Butterfly orchid florescence related GIGANTEA gene (DhGI1) coding sequence and application
A technology for coding sequences and phalaenopsis, which is applied in the application, genetic engineering, plant genetic improvement and other directions to achieve the effects of improving product quality and economic benefits, reducing production costs, and enriching theories and methods of flowering
Inactive Publication Date: 2016-05-04
ZHEJIANG FORESTRY UNIVERSITY
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However, the nucleotide sequence of the Phalaenopsis flower-related GIGANTEA gene (DhGI1) and its application in transgenic plants have not been seen publicly before the publication of the present invention.
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[0030] The test situation relevant to the present application will be introduced below. For the experimental methods that do not indicate specific conditions in the following examples, generally follow conventional conditions, such as the conditions described in "Molecular Cloning Experiment Guide" edited by Sambrook.J. et al., or according to the conditions suggested by the manufacturer.
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The invention belongs to the technical field of molecular biology and gene engineering, particularly provides a coding sequence of floral development related gene DhGI1 expressed in a butterfly orchid and application thereof in plant flowering phase regulation and control and relates to a recombinant plant expression vector including the gene and a transgenic plant obtained through transformation by applying the vector. By applying the gene to perform transformation and expression in the plant, a certain regulation and control effect can be played on the flowering time of the transgenic plant.
Description
technical field [0001] The invention belongs to the field of plant genetic engineering, in particular to the cloning of a flower-related GIGANTEA gene (DhGI1) expressed in Phalaenopsis, the analysis of the gene sequence, the analysis of the gene expression pattern, the construction of the target gene expression vector, and the plant Transformation, obtaining of transgenic plants and identification of corresponding physiological characteristics. Background technique [0002] Phalaenopsis is deeply loved by consumers at home and abroad as a flower with good economic value and ornamental value, but its flower formation needs electricity cooling or high mountain flower forcing, which greatly increases its production cost. Therefore, many scholars try to regulate the flowering period of Phalaenopsis orchids through genetic engineering to reduce production costs. Han Yingying et al. (2004, 2005) obtained the cDNA of chalcone synthase (pchs) in the petals of Phalaenopsis, and succ...
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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
CPCC07K14/415C12N15/827
Inventor 崔永一罗小燕周明兵张迟秦巧平
Owner ZHEJIANG FORESTRY UNIVERSITY