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Method for preparing positive serum of antibody against porcine Japanese encephalitis virus

A technology for swine Japanese encephalitis and positive serum, which is applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as lack of methods, and achieve the effects of high safety, relatively low cost and good immunogenicity

Active Publication Date: 2016-05-04
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a method for preparing porcine Japanese encephalitis virus antibody-positive serum, so as to solve the technical problem of lack of related methods in the prior art

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  • Method for preparing positive serum of antibody against porcine Japanese encephalitis virus
  • Method for preparing positive serum of antibody against porcine Japanese encephalitis virus
  • Method for preparing positive serum of antibody against porcine Japanese encephalitis virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 (preparation of porcine Japanese encephalitis virus antibody positive serum)

[0034] 1 Preparation of the vaccine

[0035] 1.1 Preparation and inspection of porcine Japanese encephalitis live vaccine (SA14-14-2 strain)

[0036] 1.1.1 Preparation of Live Vaccine The JEV venom that has passed the sterility test is added with a suitable freeze-dried protective agent and a suitable antibiotic at the same time. Japanese encephalitis live vaccine (SA14-14-2 strain).

[0037] 1.1.2 Live vaccine test The live vaccine for porcine Japanese encephalitis produced in 1.1.1 is subjected to physical properties, vacuum degree, residual moisture, sterility test, mycoplasma test, exogenous virus test, identification test, safety test and efficacy Inspection (determination of virus content), the results are all qualified. Wherein the virus content of the JEV venom used for the seedling was determined to be 10 8.0 TCID 50 / ml, the virus content after making live porcine ...

Embodiment 2

[0067] Embodiment 2 (preparation of porcine Japanese encephalitis virus antibody positive serum)

[0068] 1 Preparation of the vaccine

[0069] 1.1 Preparation and inspection of porcine Japanese encephalitis live vaccine (SA14-14-2 strain)

[0070] 1.1.1 Preparation of Live Vaccine The JEV venom that has passed the sterility test is added with a suitable freeze-dried protective agent and a suitable antibiotic at the same time. Japanese encephalitis live vaccine (SA14-14-2 strain).

[0071] 1.1.2 Live vaccine test The live vaccine for porcine Japanese encephalitis produced in 1.1.1 is subjected to physical properties, vacuum degree, residual moisture, sterility test, mycoplasma test, exogenous virus test, identification test, safety test and efficacy Inspection (determination of virus content), the results are all qualified. Wherein the virus content of the JEV venom used for the seedling was determined to be 10 8.6 TCID 50 / ml, the virus content after making live porcine ...

Embodiment 3

[0099] Embodiment 3 (preparation of porcine Japanese encephalitis virus antibody positive serum)

[0100] 1 Preparation of the vaccine

[0101] 1.1 Preparation and inspection of porcine Japanese encephalitis live vaccine (SA14-14-2 strain)

[0102] 1.1.1 Preparation of Live Vaccine The JEV venom that has passed the sterility test is added with a suitable freeze-dried protective agent and a suitable antibiotic at the same time. Japanese encephalitis live vaccine (SA14-14-2 strain).

[0103] 1.1.2 Live vaccine test The live vaccine for porcine Japanese encephalitis produced in 1.1.1 is subjected to physical properties, vacuum degree, residual moisture, sterility test, mycoplasma test, exogenous virus test, identification test, safety test and efficacy Inspection (determination of virus content), the results are all qualified. Wherein the virus content of the JEV venom used for the seedling was determined to be 10 8.4 TCID 50 / ml, the virus content after making live porcine ...

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Abstract

The invention provides a method for preparing the positive serum of an antibody against the porcine Japanese encephalitis virus. The method comprises the following steps: vaccine preparation; negative pig screening; immunization; and harvesting and identification of the positive serum. According to the method, a negative pig is basically immunized with a live porcine Japanese encephalitis vaccine (strain SA14-14-2); and then a high-titer inactivated porcine Japanese encephalitis vaccine (strain SA14-14-2) prepared by using concentration and purification technology is used for supplementary immunization so as to prepare the positive serum of the antibody against the porcine Japanese encephalitis virus. The positive serum of the antibody against the porcine Japanese encephalitis virus prepared in the invention avoids the risk of usage of virulent vaccines and has the advantages of high safety compared with preparation of positive serum from virulent Japanese encephalitis vaccines; and compared with serum prepared from mice, the positive serum prepared from the pig has the advantages of a larger harvesting amount of serum, lower cost and prominent large-scale application prospects.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a method for preparing porcine Japanese encephalitis virus antibody-positive serum. Background technique [0002] Japanese encephalitis is a zoonotic arbovirus-borne infectious disease caused by Japanese Encephalitis Virus (JEV) belonging to the genus Flavivirus of the family Flaviviridae. Pigs infected with Japanese encephalitis form viremia, which is considered to be the main source of infection of human Japanese encephalitis. Therefore, preventing and controlling pig Japanese encephalitis can not only reduce the loss of the pig industry, but also prevent people from suffering from Japanese encephalitis. Important measure for encephalitis. It mainly causes reproductive disorders in sows and boars, mainly manifested as abortion in pregnant sows, stillbirth, weak piglets, orchitis in boars, etc., often causing serious economic losses to the pig industry. [0003] Va...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/531G01N21/76
CPCG01N21/76G01N33/531G01N33/56983
Inventor 丁旭娜吕茂杰杨保收付旭彬梁武
Owner TIANJIN RINGPU BIO TECH
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