Bacterial strain of Paecilomyces lilacinus and application thereof to production of Pu'er tea

A technology of Paecilomyces lilacinus and its strains, applied in the field of Paecilomyces lilacinus and its application in the production of Pu-erh tea, can solve the problems of lack of in-depth tracking, and achieve the effect of improving the index of pesticide residues

Active Publication Date: 2016-05-25
MENGHAI TEA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the field of Pu-erh tea fermentation research, the early stage focused on basic research, but lacked in-depth tracking of specific applications

Method used

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  • Bacterial strain of Paecilomyces lilacinus and application thereof to production of Pu'er tea
  • Bacterial strain of Paecilomyces lilacinus and application thereof to production of Pu'er tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening of Paecilomyceslilacinus (Paecilomyceslilacinus) TMCC70004 strain

[0024] Fresh fermented tea samples were collected from Pu-erh tea heaps, and Paecilomyceslilacinus TMCC70004 strain was isolated by conventional medium.

[0025] The purified medium is: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1000mL of distilled water, and sterilized at 121°C for 30min.

[0026] The separation and purification method is as follows:

[0027] 1. Culture medium preparation: sterilize the prepared culture at 121°C for 30 minutes, cool to about 45°C, pour into sterile culture dishes, about 20ml per dish, cool and solidify for later use;

[0028] 2. Sample treatment: take the puer tea sample to be separated, grind and pulverize it, take 10g, put it into a conical flask filled with 90ml sterile water in the ultra-clean workbench, shake it to mak...

Embodiment 2

[0033] Embodiment 2 Morphological identification of Paecilomyceslilacinus (Paecilomyceslilacinus) TMCC70004 strain

[0034] Paecilomyceslilacinus (Paecilomyceslilacinus) TMCC70004 bacterial strain of the present invention is streaked and inoculated on Cha's culture medium, begins to grow after cultivating 24 hours, after 4 days, bacterium colony diameter is about 50mm, and bacterium colony shape is regular circle, and the color of bacterium colony outer ring It is white, with lavender in the middle, and the colony turns dark red after 7 days. The growth of mycelium in Bengal, PDA and Chapei medium was the most significant, while that in PDB and PCA medium increased slowly. A large number of spores were produced on the developed hyphae, and the spore production was the largest on the Chashi and oat medium.

Embodiment 3

[0035] The production application of embodiment 3 Paecilomyceslilacinus (Paecilomyceslilacinus) TMCC70004 strain

[0036] Add water to the large-leaf sun-dried green hair tea until the water content reaches about 20%-45%, sterilize at 121°C for 30 minutes, then inoculate the TMCC70004 seed liquid in the sterilized hair tea according to the inoculation amount of 1:20, and place it at 30°C— Incubate at 45°C for several weeks.

[0037] Replenish water and turn the heap on a weekly basis. In the first week, TMCC70004 began to multiply, the number of strains increased geometrically, and the secretion of biologically active substances and enzymes increased. It can promote the formation of Puer tea soup with strong red tea and warm taste, and can degrade the residual pesticides in the raw materials of Puer tea, so as to improve the residual index of pesticides in Puer tea.

[0038] See the data in Table 1 for specific comparison results.

[0039] Table 1 Comparison table of the con...

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Abstract

The invention belongs to the field of production of Pu'er tea, and concretely discloses a bacterial strain of Paecilomyces lilacinus and an application thereof to production of the Pu'er tea. The bacterial strain of Paecilomyces lilacinus is Paecilomyces lilacinus TMCC 70004, and the booked number of the bacterial strain in the China general microbiological culture collection center is CGMCC No.8680. The bacterial strain of Paecilomyces lilacinus is applied to pile fermentation process of the Pu'er tea, and the Pu'er tea soup with red, thick, warm and smooth mouthfeel is obtained; at the same time, the bacterial strain can be used for degrading residual pesticides in the Pu'er tea raw material, in order to improve the index of pesticide residue of the Pu'er tea.

Description

technical field [0001] The invention relates to the field of Pu'er tea production, in particular to a strain of Paecilomyces lilacinus and its application in the production of Pu'er tea. Background technique [0002] With the advancement of food science and technology, as well as the mining and utilization of microbial resources, the selection and application of excellent microbial strains are becoming more and more extensive. [0003] Paecilomyces lilacinus fungus can produce abundant functional enzymes such as chitinase, glucanase and protease, which can promote material changes during the fermentation process of Pu-erh tea, and have a good role in promoting the formation of Pu-erh tea quality. At the same time, the fungus has a certain degree of pesticide degradation. Somaseknar found that Paecilomyces lilacinus fungus can promote the dissolution of insoluble phosphate. Laboratory research results confirmed that the solubilization effect of Paecilomyces lilacinus fungi r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23F3/10C12R1/79
Inventor 唐蜀昆高林瑞田飞卢开阳任万增童一峰黄华伟刘韬邹小林卢晓慧
Owner MENGHAI TEA IND
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