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Method for determining toxicity of pesticide suspending agent by adopting daphnia magna

A large Daphnia and suspension agent technology, applied in the field of breeding, can solve the problems of lack of research data to evaluate the practicability and reliability of the model, and less research, and achieve the effect of strong practicability, high accuracy, and easy operation

Inactive Publication Date: 2016-04-13
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, daphnia magna has been widely used in the research of environmental safety assessment of pesticides, but there are few studies using daphnia magna as a toxicity screening model for pesticide suspensions, and there is a lack of relevant research data to evaluate the practicability and reliability of the model

Method used

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  • Method for determining toxicity of pesticide suspending agent by adopting daphnia magna
  • Method for determining toxicity of pesticide suspending agent by adopting daphnia magna
  • Method for determining toxicity of pesticide suspending agent by adopting daphnia magna

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of pesticide stock solution:

[0025] Weigh 1.1905g of 21% thiamethoxam suspension, use ElendtM 4 Dissolve the culture solution and dilute it to a 100mL volumetric flask, and prepare a concentration of 2.50×10 3 mga.i. / L pesticide stock solution.

[0026] (2) Preparation of test solution:

[0027] Elendt M 4 Preparation of culture medium: ElendtM 4 The culture solution was aerated by the air aeration pump for more than 24 hours, and the ElendtM 4 The pH of the culture solution was 7.18, the hardness was 175 mg / L, and the dissolved oxygen was 7.83.

[0028] Experimental group: pipette the stock solution 0.100mL, 0.180mL, 0.324mL, 0.583mL, 1.050mL in turn. 4 Dilute the culture solution to a 250mL volumetric flask to obtain a concentration of 1.00mga.i. / L, 1.80mga.i. / L, 3.24mga.i. / L, 5.83mga.i. / L, 10.5mga.i. / L of the test solution.

[0029] Blank control group (CK 1 ): to ElendtM 4 The culture solution was used as the test solution.

[0030] (3)...

Embodiment 2

[0049] (1) Preparation of pesticide stock solution:

[0050] Weigh 0.0833g of 30% hexaconazole suspension, use ElendtM 4 The culture solution was dissolved and fixed to a 100mL volumetric flask to prepare a pesticide stock solution with a concentration of 250mga.i. / L.

[0051] (2) Preparation of test solution:

[0052] Elendt M 4 Preparation of culture medium: ElendtM 4 The culture solution was aerated by the air aeration pump for more than 24 hours, and the ElendtM4 The pH of the culture solution was 7.23, the hardness was 182 mg / L, and the dissolved oxygen was 6.99 mg / L.

[0053] Experimental group: Pipette 0.100, 0.200, 0.400, 0.800, 1.600, 3.200mL of the pesticide stock solution in step (1) in sequence and use ElendtM 4 Dilute the culture solution to a 250mL volumetric flask to obtain test drug solutions with concentrations of 0.100, 0.200, 0.400, 0.800, 1.60, and 3.20 mga.i. / L. The experimental group set up 4 repetitions.

[0054] Blank control group (CK 1 ): to El...

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Abstract

The invention discloses a method for determining the toxicity of a pesticide suspending agent by adopting daphnia magna. The method comprises the following steps: dissolving the pesticide suspending agent with an Elendt M4 culture solution to prepare a pesticide stock solution; mixing the pesticide stock solution with the Elendt M4 culture solution to prepare a mixed solution with a gradient concentration; taking the mixed solution as domestic water of the daphnia magna and continuously culturing for 48 hours; in a culturing process, recording biological effect parameters of the daphnia magna and physicochemical parameters of the mixed solution every 24 hours; and calculating the median inhibitory concentration EC50 of a pesticide according to the biological effect parameters of the daphnia magna and the physicochemical parameters of the mixed solution, and determining the toxicity of the pesticide suspending agent. The method provided by the invention has the advantages of simplicity and convenience for operation, strong practicability, rapid detection speed, high accuracy and the like.

Description

technical field [0001] The invention relates to the field of breeding technology, in particular to a method for determining the toxicity of a pesticide suspension in Daphnia magna. Background technique [0002] Daphnia magna is a crustacean Cladocera invertebrate widely distributed in the world. It feeds on phytoplankton and is also a bait for fish and large invertebrates. It is an important source of material circulation and energy flow in aquatic ecosystems. link. Due to its wide distribution, fast reproduction, short life cycle, easy cultivation and domestication, sensitivity to poisons in the environment and simple experimental methods, it has been valued by toxicologists in many countries. It has become a standard test organism and is widely used in aquatic toxicology research. [0003] At present, Daphnia magna has been widely used in the research of environmental safety assessment of pesticides, but there are few studies using Daphnia magna as a toxicity screening m...

Claims

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Application Information

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IPC IPC(8): G01N33/00
CPCG01N33/00
Inventor 刘勇陈昂张德咏罗香文严清平刘建宇罗源华
Owner HUNAN PLANT PROTECTION INST
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