Microfluidic chip, hemocyte separation method and system, and preparation method of system

A microfluidic chip and chip technology, which is applied in the field of blood cell separation, can solve the problems of low separation efficiency, miniaturization and insufficient lightweight, and achieve the effects of reducing clogging, realizing light weight, and realizing miniaturization.

Active Publication Date: 2016-05-04
TSINGHUA UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a microfluidic chip, a whole blood cell separation system based on the chip, a manufacturing method of the system and a method for separating blood cells by using the

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  • Microfluidic chip, hemocyte separation method and system, and preparation method of system
  • Microfluidic chip, hemocyte separation method and system, and preparation method of system
  • Microfluidic chip, hemocyte separation method and system, and preparation method of system

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[0050] In order to make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the following will clearly describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are the present invention. Invented some embodiments, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

[0051] Such as Figure 1 to Figure 3 As shown, this embodiment discloses a microfluidic chip. The chip is composed of a microporous filter film layer 13, a substrate layer 11 containing a main flow channel structure (the material is a non-magnetic, transparent material plate) and an upper layer flow The flexible polymer layer 15 of the channel ...

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Abstract

The invention discloses a microfluidic chip, a whole blood hemocyte separation system based on the chip, a preparation method of the system and a method for separating whole blood hemocytes by using the system, is used for separating the hemocytes, can overcome the defects of low separation efficiency and insufficient miniaturization and lightweight in the prior art, and meets on-site real-time blood sample separation requirements. The chip is prepared by successively bonding a substrate layer containing a main flow channel structure with a microporous filter membrane layer and a flexible polymer layer containing an upper-layer flow channel structure, and a micro-channel system is formed in the chip; the micro-channel system includes a micro-pump area, a circulation area, a first channel, a second channel, a third channel, and a filtering area formed by the main flow channel structure, the microporous filter membrane layer and the upper-layer flow channel structure.

Description

technical field [0001] The invention relates to the technical field of blood cell separation, in particular to a microfluidic chip, a whole blood cell separation system based on the chip, a manufacturing method of the system, and a method for using the system to separate whole blood cells. Background technique [0002] White blood cells contain a wealth of immune system information, and many tests for white blood cells require their isolation from whole blood. Some traditional separation methods, such as centrifugation, require large equipment, large sample requirements, and complicated operations, making it difficult to integrate with subsequent detection and analysis instruments. The use of microfluidic chips to separate white blood cells is the basis for the detection and analysis of white blood cells based on microfluidic chips. Efficient isolation of leukocytes from whole blood in microfluidic chips remains challenging due to the high concentration of blood cells and t...

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Application Information

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IPC IPC(8): B01L3/00C12M1/36C12M1/12C12N5/078
CPCB01L3/50273B01L3/502753B01L3/502761B01L2300/0681B01L2300/0819B01L2400/0403C12N5/0634
Inventor 叶雄英成一诺马增帅谢帅张帅
Owner TSINGHUA UNIV
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