[0030] PDA medium preparation method: 200 no potatoes, peeled and cut into small pieces, boiled in sterile water for 30 minutes, added 20 grams of sucrose...
Embodiment 2
[0036] In this experiment, the isolation and cultivation of smut fungus was carried out in the headquarters of Jiangsu Academy of Agricultural Sciences, and the field inoculation was carried out in Liuhe Animal Science Base, including the following steps:
[0037] (1) Collection and cultivation of corn mushroom strains: Corn smut fungus was collected from Nanjing, Jiangsu, and a small amount of juice from the heart of the mycelium was picked up with a sterile needle under aseptic conditions and spread on PDA medium for 28 Cultivate for 5 days at ℃, transfer all the grown colonies to a 2 ml sterile centrifuge tube, add appropriate amount of sterile water to fully shake the suspended cells, and finally add glycerol to the suspension to make the concentration of glycerol 15%. Store in -80°C refrigerator for long-term storage.
[0038] (2) Preparation of the inoculum: the smut fungus was cultivated before the corn flowering to prepare the inoculum. Take an appropriate amount of c...
Embodiment 3
[0043] In this experiment, the isolation and cultivation of smut fungus was carried out in the headquarters of Jiangsu Academy of Agricultural Sciences, and the field inoculation was carried out in Liuhe Animal Science Base, including the following steps:
[0044](1) Collection and cultivation of corn mushroom strains: Corn smut fungus was collected from Nanjing, Jiangsu, and a small amount of juice from the heart of the mycelium was picked up with a sterile needle under aseptic conditions and spread on PDA medium for 28 Cultivate for 5 days at ℃, transfer all the grown colonies to a 2 ml sterile centrifuge tube, add appropriate amount of sterile water to fully shake the suspended cells, and finally add glycerol to the suspension to make the concentration of glycerol 15%. Store in -80°C refrigerator for long-term storage.
[0045] (2) Preparation of the inoculum: the smut fungus was cultivated before the corn flowering to prepare the inoculum. Take an appropriate amount of co...
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