Application of GSPE (grape seed proanthocyanidin extract) in preparation of targeted drug for treating cell calcium overload injury

A calcium overloading and cellular technology, which is applied in the field of medicine, can solve the problem that the metabolism of calcium ions in cells has not been reported, and achieve the effect of reversing the damage caused by calcium overloading in cells

Inactive Publication Date: 2016-03-23
孙荣距
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  • Description
  • Claims
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Problems solved by technology

Grape seed proanthocyanidin extract GSPE (grapeseed procyanidin extract, GSPE) has anti-oxidation and anti-inflammatory effects, but there is no report on the role of calcium ion metabolism in cells

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  • Application of GSPE (grape seed proanthocyanidin extract) in preparation of targeted drug for treating cell calcium overload injury
  • Application of GSPE (grape seed proanthocyanidin extract) in preparation of targeted drug for treating cell calcium overload injury
  • Application of GSPE (grape seed proanthocyanidin extract) in preparation of targeted drug for treating cell calcium overload injury

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with the embodiments. The following descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention to other forms. Changes to equivalent embodiments with equivalent changes. Any simple modifications or equivalent changes made to the following embodiments according to the technical essence of the present invention without departing from the solution content of the present invention fall within the protection scope of the present invention.

[0020] In this study, a BALB / c mouse model with defective Orai1 gene expression was constructed by gene knockout method, and the sepsis model of intraperitoneal infection of BALB / c mice was replicated by cecal ligation and perforation CLP method. Calcium imaging and molecular biology methods were used to determine that Orai1-mediated SOCE was the main mechanism involved in calcium overload injury in sepsis c...

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Abstract

The invention discloses an application of GSPE (grape seed proanthocyanidin extract) in preparation of a targeted drug for treating cell calcium overload injury. The cell calcium overload injury means vascular endothelial cell calcium overload injury resulting in pyemia, the GSPE does not influence classic signal channels TLR4 and NFkB of LPS (lipopolysaccharide), Orail signals are specifically blocked, and calcium overload signals are blocked. The application indicates that the GSPE can effectively block activation of Orail mediated SOCE (store-operated calcium entry) by LPS, blocks extracellular calcium ion inflow initiated by the Orail mediated SOCE, reverses lung microvascular endothelial cell calcium overload injury of mice with pyemia and reduces the death rate of the mice with pyemia.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to an application of GSPE in targeted medicine for treating cell calcium overload damage. Background technique [0002] Store-operated calcium / calcium release activated calcium (SOC / CRAC) mediated by calcium channel Orai1 is the main mechanism of non-excitatory calcium influx. Bacteremia and endotoxemia caused by Gram-negative bacteria and the endotoxins they release directly act on vascular endothelial cells, resulting in damage and dysfunction of vascular endothelial cells, increased capillary permeability, release of inflammatory mediators, and initiation of pus The occurrence of sepsis, so the damage of vascular endothelial cells is the initiating signal of sepsis, which is the central link. Based on this background, we made an innovative discovery: the store-operated calcium influx mechanism (store-operated calcium entry, SOCE) mediated by Orai1 directly causes cal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/87A61P31/04A61P9/14
CPCA61K36/87
Inventor 孙荣距肖建国陈威黎檀实张文涛
Owner 孙荣距
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