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Gerrardanthus macrorhiza aseptic seedling and culture method thereof

A cultivation method and technology of sleeping cloth bag, which is applied in the field of sleeping cloth bag tissue culture, can solve the problems of low reproduction coefficient, limited number of acquisitions, difficulty in obtaining sleeping cloth bag seeds, etc., and achieve high quality, short cultivation period, reproduction coefficient and reproduction efficiency excellent effect

Inactive Publication Date: 2016-04-20
ANHUI NORMAL UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The common propagation methods of sleeping cloth bags include cut propagation and sowing methods. According to the existing reports, it is difficult to obtain seeds for sowing and propagation of sleeping cloth bags. It is greatly affected by environmental factors and other problems, while other breeding methods have problems such as low reproduction coefficient and limited efficiency

Method used

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  • Gerrardanthus macrorhiza aseptic seedling and culture method thereof
  • Gerrardanthus macrorhiza aseptic seedling and culture method thereof
  • Gerrardanthus macrorhiza aseptic seedling and culture method thereof

Examples

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Effect test

Embodiment 1

[0042] 1) In mid-September 2015, select full sleeping bag seeds and soak them in a jar filled with sterile distilled water (use a rubber band to cover the mouth of the jar with a single layer of gauze) at 23°C for 12 hours, and then Rinse the jar directly in running water for 1 h, and finally dry the water on the surface of the seeds with filter paper.

[0043] 2) Carefully peel off the seed coat with tweezers from the winged side of the seeds, soak the seeds in 75% alcohol solution at 23°C for 30 seconds, then soak them in 15% hydrogen peroxide solution at 23°C for 12 minutes, and finally Wash 5 times with sterile water to obtain sterile explants.

[0044] 3) First use the sterilized tweezers to draw a groove about 1.5cm long and 0.3cm deep on the surface of the culture medium, then place the sterile seeds in the groove so that the seeds fully contact with the culture medium; Then the sterile explants were inoculated into the above-mentioned MS medium with a pH of 6.5, where...

Embodiment 2

[0047] Cultivate according to the method of Example 1 to obtain a sterile seedling with a height of 7 cm. The difference is that the soaking time of the seeds in the hydrogen peroxide solution in step 2) is 9 min.

Embodiment 3

[0049] Cultivate according to the method of Example 1 to obtain a sterile seedling with a height of 3 cm. The difference is that in step 2), the seeds are soaked in hydrogen peroxide solution for 15 minutes.

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Abstract

The invention discloses a gerrardanthus macrorhiza aseptic seedling and a culture method thereof, and the culture method comprises the steps of 1) placing seeds of gerrardanthus macrorhiza into sterile distilled water for soaking; 2) peeling seed coats of the soaked seeds of gerrardanthus macrorhiza, then soaking seeds without seed coats into an alcoholic solution and a hydrogen dioxide solution in sequence, and then washing with the sterile distilled water to obtain sterile explants; 3) inoculating the sterile explants with a culture medium; 4) performing dark culture and culture under the condition of alternation of light and darkness on the culture medium in sequence. The culture method has excellent reproduction coefficient and breeding efficiency and short culture cycle, in addition, the gerrardanthus macrorhiza aseptic seedling cultured by the culture method is fast in growth rate.

Description

technical field [0001] The invention relates to tissue culture of a sleeping bag, in particular to a sleeping bag sterile seedling and a cultivation method thereof. Background technique [0002] Sleeping cloth bag (Gerradanthus macrorrhizus) is a famous succulent bulb ornamental plant of Cucurbitaceae (Cucurbitaceae) sleeping cloth bag genus (Gerradanthus), native to East Africa. Entering this century, succulent plants represented by sleeping bags are sought after by succulent lovers. The perennial sleeping bag plant has attracted much attention among succulents for its huge bulbs and graceful posture. The Institute of Botany, Chinese Academy of Sciences, Jiangsu Province began to introduce sleeping bag seedlings from Africa in 2005 for ground planting and domestication, and successfully harvested seeds. . [0003] The common propagation methods of sleeping cloth bags include cut propagation and sowing methods. According to the existing reports, it is difficult to obtain ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 李真俞剑宇陈夫贵郑艳宋正达刘科伟肖家欣李晴晴
Owner ANHUI NORMAL UNIV
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