Method for preparing positive serum of antibody against porcine Japanese encephalitis virus
A technology for swine Japanese encephalitis and positive serum, which is applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as lack of methods, and achieve the effects of high safety, relatively low cost and good immunogenicity
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Embodiment 1
[0033] Embodiment 1 (preparation of porcine Japanese encephalitis virus antibody positive serum)
[0034] 1 Preparation of the vaccine
[0035] 1.1 Preparation and inspection of porcine Japanese encephalitis live vaccine (SA14-14-2 strain)
[0036] 1.1.1 Preparation of Live Vaccine The JEV venom that has passed the sterility test is added with a suitable freeze-dried protective agent and a suitable antibiotic at the same time. Japanese encephalitis live vaccine (SA14-14-2 strain).
[0037] 1.1.2 Live vaccine test The live vaccine for porcine Japanese encephalitis produced in 1.1.1 is subjected to physical properties, vacuum degree, residual moisture, sterility test, mycoplasma test, exogenous virus test, identification test, safety test and efficacy Inspection (determination of virus content), the results are all qualified. Wherein the virus content of the JEV venom used for the seedling was determined to be 10 8.0 TCID 50 / ml, the virus content after making live porcine ...
Embodiment 2
[0067] Embodiment 2 (preparation of porcine Japanese encephalitis virus antibody positive serum)
[0068] 1 Preparation of the vaccine
[0069] 1.1 Preparation and inspection of porcine Japanese encephalitis live vaccine (SA14-14-2 strain)
[0070] 1.1.1 Preparation of Live Vaccine The JEV venom that has passed the sterility test is added with a suitable freeze-dried protective agent and a suitable antibiotic at the same time. Japanese encephalitis live vaccine (SA14-14-2 strain).
[0071] 1.1.2 Live vaccine test The live vaccine for porcine Japanese encephalitis produced in 1.1.1 is subjected to physical properties, vacuum degree, residual moisture, sterility test, mycoplasma test, exogenous virus test, identification test, safety test and efficacy Inspection (determination of virus content), the results are all qualified. Wherein the virus content of the JEV venom used for the seedling was determined to be 10 8.6 TCID 50 / ml, the virus content after making live porcine ...
Embodiment 3
[0099] Embodiment 3 (preparation of porcine Japanese encephalitis virus antibody positive serum)
[0100] 1 Preparation of the vaccine
[0101] 1.1 Preparation and inspection of porcine Japanese encephalitis live vaccine (SA14-14-2 strain)
[0102] 1.1.1 Preparation of Live Vaccine The JEV venom that has passed the sterility test is added with a suitable freeze-dried protective agent and a suitable antibiotic at the same time. Japanese encephalitis live vaccine (SA14-14-2 strain).
[0103] 1.1.2 Live vaccine test The live vaccine for porcine Japanese encephalitis produced in 1.1.1 is subjected to physical properties, vacuum degree, residual moisture, sterility test, mycoplasma test, exogenous virus test, identification test, safety test and efficacy Inspection (determination of virus content), the results are all qualified. Wherein the virus content of the JEV venom used for the seedling was determined to be 10 8.4 TCID 50 / ml, the virus content after making live porcine ...
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