The invention discloses a
hibiscus coccineus
tissue culture method and belongs to the technical field of
plant biology. The
hibiscus coccineus
tissue culture method comprises explants preparation and
light quality treatment and concretely comprises selecting mellow and plump
hibiscus coccineus seeds, washing the seeds through flowing water for 1.5-2h, carrying out disinfection through
alcohol with a concentration of 70% for 30s, carrying out disinfection through mercuric
chloride with a concentration of 0.15% for 6-8min, carrying out washing through
sterile water 5-6 times, immersing the seeds in
sterile water for 12-24h, inoculating a 1 / 2 MS with the seeds, carrying out culture under
white light for 5-7 days,
cutting off cotyledons with petiole as explants, inoculating a MS with 0.05mg / L of NAA with the explants, carrying out culture for 20 days,
cutting off a terminal
bud with two true leave as explant, inoculating a MS with 0.10mg / L of NAA and 0.05mg / L of 6-BA with the terminal
bud, and culturing the MS under
light emitting diode (LED) light with blue, red and green combined light according to 3: 1: 1 and carrying out light culture for 35 days. The LED light with blue, red and green combined light according to 3: 1: 1 is suitable for hibiscus coccineus
tissue culture seedling growth. The method has the advantages of simple processes, fast and good
plant growth, energy saving and environmental friendliness, has an important effect in hibiscus coccineus rapid propagation
in vitro or
molecular breeding research and has high actual application value.