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Composition for degrading acid brilliant green

A technology of acidic bright green and composition, which is applied in the direction of special compound water treatment, water pollutants, biological water/sewage treatment, etc., and can solve the problems of refractory degradation and invisible

Inactive Publication Date: 2016-03-23
林康艺
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Acid Brilliant Green GS is a commonly used dye in the industry. Because it is difficult to degrade, it has become a research problem. There are no research reports on the degradation of Acid Brilliant Green GS by fungal laccase.

Method used

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  • Composition for degrading acid brilliant green
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Examples

Experimental program
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Effect test

Embodiment 1

[0018] Strain: Panus conchatus conchatus, stored in PDA slant medium at 4°C.

[0019] Citric acid-sodium citrate buffer solution: Accurately weigh solid citric acid and sodium citrate, add distilled water to dissolve them, and dilute them to 1L respectively. and mixed to obtain buffers of different pH.

[0020] Acid Brilliant Green GS Mother Liquor: Weigh 0.1g of Acid Brilliant Green GS, dissolve it in distilled water, and finally dilute to 100mL with a volumetric flask. During the experiment, the mother liquor was properly diluted as needed.

[0021] 1-Hydroxybenzotriazole (HOBT) solution: prepare 1% HOBT solution, weigh 1g of HOBT, dissolve in heated distilled water, and dilute to 100mL after cooling. Store at room temperature.

[0022] Preparation of crude enzyme solution: (1) Preparation of seeds: Take out the mature mycelia stored on the PDA slope in the refrigerator, pick the mycelium with an inoculation needle into a masher, add an appropriate amount of distilled wat...

Embodiment 2

[0028] Example 2 Orthogonal Experimental Design of Degradation of Acid Brilliant Green GS by C. conchiformis Laccase

[0029] In this study, 5 factors (pH of the reaction system, reaction temperature, laccase dosage, mediator dosage and dye concentration) were selected, and each factor was divided into 3 levels. According to the selected factors and levels, an orthogonal table is designed using SPSS software.

[0030]

[0031] According to the factor and level requirements, run the IBMSPSSStatistics program, enter the data editing window, click "Data-Orthogonal Design-Generate", open the "Generate Orthogonal Design" dialog box, enter A in the "Factor Name" box, and click Add; Enter B, C, D, E, F, then select variable A, click "Define Value" to enter the "Generate Design: Define Value" dialog box, and enter 1, 2, 3. It means factor A has 3 levels, click "Continue" to return to the main dialog box of "Generate Orthogonal Design" at the previous level, input the three levels ...

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Abstract

The invention belongs to the technical field of dye degradation, and discloses a composition for degrading acid brilliant green. The composition comprises a crude enzyme fluid and 1-hydroxybenzotriazole, wherein the crude enzyme fluid is fermented from Panus conchatus; the crude enzyme fluid fermented from Panus conchatus and 1-hydroxybenzotriazole are utilized and mixed under the optimal reaction condition for 35 min, and the degradation rate to acid brilliant green reaches 98%.

Description

technical field [0001] The invention relates to the technical field of dye degradation, in particular to a composition for degrading acid brilliant green. Background technique [0002] In addition to the white paper used in office and printing in the paper industry, the demand for a large amount of colored paper is also increasing with the improvement of people's living standards and the rapid development of the economy. Dyeing dyes, resulting in a large amount of dye wastewater. [0003] Dye wastewater is colored wastewater containing dyes, which is one of the most difficult industrial wastewater to treat. With the rapid development of the dye industry and printing industry, the types and quantities of dyes are constantly increasing. During the production and use of dyes, about 10-20% of dyes are discharged into the environment with wastewater. The basic raw materials for the production of dyes are benzene series, naphthalene series, anthraquinone, aniline and benzidine ...

Claims

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Application Information

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IPC IPC(8): C02F3/34
CPCC02F3/342C02F2101/308C02F2103/28C02F2305/04
Inventor 林康艺
Owner 林康艺
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