Microfluidic chip, hemocyte separation method and system, and preparation method of system

Abstract

The invention discloses a microfluidic chip, a whole blood hemocyte separation system based on the chip, a preparation method of the system and a method for separating whole blood hemocytes by using the system, is used for separating the hemocytes, can overcome the defects of low separation efficiency and insufficient miniaturization and lightweight in the prior art, and meets on-site real-time blood sample separation requirements. The chip is prepared by successively bonding a substrate layer containing a main flow channel structure with a microporous filter membrane layer and a flexible polymer layer containing an upper-layer flow channel structure, and a micro-channel system is formed in the chip; the micro-channel system includes a micro-pump area, a circulation area, a first channel, a second channel, a third channel, and a filtering area formed by the main flow channel structure, the microporous filter membrane layer and the upper-layer flow channel structure.

Description

technical field [0001] The invention relates to the technical field of blood cell separation, in particular to a microfluidic chip, a whole blood cell separation system based on the chip, a manufacturing method of the system, and a method for using the system to separate whole blood cells. Background technique [0002] White blood cells contain a wealth of immune system information, and many tests for white blood cells require their isolation from whole blood. Some traditional separation methods, such as centrifugation, require large equipment, large sample requirements, and complicated operations, making it difficult to integrate with subsequent detection and analysis instruments. The use of microfluidic chips to separate white blood cells is the basis for the detection and analysis of white blood cells based on microfluidic chips. Efficient isolation of leukocytes from whole blood in microfluidic chips remains challenging due to the high concentration of blood cells and t...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a microfluidic chip, a whole blood cell separation system based on the chip, a manufacturing method of the system and a method for separating blood cells by using the system, which are used to separate blood cells and can overcome The defects of low separation efficiency, miniaturization and light weight of the existing technology meet the needs of on-site instant blood sample separation

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