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Method for detecting genuineness of cordyceps sinensis stroma powder/powdery flakes in nondestructive mode

A Cordyceps sinensis, non-destructive testing technology, applied in the direction of measuring devices, material analysis through optical means, instruments, etc., can solve the problems of cumbersome operation, sample consumption, high testing cost, etc., and achieve accurate results, easy operation and low cost Effect

Inactive Publication Date: 2016-04-20
QINGDAO SPRING MEDICAL RESOURCE TECH UTILIZATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is: to propose a method for non-destructive detection of the authenticity of Cordyceps sinensis powder / powder sheet, and to solve the problems of cumbersome operation, high detection cost and the need to consume samples in the detection method in the traditional technology

Method used

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  • Method for detecting genuineness of cordyceps sinensis stroma powder/powdery flakes in nondestructive mode
  • Method for detecting genuineness of cordyceps sinensis stroma powder/powdery flakes in nondestructive mode
  • Method for detecting genuineness of cordyceps sinensis stroma powder/powdery flakes in nondestructive mode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] 1. Sample preparation:

[0082] Select 8 samples of Qinghai Guoluo Cordyceps sinensis, intercept the sub-seat part, dry brush, wash, and dry at 40°C in sequence, and the ultrafine powder / powder is crushed into particle size D 90 Particles of ≤25μm are prepared into sub-base powder / powder tablet as the sample to be predicted in this example.

[0083] 2. Sample hyperspectral data collection:

[0084] The sample is sent to the hyperspectral camera lens, according to the set parameters (spectral collection range is short-wave infrared band (SWIR, 940–2537nm), pixel size is 320 (space) × 256 (spectrum), pixel size is 30 μm × 30 μm, using field of view It is a 10mm lens. The scanning method is high-speed push-broom hyperspectral imaging, the push-broom speed is 3mm / s, and the acquisition speed is 100fps.) to scan and save the hyperspectral image and spectral information of the sample. Image processing is performed by the Evince software of Umbio, Sweden, using the camera's ...

Embodiment 2

[0094] 1. Sample preparation:

[0095] Select commercially available fermented mycelium powder / powder A, fermented mycelium powder / powder B, fermented mycelium powder / powder C, fermented mycelium powder / powder D, hemp back Six samples, including Zizuo powder / powder and Yaxiang stick powder / powder, were dried at a low temperature of 40°C as samples to be predicted in this example.

[0096] 2. Sample hyperspectral data collection:

[0097]The sample is sent to the lens of the hyperspectral camera, scanned according to the set parameters (same as in Example 1), and the hyperspectral image and spectral information of the sample are saved. Image processing is performed by the Evince software of Umbio, Sweden, using the camera's built-in black and white standard material to automatically correct the image, and then the light intensity value is converted into a spectral absorption curve by A / D.

[0098] 3. Image processing:

[0099] First delete the data in the 940–1000nm and 2469...

Embodiment 3

[0107] In this example, a certain brand of fermented mycelium powder / powder is used as an additive, which is mixed into pure Cordyceps powder / powder in a certain proportion, and the example is predicted after mixing evenly;

[0108] 1. Sample preparation:

[0109] Select 4 samples: the first is to add 1% fermented mycelium powder / powder to pure Cordyceps sinensis powder / powder; the second is to add 5% content to pure Cordyceps sinensis powder / powder fermented mycelium powder / powder; the third part is to add 10% fermented mycelium powder / powder to pure Cordyceps sinensis powder / powder; the fourth part is to add pure Cordyceps sinensis powder / powder Add 15% fermented mycelium powder / powder;

[0110] The above 4 samples were dried at a low temperature of 40°C as samples to be predicted in this example.

[0111] 2. Sample hyperspectral data collection:

[0112] The sample is sent to the lens of the hyperspectral camera, scanned according to the set parameters (same as in Exampl...

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Abstract

The invention belongs to the field of medicinal material identification, discloses a method for detecting genuineness of cordyceps sinensis stroma powder / powdery flakes in a nondestructive mode and solves the problems that in the traditional technology, the detection modes are complex in operation and high in detection cost, and samples need to be consumed. The method comprises the following steps: 1, selecting a positive sample and a negative sample; 2, collecting hyperspectral images of the samples; 3, processing the hyperspectral images, and extracting relevant characteristics through the principal component analysis method; 4, establishing a PLS-DA prediction model; 5, detecting an object to be detected through the PLS-DA prediction model; 6, judging genuineness of the object to be detected according to output of the model. The method is applicable to rapid and accurate identification conducted on the cordyceps sinensis stroma powder samples.

Description

technical field [0001] The invention belongs to the field of identification of medicinal materials, and in particular relates to a method for non-destructive detection of the authenticity of Cordyceps sinensis powder / powder tablets. Background technique [0002] Cordyceps sinensis (Berkeley) Saccardo is an ascus fungus formed by parasitizing on the larvae of the genus Hepialus of the genus Cordyceps sinensis of the family Ergotaceae. The compound of the larvae corpse of the bat moth of the Lepidoptera Batmothidae insect has a sweet and flat nature, nourishes the lungs and kidneys, stops bleeding and resolves phlegm, and is used for chronic cough and dyspnea, hemoptysis due to fatigue, impotence and spermatorrhea, and sore waist and knees. It is a traditional medicine in my country. precious Chinese herbal medicines. [0003] The growth environment of Cordyceps sinensis is special, and it is mainly distributed in alpine meadows and alpine shrubs at an altitude of 3000-5000 met...

Claims

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Application Information

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IPC IPC(8): G01N21/3563G01N21/359
CPCG01N21/3563G01N21/359G01N2021/3572G01N2201/0618
Inventor 张雪峰谭福元
Owner QINGDAO SPRING MEDICAL RESOURCE TECH UTILIZATION CO LTD
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