Method for detecting genuineness of cordyceps sinensis stroma powder/powdery flakes in nondestructive mode

A Cordyceps sinensis, non-destructive testing technology, applied in the direction of measuring devices, material analysis through optical means, instruments, etc., can solve the problems of cumbersome operation, sample consumption, high testing cost, etc., and achieve accurate results, easy operation and low cost Effect

Inactive Publication Date: 2016-04-20
QINGDAO SPRING MEDICAL RESOURCE TECH UTILIZATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is: to propose a method for non-destructive detection of the authenticity of Cordyceps sinensis powder/

Method used

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  • Method for detecting genuineness of cordyceps sinensis stroma powder/powdery flakes in nondestructive mode
  • Method for detecting genuineness of cordyceps sinensis stroma powder/powdery flakes in nondestructive mode
  • Method for detecting genuineness of cordyceps sinensis stroma powder/powdery flakes in nondestructive mode

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0080] Example 1:

[0081] 1. Sample preparation:

[0082] Select 8 samples of Cordyceps sinensis from Qinghai Guoluo, cut the sub-seat part, dry brush, wash, and dry at 40°C in sequence. The ultrafine powder / flake is broken into particle size D 90 Particles ≤25μm are prepared into sub-seed powder / powder tablets as the sample to be predicted in this example.

[0083] 2. Sample hyperspectral data collection:

[0084] Transfer the sample to the lens of the hyperspectral camera, and press the set parameters (the spectrum collection range is shortwave infrared band (SWIR, 940-2537nm), the pixel is 320 (space) × 256 (spectrum), the pixel size is 30 μm × 30 μm, and the field of view is adopted It is a 10mm lens. The scanning method is high-speed push-broom hyperspectral imaging, the push-scan speed is 3mm / s, and the acquisition speed is 100fps.) Scan to save the hyperspectral image and spectral information of the sample. The image processing is performed by the Evince software of Umbio, Sw...

Example Embodiment

[0093] Example 2:

[0094] 1. Sample preparation:

[0095] Select commercially available fermented mycelium powder / powder sheet A, fermented mycelium powder / powder sheet B, fermented mycelium powder / powder sheet C, fermented mycelium powder / powder sheet D, hemp ridge back Six samples of Zizai powder / powder tablets, Yaxiang Bangzizae powder / powder tablets, etc., were dried at a low temperature of 40°C as the samples to be predicted in this example.

[0096] 2. Sample hyperspectral data collection:

[0097] Send the sample to the lens of the hyperspectral camera, scan according to the set parameters (same as the settings in Example 1), and save the hyperspectral image and spectral information of the sample. Image processing is carried out by the Evince software of Umbio, Sweden, using the camera's built-in black and white standard materials to automatically correct the image, and then the light intensity value is converted into a spectral absorption curve by A / D.

[0098] 3. Image proce...

Example Embodiment

[0106] Example 3:

[0107] In this example, a certain brand of fermented mycelium powder / powder flakes is used as an additive, mixed into pure cordyceps powder / powder flakes in a certain proportion, and mixed evenly.

[0108] 1. Sample preparation:

[0109] Select 4 samples: the first one is to add 1% fermented mycelium powder / powder to pure Cordyceps sinensis powder / powder flakes; the second one is to add 5% content to pure Cordyceps flakes powder / powder flakes The third part is to add 10% fermented mycelium powder / powder to the pure Cordyceps sinensis powder / powder sheet; the fourth part is to add 10% fermented mycelium powder / powder sheet to the pure cordyceps sinensis powder / powder sheet; Add 15% fermented mycelium powder / powder flakes;

[0110] The 4 samples mentioned above were dried at a low temperature of 40°C as samples to be predicted in this example.

[0111] 2. Sample hyperspectral data collection:

[0112] Send the sample to the lens of the hyperspectral camera, scan accor...

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Abstract

The invention belongs to the field of medicinal material identification, discloses a method for detecting genuineness of cordyceps sinensis stroma powder/powdery flakes in a nondestructive mode and solves the problems that in the traditional technology, the detection modes are complex in operation and high in detection cost, and samples need to be consumed. The method comprises the following steps: 1, selecting a positive sample and a negative sample; 2, collecting hyperspectral images of the samples; 3, processing the hyperspectral images, and extracting relevant characteristics through the principal component analysis method; 4, establishing a PLS-DA prediction model; 5, detecting an object to be detected through the PLS-DA prediction model; 6, judging genuineness of the object to be detected according to output of the model. The method is applicable to rapid and accurate identification conducted on the cordyceps sinensis stroma powder samples.

Description

technical field [0001] The invention belongs to the field of identification of medicinal materials, and in particular relates to a method for non-destructive detection of the authenticity of Cordyceps sinensis powder / powder tablets. Background technique [0002] Cordyceps sinensis (Berkeley) Saccardo is an ascus fungus formed by parasitizing on the larvae of the genus Hepialus of the genus Cordyceps sinensis of the family Ergotaceae. The compound of the larvae corpse of the bat moth of the Lepidoptera Batmothidae insect has a sweet and flat nature, nourishes the lungs and kidneys, stops bleeding and resolves phlegm, and is used for chronic cough and dyspnea, hemoptysis due to fatigue, impotence and spermatorrhea, and sore waist and knees. It is a traditional medicine in my country. precious Chinese herbal medicines. [0003] The growth environment of Cordyceps sinensis is special, and it is mainly distributed in alpine meadows and alpine shrubs at an altitude of 3000-5000 met...

Claims

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Application Information

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IPC IPC(8): G01N21/3563G01N21/359
CPCG01N21/3563G01N21/359G01N2021/3572G01N2201/0618
Inventor 张雪峰谭福元
Owner QINGDAO SPRING MEDICAL RESOURCE TECH UTILIZATION CO LTD
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