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Universal SSR marker tightly linked with dominant genic male sterility genes in broccoli

A male sterility gene and cell nucleus technology, applied in the field of molecular biology, can solve the problems of unverified, difficult gene location, poor versatility, etc., and achieve the effect of shortening the breeding cycle, less restrictive factors, and improving efficiency.

Active Publication Date: 2016-03-23
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the above-mentioned markers have poor versatility and are only applicable to individual populations of their respective studies, and these markers are all single-flanked markers with large genetic distances, making it difficult to perform gene mapping
Chinese kale BC obtained by backcrossing with this dominant sterile source by Zhang Xinmei et al. 4 The segregated population was used as material, and 8 SRAP and 1 SSR markers were screened to be closely linked to the dominant sterility gene, and the linkage distances of the nearest double-flanked markers ENA14F-CoEm7R and 8C0909 were 0.53cM and 2.55cM respectively (Zhang Xinmei et al. , "Molecular markers closely linked to dominant male sterility gene CDMs399-3 in cabbage", Chinese Agricultural Sciences, No. 11, 2009), but these markers have not been verified by other groups, and their applicability cannot be judged. Molecular markers closely linked to dominant nuclear male sterility gene in broccoli have not been reported

Method used

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  • Universal SSR marker tightly linked with dominant genic male sterility genes in broccoli
  • Universal SSR marker tightly linked with dominant genic male sterility genes in broccoli
  • Universal SSR marker tightly linked with dominant genic male sterility genes in broccoli

Examples

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Effect test

Embodiment 1

[0037] Example 1 Screening of the universal SSR molecular marker SSR10312a closely linked to the dominant nuclear male sterility gene in broccoli and the identification of the broccoli sterility gene using the SSR molecular marker SSR10312a

[0038] The test materials used in this example are broccoli inbred lines 8554, 90196, 93219 and 94174, and their corresponding dominant male sterile lines DGMS8554, DGMS90196, DGMS93219 and DGMS94174. The four test materials of broccoli inbred lines 8554, 90196, 93219 and 94174 are high-generation inbred lines bred by the cabbage and broccoli research group of the Institute of Vegetable and Flower Research, Chinese Academy of Agricultural Sciences through multi-generation self-selection; DGMS8554, DGMS90196, DGMS93219 and DGMS94174 are broccoli dominant male sterile lines bred by crossing the dominant nuclear male sterile material with the corresponding inbred line through high-generation (n≥9 generations) backcross breeding; these germpla...

Embodiment 2

[0055] Example 2. Verification of BDMs gene flanking markers

[0056] The marker SSR10312a linked to the BDMs gene obtained in Example 1 was verified using 10 dominant nuclear male sterility materials obtained from BDMs gene-containing and different broccoli backcross breeding males, so as to determine the markers for molecular-assisted selection accuracy. Compared with the field phenotypes of the selected materials, the phenotype data reflected by the band patterns of the marker in 10 materials were consistent with the field investigation results, and the accuracy rate was 100%.

[0057] In this example, the genetic distance between the universal SSR marker (SSR10312a) closely linked with the dominant nuclear male sterility gene and BDMs in broccoli is BC of DGMS8554 10 (including 747 individual plants) and BC of DGMS90196 11 (Containing 338 individual plants) The genetic distances in the segregated populations are 0.563cM and 0.532cM respectively, this SSR marker closely l...

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Abstract

The invention relates to a universal SSR molecular marker SSR10312a tightly linked with dominant genic male sterility genes in broccoli. A primer pair for amplifying the molecular marker SSR10312a by PCR is as shown in Seq ID No.1-2. The invention further provides application of the SSR molecular marker in a broccoli germplasm resource having dominant genic male sterility genes. The SSR molecular marker can be used for performing assistant selection on a broccoli candidate material in the process of transferring the dominant genic male sterility genes at any period of the broccoli candidate material, has the advantages of high efficiency, few limiting factors and the like, and can be used for improving the efficiency of selecting dominant genic male sterility genes of broccoli and shortening the breeding cycle.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a general SSR marker closely linked with a dominant nuclear male sterility gene in broccoli and its application in identifying the dominant nuclear male sterility gene in broccoli. Background technique [0002] Broccoli (Brassica oleracea var. italica), also known as broccoli and Italian kale, is an important characteristic vegetable sold in the international market with green curd as the product of Brassica genus Brassica. It has rich nutrition and unique anti-inflammatory properties. Cancer efficacy, favored by the majority of consumers. In recent years, the cultivation area and area of ​​broccoli in my country have been increasing continuously, and it has become the main vegetable and an important export vegetable in Zhejiang, Gansu, Yunnan, Hebei and other provinces. [0003] While the demand for broccoli products is increasing, the domestic broccoli seed market is facing se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘玉梅舒金帅李占省方智远杨丽梅庄木张扬勇吕红豪孙培田
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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