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Cottonseed gossypol content non-destructive measurement method based on near-infrared spectrum technology

A technology of near-infrared spectroscopy and near-infrared spectrometer, which is applied in the field of determination of agricultural and sideline products, can solve the problems of time-consuming and laborious, polluting the environment, and increasing the nutritional evaluation of cottonseed, and achieves the reduction of spectral signal-to-noise ratio, high measurement accuracy, and reliable Effect of Recovery Analysis

Inactive Publication Date: 2016-04-13
ZHEJIANG UNIV
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Problems solved by technology

Determination of gossypol content according to routine is time-consuming and laborious, and requires a large amount of toxic chemical reagents, which is expensive and pollutes the environment, which adds difficulty to the nutritional evaluation of cottonseed
In addition, the traditional chemical method of shelling and grinding to analyze the gossypol content in cottonseed will inevitably consume test materials, and the test samples cannot be recycled, which limits its application in improving the nutritional quality of cottonseed

Method used

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  • Cottonseed gossypol content non-destructive measurement method based on near-infrared spectrum technology
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  • Cottonseed gossypol content non-destructive measurement method based on near-infrared spectrum technology

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specific Embodiment

[0028] 1) Selection of samples:

[0029] The samples were delint cottonseeds from 11 regions across the country in 2012, 2013 and 2014, including multiple varieties from different ecological planting areas such as Hangzhou in Zhejiang Province, Wuhu in Anhui Province, Yancheng in Jiangsu Province, and Lixian County in Hunan Province. A total of 268 cottonseed samples were collected.

[0030] 2) Sample Spectrum Collection

[0031] The delint cotton seeds were loaded into the sample cell, and each sample was scanned four times to obtain the spectral average value. Sample raw spectrogram, such as figure 1 shown.

[0032] Near-infrared spectrum acquisition method: at room temperature 25±0.5°C, use a near-infrared spectrometer at a wave number of 4000-10000cm -1 In the near-infrared spectrum range, a total of 1501 spectral points, every 4cm -1 Collect reflection intensity (R). Each loaded sample was scanned 64 times in the near-infrared spectrometer, and stored in the computer...

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Abstract

The invention discloses a cottonseed gossypol content non-destructive measurement method based on the near-infrared spectrum technology. Different cottonseed samples are collected, the spectrum of the cottonseed samples is collected through a near-infrared spectrograph, near-infrared spectrum data are preprocessed through various spectrum preprocessing methods, the gossypol content of the samples is precisely measured through an efficient liquid chromatography method, a correction model is constructed through the partial least square method, an optimal near-infrared spectrum correction model is determined through predictive related coefficients, residual predictive deviations, predictive root mean square errors and cross validation root mean square errors, the cottonseed samples to be measured are detected through the optimal near-infrared spectrum correction model, and the gossypol content of the cottonseed samples to be measured is obtained. By means of the near-infrared spectrograph, a spectrogram of cottonseeds is acquired, measurement speed is high, precision is high, chemical reagents are not needed, the method is non-destructive, green and capable of quickly analyzing the gossypol content in the cottonseeds, and the method plays an important role in developing and utilizing cottonseed by-products and cultivating new cotton varieties.

Description

technical field [0001] The invention discloses a method for measuring the content of agricultural by-products. It specifically relates to a non-destructive determination method of gossypol content in cottonseed based on near-infrared spectroscopy, using near-infrared spectroscopy and chemometric multivariate correction technology to perform non-destructive determination of gossypol content in whole cottonseeds. Background technique [0002] Cottonseed is the main by-product of cotton, which has a wide range of uses. Its output is about 1.5 times that of cotton fiber. The annual output of cottonseed in the world can reach 28-30 million tons. Fat (28.24-44.05%) is a potential source of protein and edible oil. China is the largest cottonseed producer in the world, with an annual production capacity of 8 million tons of cottonseed, 1.48 million tons of extractable cottonseed oil and 1.4 million tons of cottonseed protein. In addition, the composition of each amino acid in cott...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/3563
CPCG01N21/359G01N21/3563
Inventor 祝水金李诚赵茹冰陈进红
Owner ZHEJIANG UNIV
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