Composition for degrading acid brilliant green
A technology of acidic bright green and composition, which is applied in the direction of special compound water treatment, water pollutants, biological water/sewage treatment, etc., and can solve the problems of refractory degradation and invisible
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Embodiment 1
[0018] Strain: Panus conchatus conchatus, stored in PDA slant medium at 4°C.
[0019] Citric acid-sodium citrate buffer solution: Accurately weigh solid citric acid and sodium citrate, add distilled water to dissolve them, and dilute them to 1L respectively. and mixed to obtain buffers of different pH.
[0020] Acid Brilliant Green GS Mother Liquor: Weigh 0.1g of Acid Brilliant Green GS, dissolve it in distilled water, and finally dilute to 100mL with a volumetric flask. During the experiment, the mother liquor was properly diluted as needed.
[0021] 1-Hydroxybenzotriazole (HOBT) solution: prepare 1% HOBT solution, weigh 1g of HOBT, dissolve in heated distilled water, and dilute to 100mL after cooling. Store at room temperature.
[0022] Preparation of crude enzyme solution: (1) Preparation of seeds: Take out the mature mycelia stored on the PDA slope in the refrigerator, pick the mycelium with an inoculation needle into a masher, add an appropriate amount of distilled wat...
Embodiment 2
[0028] Example 2 Orthogonal Experimental Design of Degradation of Acid Brilliant Green GS by C. conchiformis Laccase
[0029] In this study, 5 factors (pH of the reaction system, reaction temperature, laccase dosage, mediator dosage and dye concentration) were selected, and each factor was divided into 3 levels. According to the selected factors and levels, an orthogonal table is designed using SPSS software.
[0030]
[0031] According to the factor and level requirements, run the IBMSPSSStatistics program, enter the data editing window, click "Data-Orthogonal Design-Generate", open the "Generate Orthogonal Design" dialog box, enter A in the "Factor Name" box, and click Add; Enter B, C, D, E, F, then select variable A, click "Define Value" to enter the "Generate Design: Define Value" dialog box, and enter 1, 2, 3. It means factor A has 3 levels, click "Continue" to return to the main dialog box of "Generate Orthogonal Design" at the previous level, input the three levels ...
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