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Method for efficiently extracting okra polysaccharide

A polysaccharide and high-efficiency technology, applied in the field of extracting okra polysaccharide, can solve problems such as low extraction rate of okra polysaccharide, and achieve the effect of improving extraction efficiency

Inactive Publication Date: 2016-05-04
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention is the low extraction rate problem of extracting okra polysaccharide from okra in the prior art, and then provides a kind of processing method of extracting okra polysaccharide with high efficiency

Method used

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  • Method for efficiently extracting okra polysaccharide
  • Method for efficiently extracting okra polysaccharide
  • Method for efficiently extracting okra polysaccharide

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Experimental program
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Effect test

Embodiment 1-5

[0044] The method for efficiently extracting okra polysaccharide described in embodiment 1-5, comprises the steps:

[0045] (1) Grind red okra to powder, accurately weigh 10 g of red okra powder, add 650 mL of buffer solution of pH 5.0 prepared according to the above method and mix evenly, and perform ultrasonic extraction for 15 minutes under 140W power ultrasonic assisted conditions;

[0046] (2) Add the compound enzyme that comprises cellulase, pectinase and bovine trypsin in the feed liquid after ultrasonic treatment (in embodiment 1-5, the mass ratio of described cellulase, pectinase and bovine trypsin is respectively 2:2:1, 1:1:0.5, 1:3:2, 3:1:0.5, 3:1:2) mixing, the amount of the compound enzyme accounts for 2.25wt% of the feed liquid quality , and placed in a 60°C water bath for extraction for 2 hours;

[0047] (3) adding boiling water for enzyme inactivation treatment for 15 minutes, and then centrifuging to obtain the supernatant to obtain the crude extract containi...

Embodiment 6-10

[0049] The method for efficiently extracting okra polysaccharide described in embodiment 6-10, comprises the steps:

[0050](1) Get the red okra and grind it to powder, and accurately weigh 10g of the red okra powder, add the buffer solution of pH5. 400mL, 500mL, 600mL, 700mL) and mix well, and perform ultrasonic extraction for 15min under the condition of 180W power ultrasonic assist;

[0051] (2) Add a compound enzyme comprising cellulase, pectinase and bovine trypsin (the mass ratio of the cellulase, pectinase and bovine trypsin is 2:2:1) to the feed liquid after ultrasonic treatment Evenly, the consumption of described complex enzyme accounts for 2wt% of described feed liquid quality, and is placed in 60 ℃ of water-baths, water-bath extracts 30min;

[0052] (3) adding boiling water for enzyme inactivation treatment for 15 minutes, and then centrifuging to obtain the supernatant to obtain the crude extract containing the okra polysaccharide.

Embodiment 11-16

[0054] The method for efficiently extracting okra polysaccharide described in embodiment 11-16, comprises the steps:

[0055] (1) Get okra and grind it to powder, and accurately weigh 10g of okra powder, add 600mL of buffer solution 600mL of pH5. Ultrasonic frequency is 80W, 100W, 120W, 140W, 160W, 180W) for ultrasonic extraction for 15min;

[0056] (2) Add a compound enzyme comprising cellulase, pectinase and bovine trypsin (the mass ratio of the cellulase, pectinase and bovine trypsin is 2:2:1) to the feed liquid after ultrasonic treatment Evenly, the consumption of described complex enzyme accounts for 2wt% of described feed liquid quality, and is placed in 60 ℃ of water-baths, water-bath extracts 30min;

[0057] (3) adding boiling water for enzyme inactivation treatment for 15 minutes, and then centrifuging to obtain the supernatant to obtain the crude extract containing the okra polysaccharide.

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Abstract

The invention belongs to the technical field of natural extracts and relates to a method for efficiently extracting okra polysaccharide, in particular to a process for extracting okra polysaccharide from okra. According to the method, hibiscus coccineus, a kind of okra, is adopted as a raw material, and complex enzymes which comprise cellulose, pectinase and bovine trypsin are adopted for enzymolysis extraction. Compared with such a manner that a single enzyme is adopted for extraction in the prior art, the method has the advantage that the okra polysaccharide extraction efficiency is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of natural extracts, in particular to a process for extracting okra polysaccharides from okra. Background technique [0002] Okra (AbelmoschussculentusL.Moench) has another name called okra, okra, coffee ambrette, hair eggplant, hollyhock, and is also called "pepper pepper" among the people. Native to Africa, it was introduced into China from India in the early 20th century, and is more common in southern China. The edible part is the fruit pod, which is divided into green and red. It tastes crisp, tender and juicy, smooth and not greasy, and has a unique fragrance. The seeds can be squeezed for oil. [0003] Okra is light in taste and cold in nature, and has the effects of relieving sore throat, dredging stranguria, breast milk, and regulating menstruation. Okra is rich in vitamins and minerals. Every 100 grams of okra tender fruit contains about 4 mg of vitamin C, 1.03 mg of vitamin E and 310 micrograms ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 何磊张力金汤卫东王蓉
Owner UNIV OF JINAN
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